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Citations for GE Life Sciences :
2351 - 2400 of 4416 citations for Dengue Virus Serotype 3 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... The proteins were then transferred from the gel to the PVDF membrane (GE Healthcare Life-Science) in Bio-Rad vertical gel Transfer Apparatus using Transfer buffer (2.5mM Tris base ...
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bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... Biotinylated proteins were purified using SEC on a Superdex 200 10/300 Increase GL (GE Healthcare) or S75 10/300 Increase GL (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were further purified by gel filtration on Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein detection was finally performed using Amersham ECL Western Blotting detection reagent (GE Healthcare #CA95038-564L).
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bioRxiv - Immunology 2023Quote: ... The proteins were visualized by ECL-Plus (GEHealthcare) using Image Quant LAS500 (GE Healthcare Life Sciences). The following antibodies were used ...
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bioRxiv - Plant Biology 2023Quote: Protein samples were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). After being blocked in 1 × PBST buffer containing 5% skimmed milk ...
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bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... 25µL of Protein L beads (Cat. no. Capto™ L resin, GE Healthcare Cat. no. 17547802), pre-equilibrated with binding buffer (20mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... the protein was further purified by size-exclusion chromatography on a Sephacryl 300 column (GE Healthcare) in PBS.
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bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... A small portion of the final proteins were run through the Superdex 200 columns (GE Healthcare) again for quality check.
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bioRxiv - Genomics 2023Quote: ... 167 mM NaCl) and pre-cleared with protein-A-Sepharose beads (GE Healthcare 17-5280-02) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Samples were pre-cleared with 40 µl of protein G Sepharose beads (50% slurry, GE Healthcare) plus 5 μg Goat IgG (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare) were performed according to our published procedure (Ninagawa et al. ...
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bioRxiv - Biophysics 2023Quote: ... The protein was injected onto a Superdex-75 Increase 10/300 GL column (GE Healthcare 29148721) for size exclusion chromatography in SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Free copper was then removed by passing the protein through a HiTrap Desalting column (GE Healthcare). Prior to the SANS experiments ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was loaded onto a HiLoad™ 16/600 Superdex™ 200 column (GE Healthcare) equilibrated with a buffer containing 30 mM Tris-HCl pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... All proteins were further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... then further purified using Gammabind Plus Sepharose Protein G 25 mL column (GE Healthcare, Life Sciences), with 20 min contact time ...
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bioRxiv - Biochemistry 2024Quote: ... Separation of the labeled protein from residual dye was carried out using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Scaffolds and RSVF trimer or IgG proteins were immobilized on a CM5 chip (GE Healthcare # 29104988) by amine coupling ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred overnight onto nitrocellulose membranes (Whatman, GE Healthcare Life Sciences, Buckinghamshire, UK) in a 1 litre tank containing transfer buffer at 30 V.
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bioRxiv - Cancer Biology 2024Quote: ... mixed with 25 μl of protein A-Sepharose beads (GE healthcare, catalog no. 17-0469-01). The immunoprecipitated proteins were eluted from protein A-Sepharose beads by boiling with 2x sample buffer and subjected to SDS-PAGE.
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bioRxiv - Biochemistry 2024Quote: Anti-ASCT2 Ab was purified from the anti-sera using HiTrap Protein G HP (GE Healthcare) following the manufacturer’s protocol ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing the target protein were pooled and loaded onto a Superdex S75 column (GE Healthcare) equilibrated in buffer containing 150 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein for crystallization was further purified by size-exclusion chromatography on a Superdex 75 (GE Healthcare) with gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... The protein extract was precleared by incubation with nProtein A-Sepharose 4 Fast Flow (GE Healthcare) for 30 min and then incubated with 20 uL of rabbit anti-Myc antibody (ab9106 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected with enhanced chemiluminescence (ECL) detection reagents (RPN2236 or RPN2235, Amersham Biosciences, GE Healthcare,) and ChemiDoc™ XRS+ System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were separated by SDS-PAGE and transferred onto 0.45μm pore Protran nitrocellulose membrane (GE Healthcare) using semi-dry transfer apparatus (Hoeffer) ...
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bioRxiv - Biochemistry 2024Quote: ... Then tag-cleaved proteins was purified with ion exchange chromatography (HiTrap SP FF column (GE Healthcare). Target proteins in the HiTrap SP FF column were eluted by a linear gradient of NaCl and further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Physiology 2024Quote: ... Target proteins were visualized by enhanced chemiluminescence using a commercially available kit (GE Healthcare, Freiburg, Germany).
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bioRxiv - Plant Biology 2024Quote: ... The bound GST-COI1 protein was detected using an anti-GST HRP conjugate (RPN1236, GE Healthcare, USA ...
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bioRxiv - Microbiology 2024Quote: ... The proteins separated on the gels were transferred to nitrocellulose membranes (Amershan Protan membranes, GE Healthcare) using transfer buffer (0.192 M Glycine ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with GST or GST-tagged angulin-1 cytoplasmic region (409-575 aa or 409-570 aa) for 2-3 h at 4°C and further incubated with Glutathione Sepharose 4B beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: 30 ml PBS flow-through plasmapheresis filters or peripheral blood diluted in PBS (1:3) were collected in 50 ml tubes and underlaid with Ficoll-Paque (GE Healthcare). PBMC-containing buffy coat was obtained after centrifugation for 30 min at room temperature at 1200 RCF ...
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bioRxiv - Biophysics 2022Quote: ... the volume was reduced to a volume of 400 ml using the ÄktaFlux system with a 3 kDa MWCO cartridge (GE Healthcare). Afterwards the system was used for buffer exchange with 50 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: Each oligonucleotide contained an amino group at the 3’ end for fluorophore coupling using either Cy5 NHS ester (GE Healthcare, PA15102) or rhodamine NHS ester (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... Protein containing fractions were concentrated to 3 mL and further purified by size exclusion using a HiPrep 16/60 Sephacryl S-500 HR (GE Healthcare) in Buffer D (20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The reaction took place for 3 h and the radio labeled kCR probe purified in a Sephadex G25 column (Amersham, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Purified 6-His-PI3Kγ catalytic subunit17,18 was used as the soluble analyte and was injected at different concentrations over the immobilized GRK2 in SPR buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% surfactant P-20, GE Healthcare Life Sciences, Biacore Inc.). Binding experiments were performed at 25°C in SPR buffer with a flow rate of 20 μl/min ...
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bioRxiv - Cancer Biology 2021Quote: ... Stained plates were washed 3 times in dH2O for 5 minutes at room temperature and air dried before imaging using the Imagescanner III (GE Healthcare). Growth was determined by percentage area of the plate covered by cells using ImageJ v1.52a ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Plant Biology 2020Quote: ... Each Z-section image is based on 15 images generated from 3 different angles and 5 different SIM patterns and reconstructed using SOFTWORX software (Applied Precision).