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Citations for GE Life Sciences :
2451 - 2500 of 3164 citations for Dechlorane Plus Syn Bis Cyclopentene 13C10 99% 100 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... concentrated with Amicon Millipore 15 ml 100,000 MWCO centrifugal concentrator and applied to Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated in storage buffer (400M KCl ...
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bioRxiv - Biochemistry 2019Quote: ... to 3 ml and the protein further purified by size exclusion chromatography (Hiload 16/600 Superdex 200 pg, GE healthcare) equilibrated with SEC buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... concentrated to a volume of 2 ml and applied onto a S75 16/600 pg size exclusion column (GE Healthcare), equilibrated with 10 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2019Quote: ... and buffer exchanged to 10 mM phosphate buffer pH 7.4 containing 10 mM MgCl2 using 10 kD Vivaspin 20 ml columns (GE Healthcare). Samples were then loaded onto a CHT ceramic hydroxyapatite column (Bio-Rad Laboratories Inc.) ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing the periplasmic extract was sonicated before being loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with stepwise increases of imidazole in buffer E (50 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... then supernatants were filtered (0.45 μm syringe filter, Iwaki) prior to purification on 5 ml HiTrap Chelating HP columns (GE Healthcare). Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC ...
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bioRxiv - Cell Biology 2019Quote: ... The sample was concentrated on an Amicon Ultra Centrifugal Filter Unit (10 kDa cut-off) and injected at 0.5 ml/min on a Hi-Load 16/60 Superdex column (GE healthcare), equilibrated with 20 mM Tris-HCl buffer ...
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bioRxiv - Microbiology 2019Quote: ... 2.5 ml of fractions containing the highest protein content were pooled and the imidazole was removed by running the 2.5 ml protein suspension on PD-10 size-exclusion columns (GE Healthcare) followed by cold 3.5 ml of Buffer A to elute proteins ...
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bioRxiv - Biophysics 2019Quote: ... The final step was a size exclusion chromatography in a hand-made 275 mL Superdex 75 Column (Dimensions 2.50 × 60 cm, GE Healthcare). ARD concentration was determined using a theoretical extinction coefficient of 1.21 L/mg·cm obtained using the ProtParam Expasy WebServer (34 ...
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bioRxiv - Molecular Biology 2019Quote: ... and those containing Tdp1 were pooled and concentrated to ~0.5 mL by binding to a small volume of SP sepharose resin (GE Healthcare) and elution with 50% buffer B ...
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bioRxiv - Biochemistry 2021Quote: ... The buffered supernatant was loaded on a DEAE-20 mL HiPrep FF 16/10 anion exchange chromatography column (GE Healthcare), equilibrated with Tris-HCl buffer (50 mM ...
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bioRxiv - Biochemistry 2020Quote: ... The obtained material was concentrated to reach a final volume ≤0.5 mL and further purified by SEC on a Superdex 200 10/300 Increase column (GE Healthcare), equilibrated with buffer GF (200 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... A final polishing step was performed by size exclusion chromatography (SEC) on a 24 mL Superdex 200 increase (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... the lysate spun down at 12,000 x g for 30 min and then loaded onto a 5 ml His-Trap Crude FF column (GE Healthcare) using a ÄKTA Purifier protein purification system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 35,000g for 20 min and the supernatant from 1 l of cell culture was incubated with 2.5 ml of Glutathione Sepharose beads (GE Healthcare) at 4°C for 6 h The Glutathione Sepharose beads were then collected in a PD10 column and washed with 50 ml of PBS ...
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bioRxiv - Microbiology 2021Quote: ... The resulting lysate was centrifuged for 30 minutes at 20,000 rpm to remove cell debris and the supernatant was filtered with a 0.45 μm filter before loading onto an equilibrated 5 ml His Trap HP column (GE healthcare) connected to an ÄKTA pure 25 system ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was collected and incubated with 2 column volume (for 20 mL supernatant) of Gluthatione Sepharose 4B (GE Healthcare) for 1h30 on a rotating wheel ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2020Quote: ... for 30 minutes with or without cofactor GMPPNP (30 μM) then applying 0.5 mL to a PD-10 Sephadex G-25 gel-filtration column (GE Healthcare). Eluted fractions (0.5 mL ...
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bioRxiv - Biochemistry 2021Quote: ... The periplasmic extract was adjusted to 50 mM Tris/HCl pH 9.0 (loading buffer) and loaded onto a 5 mL Q Sepharose anion exchange column (GE Healthcare). Proteins were eluted using a linear salt gradient (0-500 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: The sample was desalted into Buffer A using a HiPrep 53 ml 26/10 desalting column (GE Healthcare, Chicago, IL) with 14 ml injections at 9 ml/min for all steps ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting eluate was manually applied at room temperature to a 1 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant containing the protein was loaded on a 5 mL HiTrap Ni-chelating column (GE Healthcare, Piscataway, NJ), washed ...
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bioRxiv - Biochemistry 2020Quote: ... Medium was collected 48-60 h post infection and incubated with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) pre-equilibrated with Buffer A (150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Pooled eluate fractions were adjusted to 150 mM NaCl and purified on a 5 ml HiTrap Heparin column (GE Healthcare) using a linear gradient from 150 mM NaCl to 1 M NaCl in buffer A ...
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bioRxiv - Biophysics 2021Quote: ... nickel affinity chromatography was performed where the lysate was loaded onto a 5 mL HisTrap™ Excel column (GE Healthcare), followed by a wash with 20 column volumes (CV ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: Single cysteine mutants of ColE9 or the ColE9 DNase domain were desalted on a 5 ml HiTrap column (GE Healthcare) equilibrated with binding buffer (20 mM potassium phosphate pH 7 ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble cell lysate obtained after centrifugation was loaded onto a 5 ml HisTrap FF HP nickel column (GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The co-expressed complex of ctCyclin H and ctMAT1short was loaded on a 5 ml StrepTrap HP column (GE Healthcare) equilibrated in lysis buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was centrifuged at 38,000 ×g and the supernatant was loaded onto a 5 mL HisTrap Ni-NTA column (GE Healthcare). The column was washed with HisTrap Buffer and protein eluted with a linear gradient of imidazole from 25 to 300 mM in HisTrap Buffer over 10 column volumes.
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bioRxiv - Systems Biology 2021Quote: Biomass dry weight determination was done as previously described [105] by filtering 10 mL of culture on a filter with pore-size 0.45 mm (Whatman/GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... then 1.5 column volume of mobile phase (DPBS) was run through the column at flow rate of 0.5 mL/min using the AKTA Pure (GE Healthcare) system ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatants obtained after this step were loaded in 5-mL His-trap columns (GE Healthcare Systems, Chicago, IL, USA) pre-equilibrated with buffer A supplemented with 100 mM NaCl and 20 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... the obtained supernatants were filtered through sterile PVDF filters (pore size 0.22 μm) and loaded on 1 ml His-Trap columns (GE Healthcare), pre-equilibrated with ten column volumes of each Millipore water and sodium phosphate buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was filtrated through a O.22μm pore filter and loaded on a 5 ml pre-packed Hitrap Q FF column (GE Healthcare) equilibrated with buffer A (50mM bis-tris-HCI pH 6.0 ...
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bioRxiv - Neuroscience 2019Quote: ... with 2% FBS and 0.5 mg/mL DNase I) and endothelial cells were obtained using density gradient centrifugation with 22% Percoll (GE Healthcare) in PBS (1X) ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane extract was centrifuged for 35 mins at 200,000 x g and the supernatant loaded onto a 10 ml Nickel column (Chelating Sepharose; GE Healthcare) equilibrated in TSB with 0.2% DDM pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was subjected to immobilized-metal affinity chromatography (IMAC) using a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB1 containing 20 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation for 30 min at 47,000 xg and the supernatant was loaded onto a 5 ml GSTrap column (GE Healthcare). The resin was washed by the lysis buffer and bound protein was eluted with elution buffer of 100 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Human IgGs were isolated from heat-inactivated plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) as described (24).
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bioRxiv - Microbiology 2020Quote: ... The clarified lysate was then passed using an ÄKTA Purifier FPLC through a 1 ml HisTrap HP column (GE Healthcare), pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... then immediately rebuffered in 3.5 mL assay buffer (sodium citrate buffer 50 mM, pH 6) in a PD-10 gel filtration column (GE Healthcare) and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was cleared by centrifugation and it was applied to a 5 ml GSTrap FF column (GE Healthcare, 17513101) to purify GST-proteins ...
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bioRxiv - Cell Biology 2021Quote: ... with a JA30.50 Ti rotor at 20,000 ×g and applied to a HiTrap SP HP column (GE Healthcare; 1 ml) equilibrated in buffer A (20 mM Na-phosphate buffer (pH 7.0) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with a 20 ml linear gradient from 150 mM to 500 mM at a flow rate of 0.5 ml/min using an ÄKTA Pure instrument (GE Healthcare). The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore ...