Labshake search
Citations for GE Life Sciences :
2551 - 2600 of 4416 citations for Dengue Virus Serotype 3 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... the modified proteins were purified and separated from unbound dye through a gel filtration column (GE Healthcare illustra NAP Columns from Fisher ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified by size exclusion chromatography using Superdex 200 16/60 column (GE Healthcare) equilibrated in buffer containing 25mM Tris-HCl pH8.0 ...
-
bioRxiv - Cell Biology 2022Quote: Samples were separated on SDS-polyacrylamide gel and proteins were transferred onto a nitrocellulose membrane (GE healthcare) using a wet transfer system (BioRad) ...
-
bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
-
bioRxiv - Immunology 2020Quote: ... the supernatants with the secretion of antibodies were collected and captured by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and dialyzed into phosphate-buffered saline (PBS) ...
-
bioRxiv - Biochemistry 2021Quote: ... and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare), followed by a TEV protease cleavage at 16°C overnight ...
-
bioRxiv - Molecular Biology 2019Quote: ... The proteins were further purified by size exclusion chromatography on a Superdex200 16/60 column (GE Healthcare).
-
bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...
-
bioRxiv - Immunology 2021Quote: ... Rhesus sera or plasma antibodies were first purified by affinity chromatography using Protein A Sepharose (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The approximate molecular weights of the loaded proteins were determined by molecular weight calibration kits (GE Healthcare).
-
bioRxiv - Molecular Biology 2020Quote: ... The GST part was cleaved off from the recombinant protein using PreScission Protease (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... the protein was concentrated and further purified by gel filtration using a superpose 6 increase (GE healthcare) in TBS containing 0.01% LMNG ...
-
bioRxiv - Bioengineering 2021Quote: ... The protein was further purified by size-exclusion chromatography using a HiLoad Superdex 200PG column (GE Healthcare) with PBS as an eluent ...
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were purified by immobilized metal-affinity chromatography (IMAC) using pre-packed nickel Sepharose columns (GE Healthcare), or in batch mode on TALON® beads (Takara Bio ...
-
bioRxiv - Immunology 2020Quote: ... and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare) equilibrated with TBS ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved by 10% SDS PAGE and transferred on a nitrocellulose membrane (Protran BA83, GE Healthcare) using a semi-dry system (Bio-Rad Laboratories S.r.l.) ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were either stained with Coomassie brilliant blue G-250 or transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in 0.05% Tween TBS at room temperature for 1 h and then incubated at 4 °C overnight with a specific polyclonal serum produced by immunization of mice with RBD produced in HEK-293T cells ...
-
bioRxiv - Biochemistry 2020Quote: ... the proteins were buffer exchanged into the appropriate buffer using an Illustra NAP-5 column (GE Healthcare).
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were then loaded on a Superdex 200 10/300 GL Tricorn gel filtration column (GE Healthcare), and eluted with 1.5 column volumes of gel filtration buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biophysics 2020Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 250 µl of antiserum were coupled to 60 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed three times in CPB and supplemented with 6 µl fresh rPAS ...
-
bioRxiv - Molecular Biology 2022Quote: ... Concentrated protein was loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare Piscataway, NJ). The buffer conditions were the same as in Ni2+-NTA affinity chromatography but with 20 mM NaCl and no imidazole ...
-
bioRxiv - Neuroscience 2022Quote: ... The Twin-Strep-tagged protein was purified via affinity chromatography using a StrepTrap HP column (GE Healthcare). Next ...
-
bioRxiv - Microbiology 2022Quote: ... Periplasmic proteins were extracted and CusF was purified on a 5 ml StrepTrap HP column (GE healthcare) equilibrated with buffer A (10 mM NaPi ...
-
bioRxiv - Microbiology 2022Quote: ... proteins were eluted and further purified by size-exclusion chromatography on Superdex 75 (GE Healthcare Life Sciences). Purified DksA proteins were aliquoted inside a BACTRON anaerobic chamber (Shel Lab ...
-
bioRxiv - Biochemistry 2022Quote: ... thrombin or PreScission protease and proteins were further purified by ion exchange chromatography (Source 15Q, GE Healthcare) and gel filtration chromatography (Superdex 200 ...
-
bioRxiv - Microbiology 2019Quote: ... Untagged Orn proteins were recovered in the flow-through of a HisTrap Ni-NTA column (GE Healthcare), separated from His6-SUMO ...
-
bioRxiv - Pathology 2020Quote: ... The presence of P1 proteins was revealed using the ECL Plus Western Blotting Detection System (GE Healthcare).
-
bioRxiv - Biophysics 2019Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2019Quote: ... where the concentrated protein sample was applied onto a Superose 6 10/300 GL column (GE Healthcare) equilibrated with SEC buffer (25 mM Tris-HCl pH 8 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Proteins were visualized using Amersham ECL Prime Western Blotting Detection System and Amersham Hyperfilm ECL (GE Healthcare).
-
bioRxiv - Biochemistry 2019Quote: ... resin and excess biotin was separated from the labeled protein by Superdex75 gel filtration chromatography (GE Healthcare) in 20 mM HEPES buffer pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: ... the holo-proteins were then loaded onto a Superdex 75 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with buffer A ...
-
bioRxiv - Cancer Biology 2019Quote: ... The proteins were then transferred to a 0.45-μm PVDF membrane (Amersham Hybond, GE Healthcare, München, Germany) which was blocked in 2% bovine serum albumin (Amresco ...
-
bioRxiv - Molecular Biology 2019Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2019Quote: ... a frozen protein aliquot was thawed and loaded onto a Superdex HR 75 10/300 (GE Healthcare) size exclusion column and eluted with 150 mM NaCl ...
-
Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infectionbioRxiv - Cell Biology 2020Quote: ... proteins were further concentrated with filters and then purified by anion exchange chromatography on HitrapQ (GE Healthcare) and collected fractions were further loaded onto size exclusion column (Superdex 75 16/60 ...
-
bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
-
bioRxiv - Microbiology 2019Quote: ... Dual-labeled capsid proteins were separated using a PD Mini-Trap G-25 desalting column (GE healthcare) with imaging buffer (50 mM Tris ...
-
bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare, Mississauga, ON). Endotoxin was removed using the Pierce™ High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
-
bioRxiv - Molecular Biology 2021Quote: ... Size-exclusion chromatography was performed by loading the protein onto Superose 6 Increase 10/300GL (GE Healthcare) column with running buffer containing 20 mM HEPES ...
-
bioRxiv - Plant Biology 2021Quote: ... The supernatant containing soluble proteins was collected and allowed to flow through Ni-NTA resin (GE Healthcare). After washing with three column volumes of sonification buffer ...
-
bioRxiv - Plant Biology 2020Quote: ... The cleaved protein was purified further using a Superdex Increase 75 10/300 (GE Healthcare Life Sciences), pre-equilibrated with 20 mM Hepes pH 7.5 and 150 mM NaCl.
-
bioRxiv - Biophysics 2020Quote: ... The plexin protein in the supernatant was captured using a 1 mL HisTrap FF column (GE Healthcare) and eluted by Buffer B containing 10 mM Tris (pH 8.0) ...
-
bioRxiv - Plant Biology 2020Quote: The GST- and MBP-fusion proteins were separately purified using Glutathione sepharose (GE Healthcare, 17-5132-01) and Amylose resin (New England Biolabs ...
-
bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-tagged proteins were immobilized on 100 μl Glutathione–Sepharose beads in 1xPBS (GE Healthcare, Freiburg, Germany) directly from E ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein was buffer exchanged to remove imidazole using a 16/260 HiLoad desalting column (GE healthcare). BpsDsbA was then incubated with TEV protease in a 1:50 (TEV:BpsDsbA ...
-
bioRxiv - Plant Biology 2021Quote: ... Recombinant protein MdSUMO2A-HIS and MdDREB2A were purified by HIS Sepharose beads (GE Healthcare, Fairfield, CT, USA), GST-MdCE was purfied by Pierce™ Glutathione Spin Columns (16105 ...