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Citations for GE Life Sciences :
2701 - 2750 of 4416 citations for Dengue Virus Serotype 3 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... cell culture media were clarified by centrifugation and the IgG captured using Protein A resin (GE Healthcare). Proteins were eluted from the resin using citrate buffer pH 3 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... The pull-down of biotinylated proteins was performed using 100 µl StrAv Mag Sepharose™ (GE Healthcare) for each condition and incubated in a rotation wheel for 3h at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... and the eluted proteins were further purified via gel filtration chromatography with a superdex 200 (GE Healthcare) column ...
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bioRxiv - Cell Biology 2023Quote: ... wet electroblotting (100V for 1h) was used to transfer proteins onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were immobilized by blotting them onto an Amersham Protran Premium nitrocellulose membrane (GE Healthcare Life Sciences) in cold 1 x Tris-Glycine ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were pooled and concentrated before separation on a Superdex 200 16/60 column (GE Healthcare) equilibrated in GF2 Buffer (50 mM Tris-HCl pH 7.5 ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then purified using a ResourceTM Q 6ml column (17-1179-01, GE Healthcare, Uppsala, Sweden) and a Superdex 200 Increase 10/300 column (28-9909-44 ...
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bioRxiv - Genetics 2023Quote: ... Protein containing fractions were further purified by size exclusion chromatography using Superdex 200 10/300 (GE Healthcare) gel-filtration column in SEC buffer (20 mM HEPEs pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were concentrated and gel-filtrated on a SuperdexTM 75 size exclusion chromatography column (GE Healthcare) equilibrated with stock buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples with indicated concentrations were loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare, USA) pre-equilibrated with gel filtration buffer (150 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...
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bioRxiv - Immunology 2023Quote: ... The secreted antibodies in the supernatant were purified by protein G Sepharose 4 Fast Flow (GE Healthcare). Purified antibodies were dialyzed in PBS and sterile filtered (0.22 μm) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS/PAGE gel and transferred to Protran nitrocellulose membrane (0.2 μm, GE Healthcare). Immunoblotting was performed by incubating with primary antibodies diluted in Tris-buffered saline (20mM Tris pH 7.6 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare) in NETN buffer (20mM Tris PH8 ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing soluble recombinant protein were pulled-down with Glutathione (GSH) Sepharose 4FastFlow (GE Healthcare, 17-5132) for 3h at 4°C with gently rocking ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified using an anion exchange chromatography column (HiTrap Q HP, 5 mL; GE Life Sciences) followed by ammonium sulfate precipitation (20 to 30% ammonium sulfate saturation ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed proteins were concentrated and purified by SEC (HiLoad Superdex-200 16/600 column, GE Healthcare) into tau buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was transferred to an Amersham™ Hybond™ P0.45 polyvinylidene difluoride (PVDF) membrane (GE Healthcare) in 1x Tris/Glycine transfer buffer (National diagnostics ...
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bioRxiv - Molecular Biology 2023Quote: ... 170 µl of antiserum was coupled to 40 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed extensively in CPB and supplemented with 4 µl fresh rPAS ...
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bioRxiv - Neuroscience 2023Quote: ... the protein was cleaved with Tev protease and purified on a HiTrap Q HP column (GE Healthcare) using a salt gradient ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Acetylated LCas12a proteins were purified following the steps of Ni-NTA column (HisTap™ HP, GE Healthcare), ion exchange chromatography (HiTrap™ SP HP ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed using the described antibodies and protein G- or A- coupled Sepharose beads (GE Healthcare). Beads were washed with high salt buffer (50 mM Tris/Cl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified using nickel affinity chromatography with a 5 ml HisTrap HP column (GE Healthcare) followed by size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was passed through a HiLoad® 26/600 Superdex® 75 pg (GE Healthcare) size exclusion chromatography column and the resulting pure protein was stored in gel filtration buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted proteins were concentrated and loaded onto a Superdex 200 column (10/300 GL; GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The immune-complexes were collected with 50 ul of protein A sepharose (17-0780-01; GE Healthcare) at 4°C for two hours with rotation ...
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bioRxiv - Biophysics 2024Quote: ... Dialysed protein was further purified by ion-exchange chromatography using a HiTrap-FF Q column (GE Healthcare), resulting in the elution of N-myc proteins in the flow-through and at low salt concentrations ...
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bioRxiv - Microbiology 2024Quote: ... Collected supernatant containing the Fc fused nanobody was incubated overnight with protein-A Sepharose beads (GE Healthcare), after which proteins were purified using poly-prep chromatography columns (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was additionally purified by size-exclusion chromatography (Superdex 200 16/60 column; GE Healthcare™).
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bioRxiv - Neuroscience 2024Quote: ... All proteins were further purified by SEC using a Superdex 200 10/300 GL column (GE Healthcare) with 20 mM Tis-HCl at pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... The cleaved proteins were further purified by cation exchange chromatography using HiTrap SP HP column (GE Healthcare) equilibrated in buffer C ...
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bioRxiv - Microbiology 2024Quote: ... The mAbs were isolated from the culture supernatants by affinity chromatography using Protein G beads (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... 25 mg of protein was incubated with 25 µl IgG-sepharose 6 FAST Flow beads (GE Healthcare) for 1 h on a rotator at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted protein was separated on a 5 ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), and eluted with 2 mM desthiobiotin ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Neuroscience 2019Quote: ... Each probe contains a 3’-end amine-modified nucleotide that allows directly couple to an NHS-ester Cy3 dye (GE Healthcare, PA23001) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... was used in the experiments shown in figure 3 and supplementary figure 6C while a pool of four scrambled siRNAs (#D-001206-13-05, GE Healthcare Dharmacon) was used in the experiments shown in figure 2 ...
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bioRxiv - Cancer Biology 2020Quote: DNA probes complementary to the TP53 coding region or the 3′UTR were labeled with dCTP [α-32P] using the Amersham Megaprime DNA labeling system (GE Healthcare). Primers used for probe synthesis from human cDNA are listed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... a selection of mice were injected intra-peritoneally with 3 rounds of 6 injections each of 200μg poly(I:C) (GE Healthcare, #27-4732-01). Poly(I:C ...
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bioRxiv - Neuroscience 2020Quote: The blots were subsequently washed 3 x 5 min in TBST 1X and developed with the Prime Western Blotting detection Reagent (GE Healthcare Amersham) using the LAS3000 Bioimager (Fujifilm ...
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bioRxiv - Cancer Biology 2019Quote: ... was added to sample tubes and allowed to sit at room temperature for 3 minutes before quenching with 1.0 mL DMEM (GE Life Sciences, SH30081.01) + 6% FBS (Omega Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were then incubated with anti-BrdU (BU-1; 1:100; GE Healthcare, RPN202) and phospho-histone H2AX (Ser139 ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...