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Citations for GE Life Sciences :
2651 - 2700 of 4416 citations for Dengue Virus Serotype 3 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 5-10 μg denaturated proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Antibodies against HOIL1 (H-1 ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Cy5-labelled proteins were purified by size-exclusion chromatography (SEC) on a Superdex 200 Increase (GE Healthcare) column with PBS and concentrated on a 30 kDa Amicon Tube ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Labelling of the proteins was verified by measuring OD633 during SEC on the Akta Explorer (GE Healthcare). The final concentration was determined by OD280 measurement on the Nanodrop and verified by SDS-PAGE.
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bioRxiv - Immunology 2021Quote: ... The eluted protein was further purified using a Superdex 200 Increase 10/300 GL column (GE Healthcare). The purity and size of the protein were analyzed by sulphate-polyacryl-amide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was purified from cleared cell lysate using Ni-sepharose 6 Fast flow resin (GE Healthcare) in a poly-prep column (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated in centrifugal concentrators and purified by size-exclusion chromatography (Superdex 200; GE Life Sciences) in gel filtration buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... and protein bands were visualised via chemiluminescence using an ECL Plus Western blotting detection kit (GE Healthcare) and an LAS-4000 imager (Fujifilm) ...
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bioRxiv - Biophysics 2020Quote: ... The concentrated protein sample was loaded on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with GF buffer (50 mM TRIS pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... The protein conjugate was purified on a Superdex 200 increase 10/30 gL column (GE Healthcare, US) with a flow rate of 0.5 mL/min with PBS as elution buffer ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... Proteins were further purified by gel filtration chromatography (Superdex-75) using AKTA pure FPLC system (GE Healthcare) with a buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was then concentrated and loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... The proteins on the gel were transferred to a 0.2 μm pore size nitrocellulose membrane (GE Healthcare) for 10 min using a Power Blotter system (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted protein was concentrated (Amicon, 10kDa) and separated on a Superdex 75 10/300 column (GE Healthcare) equilibrated with SEC buffer 2 ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were then subjected to size-exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were further purified by size-exclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare) column ...
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bioRxiv - Cell Biology 2022Quote: ... Protein bands were visualized with ECL™ Prime Western Blotting detection reagents (GE Healthcare Life Sciences, RPN2236) and the pictures were taken with Bio-Rad ChemiDoc™ MP Imaging System.
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bioRxiv - Cell Biology 2022Quote: ... and then combined with 60 μl of washed Protein A–Sepharose beads (17-0780-01, GE Healthcare) for 1 hour on a rotator at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing GST-protein were pooled and loaded to a 6 mL Resource S column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Genetics 2022Quote: ... and proteins were transferred onto a membrane (Nitrocellulose Blotting Membrane 10600002; GE Healthcare Life Sciences; PA; USA). Primary antibodies were incubated overnight at 4°C and included mouse anti-3OH-K (1:1000 ...
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bioRxiv - Genetics 2022Quote: ... Separated proteins were then wet transferred to a 0.45 μm nitrocellulose membrane (GE Healthcare Life Science, 10600003) in Towbin buffer (25mM Trizma base ...
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bioRxiv - Immunology 2022Quote: ... MBs were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare, Chicago, IL) and eluted with 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Imidazole was removed by passing the eluted protein fraction over a desalting NAPTM-25 column (GE Healthcare) equilibrated with buffer A and eluted with 2.5 ml of the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... supernatant containing VSG protein was loaded onto an anion-exchange column (Q-Sepharose Fast-Flow, GE Healthcare), which had been equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion column (GE Healthcare) pre-equilibrated at 4 °C in SEC buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant protein GST-LSH10 was purified using Glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... The proteins were visualized by a chemiluminescence method (Amersham Imager 600, GE healthcare Life Sciences, Buckinghamshire, UK). Protein signals were quantified using ImageJ software (National Institute of Health ...
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bioRxiv - Plant Biology 2023Quote: ... Two mg of proteins in buffer were mixed with 50ul 7-Methyl-GTP-Sepharose 4B (GE Healthcare) previously equilibrated with extraction buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The cleaved proteins were separated by a 16/600 Superdex75 gel filtration column (GE Healthcare, Chicago, lL), followed by depleting the peak of lgMPZ peak or residual MBP by by applying to amylose resin ...
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bioRxiv - Biophysics 2022Quote: ... The dialyzed protein solution (∼50 μM) was then loaded into the sample cell in iTC200 (GE Healthcare) after the removal of insoluble materials by centrifugation (10,000×g ...
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bioRxiv - Cell Biology 2022Quote: ... cleared protein was subjected to size-exclusion chromatography using a HiLoad 16/600 Superdex column (GE Healthcare) in lysis buffer ...
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bioRxiv - Microbiology 2022Quote: Total IgG was purified from the final bleed rabbit sera using Protein G Gravitrap (GE Healthcare, Cytiva). The sera were diluted as 1:5 in binding buffer (20 mM Sod ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated on a 12% SDS-PAGE gel and transferred to a nitrocellulose membrane (GE Healthcare), which was then inucbated over-night with primary antibodies followed by a 1-hour incubation with fluorescent secondary antibodies (Table 1) ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins of interest were detected using Chemiluminescence by adding Amersham Enhanced chemiluminescence (ECL) detection reagent (GE healthcare). For quantification of Yops-secretion ...
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bioRxiv - Microbiology 2024Quote: Recombinant WT and mutant IN proteins were analyzed using Superdex 200 10/300 GL column (GE Healthcare) with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified from soluble fractions of the bacterial cell lysates using Glutathione Sepharose beads (GE Healthcare). Proteins were dialyzed in 1x TBS overnight at 4°C overnight and protein quantified ...
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bioRxiv - Cell Biology 2024Quote: ... and guinea pig anti-Rad21 and guinea pig anti-Pds5b coupled to protein A sepharose (GE Healthcare). For non-covalent coupling of antibodies to beads ...
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bioRxiv - Molecular Biology 2024Quote: ... The recovered proteins after cleavage underwent size exclusion chromatography using a Superdex S75/300 column (GE Healthcare) in phosphate-based buffer [200 mM sodium phosphate pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.1LmM PMSF) and included 80 pmol of GST-fused protein immobilized on GSH beads (GE Healthcare), 1–8 µg of heparin sulfate ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed using the described antibodies and protein G- or A- coupled Sepharose beads (GE Healthcare). Beads were washed with high salt buffer (50 mM Tris/Cl ...
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bioRxiv - Microbiology 2023Quote: Protein samples were separated on 10–15% polyacrylamide gels and transferred to Amersham nitrocellulose membranes (GE healthcare) by Trans-blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by SEC using a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated with Buffer B ...
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bioRxiv - Biochemistry 2022Quote: ... Protein purity was assessed by SDS-polyacrylamide gel electrophoresis (PhastGel Homogenous 20 pre-cast gels, GE Healthcare) and the enzyme concentration ([E] ...
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bioRxiv - Biochemistry 2022Quote: ... For crystallography proteins were purified further via size-exclusion chromatography (HiLoad 16/600 Superdex 200, GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were pre-incubated with 60 uL of protein A-Agarose beads (GE Healthcare, Chicago, USA) + Salmon Sperm DNA (Invitrogen #15632-011 ...
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bioRxiv - Microbiology 2022Quote: Proteins were purified by immobilized metal affinity chromatography (IMAC) using Ni-Sepharose excel resin (GE Healthcare, USA). The column was first washed with 5 column volumes of loading buffer (50 mM tris(hydroxymethyl)aminomethane (TRIS) ...
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bioRxiv - Microbiology 2022Quote: ... cell culture media were clarified by centrifugation and the IgG captured using Protein A resin (GE Healthcare). Proteins were eluted from the resin using citrate buffer pH 3 ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed proteins were concentrated and purified by SEC (HiLoad Superdex-200 16/600 column, GE Healthcare) into tau buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted proteins were concentrated and loaded onto a Superdex 200 column (10/300 GL; GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... wet electroblotting (100V for 1h) was used to transfer proteins onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...