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Citations for GE Life Sciences :
2901 - 2950 of 4416 citations for Dengue Virus Serotype 3 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the two proteins were mixed together in solution and purified using a Superdex200 gel filtration column (GE Healthcare). The complex was concentrated to 10 mg/ml in buffer 20 mM Tris pH 7.2 and 200 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins from the cleared cell lysate were captured in a HisTrap HP 5 ml column (GE Healthcare) in His buffers (20 mM MOPS ...
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bioRxiv - Pathology 2020Quote: ... wt:wt) was determined by size exclusion chromatography in AKTA Primer Plus fast protein liquid chromatography system (GE Healthcare) with Superose™ 6 10/30 GL Column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare). Antibodies were eluted using 0.1M glycine pH 2 ...
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bioRxiv - Biochemistry 2020Quote: ... This protein was applied to a 16/600 Superdex 75 preparative size exclusion column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A ...
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bioRxiv - Immunology 2019Quote: ... The VLP recombinant proteins were further purified by size exclusion chromotogrophy (Superose 6, 10/300 GL;GE Healthcare). The level of endotoxin in all the purified VLPs was measured with an ELISA-based endotoxin detection assay (Hyglos ...
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bioRxiv - Biochemistry 2021Quote: ... Target protein was further purified by passage through a HiLoad 16/600 Superdex 75 pg (GE Healthcare, USA) in buffer E (20 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... The proteins were detected using the Amersham ECL Prime Western Blotting Detection Reagent (RPN2232, GE Healthcare Life Sciences) and visualized on the ECL Chemocam Imager (INTAS) ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare), and purity was confirmed to be at least 90% by SDS polyacrylamide gel electrophoresis ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was loaded onto a HiLoad 26/60 Superdex 200 prep grade column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated and further purified using an S200 16/60 gel filtration chromatography column (GE Healthcare) previously equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein bands were detected with the enhanced chemiluminescence method (FUJIFILM Wako) and chemiluminescence film (GE Healthcare, Buckinghamshire, UK), and target protein levels were analyzed using NIH ImageJ software.
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bioRxiv - Microbiology 2020Quote: ... protein bands were visualized by chemiluminescence and the image was captured by ImageQuant LAS 4000 mini (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... Protein samples were resolved on the SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare). The membrane was blocked with 5% non-fat dry milk in tris-buffered saline ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Proteins of interest were visualized on a Bio-Rad ChemiDoc MP imager with Enhanced Chemiluminescence Plus (GE Healthcare) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: Cell lysates (10μg protein) were resolved by electrophoresis and transferred to a polyvinylidene fluoride membrane (GE Healthcare, UK). Membranes were blocked for 1 hour in 5% skimmed milk/TBS/0.1% Tween-20 and incubated with primary antibodies (1:1000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Protein was further purified by size exclusion over a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) in 100 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins were purified directly from the filtered culture supernatants using HisTrap Excel columns (GE Healthcare Life Sciences). Each column was stored in 20% ethanol and washed with 5 column volumes (CV ...
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bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
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bioRxiv - Microbiology 2021Quote: ... The protein extracts were separated by SDS-PAGE and were subsequently transferred to Amersham Protran membranes (GE Healthcare) in a tankblot device (BioRad ...
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bioRxiv - Molecular Biology 2019Quote: ... The absorbance of fluorescently labeled proteins was followed during chromatography using the AKTAmicro Monitor UV-900 (GE Healthcare) and by SDS-PAGE using a ChemiDoc MP system (Bio-Rad).
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bioRxiv - Plant Biology 2019Quote: ... The protein was then further purified by further purified by gel filtration (Superose 6, 10/30; GE Healthcare). For cryo-EM investigation ...
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bioRxiv - Molecular Biology 2019Quote: ... The concentrated protein sample was then applied to Superdex 200 Increase 10/300 GL Increase column (GE Healthcare) equilibrated with buffer containing 25 mM HEPES-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell-free synthesized N11-tagged hTERT_191-306 protein was purified using an AKTA 10S system (GE Healthcare) with a HisTrap columns (GE Healthcare) ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting tag-cleaved proteins were purified by ion exchange chromatography with a HiTrap SP column (GE Healthcare) and gel filtration with the final buffer (25 mM Tris-HCl buffer at pH 7.0 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then purified by anion exchange chromatography on a MonoQ 5/50 GL column (GE Healthcare) using buffers AEX-A and AEX-B with a gradient of 7.5-50% B over 20 CV at a flow rate of 1 ml/min ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of proteins were resolved in Tris-acetate SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). For extracellular vesicle fractions ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 40 mM HEPES pH 7.4 (4 °C) ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The excess dye was separated from the labelled protein by SEC (Superdex 75 10/300 GL, GE Healthcare) performed in SEC buffer D (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tag free Ape1 protein was then loaded onto a Superdex 200 10/300 GL column (GE Healthcare) in 20 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... cells according to the high titer protocol provided and purified on a Protein A HiTrap column (GE Healthcare) with the ACTA Pure FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... the bromodomain was purified further using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare), equipped with a HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare, USA) and purified using Assay Buffer or Assay Buffer without EDTA (for use in DNA cleavage assays) ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified using a Superdex Increase 10/300 GL column (GE Healthcare, Chicago, IL) equilibrated with buffer containing 350 mM NaBr ...
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bioRxiv - Cancer Biology 2021Quote: ... We subjected equal amounts of proteins to SDS-PAGE and transferred them to nitrocellulose membranes (GE Healthcare #10401197). Non-specific binding was blocked by incubation with TBST plus 5% non-fat milk ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was loaded on a HiPrep Q FF Anion Exchange Column (GE Healthcare Life Sciences, Marlborough, MA) and eluted by buffer with 300 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was loaded onto a His-Trap column using an ÄKTA protein purification system (GE Healthcare Life Sciences). The chromatography was run with a two-step gradient started with 100% starting buffer (500 mM sodium chloride ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were sonicated and His-tagged proteins were purified using affinity chromatography (HisGraviTrap column from GE Healthcare).
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Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: SEC-MALS analysis of the purified proteins was performed on Superdex 75 10/300 GL column (GE Healthcare) using an Agilent HPLC system equipped with the 18-angle light scattering detector (Wyatt Dawn HELIOS II ...
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bioRxiv - Microbiology 2021Quote: ... The protein complex was then purified by SEC using a Superdex 200 Increase 10/300 GL (GE Healthcare) column ...
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bioRxiv - Cell Biology 2019Quote: ... for the detection of ubiquitinated protein aggregates or a 0.2 μm pore size cellulose acetate membrane (GE Healthcare) for the detection of huntingtin aggregates using a 96 well format Dot-Blot apparatus (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Immunology 2020Quote: ... we used fritted deep well plates containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...