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Citations for GE Life Sciences :
201 - 250 of 1251 citations for Transmembrane Serine Protease 11A TMPRSS11A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved MBP tag and TEV protease were removed by anion exchange chromatography using a Mono Q 5/50GL (GE Healthcare) column pre-equilibrated with buffer-B ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cleaved protein was separated from TEV protease by a subtractive Ni-NTA pass and loaded on to a HiLoad 16/60 Superdex 75 column (GE Healthcare) equilibrated with 20 mM Hepes ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP and His tags were removed by TEV protease and a passage through a Ni2+chelating Sepharose (GE Healthcare). The final purification step was size-exclusion chromatography on Superdex 200 size exclusion chromatography (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... The GST tag was cleaved off by incubating the washed beads in wash buffer supplemented with PreScission Protease (∼100 mL at 76 mg/mL) (GE Healthcare) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The liberated affinity-tag and the His-tagged SENP1 and 3C proteases were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75pg ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP tag was cleaved off by TEV protease and the protein was further purified by a passage through a Ni2+-Chelating Sepharose (GE Healthcare). IHO1 was then purified by a gel filtration on Superdex 200 (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... followed by removal of the N-terminal His6-Ztag by TEV protease cleavage and size exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) (Roostalu et al ...
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with a linear gradient of increasing NaCl concentration from 300 mM to 1 M in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... the His6-NusA-tag was removed using the HRV3C protease and was further purified by SEC on Superdex 75 Increase 10/300 (GE Healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare) in TBS
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was digested with the TEV protease overnight and further purified by gel filtration chromatography using a Superose 6 column (GE Healthcare), eluted in buffer C (25 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The N-terminal GFP-His10 tag was cleaved overnight with 3C protease before gel filtration chromatography by using a Sepharose 6 Increase 10/300 GL column (GE Healthcare). For the purification of apo proteins ...
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bioRxiv - Biophysics 2024Quote: ... His6-GB1-tag was subsequently cleaved by TEV protease (3 mM) and separated from bZIP on a cation exchange column (HiTrap SP; GE Healthcare) using a 0–2 M gradient of sodium chloride ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies for WB were horseradish peroxidase-conjugated anti mouse (NA931) or anti rabbit (NA94V) antibodies from GE Healthcare. ECL chemiluminescent kit (RPN2132 ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-mouse antibody (sheep polyclonal, used at 1:3000, GE Healthcare), HRP-conjugated anti-rabbit antibody (sheep polyclonal ...
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bioRxiv - Cell Biology 2019Quote: ... with respective primary antibodies and visualized by HRP-conjugated secondary antibodies by enhanced chemiluminescence (GE Life Sciences, Buckinghamshire, England).
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bioRxiv - Neuroscience 2020Quote: ... The antibody-functionalized nanoparticles were separated from unbound antibodies using a Sepharose CL-4B (GE Healthcare, Little Chalfont, UK) size-exclusion chromatography column eluted with PBS (dimensions ...
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bioRxiv - Immunology 2021Quote: ... All primary antibody incubations were followed by incubation with appropriated secondary HRP-conjugated antibodies (GE Healthcare or Cell Signaling) in 5% milk plus 0.1% Tween20 (Sigma P2287) ...
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bioRxiv - Cell Biology 2019Quote: ... primary antibody and horseradish peroxidase linked anti-rabbit IgG (from donkey) secondary antibody (1:5000, GE Healthcare Life Sciences). Signal was detected by chemiluminescence (Luminata Forte Western HRP substrate ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was cleaved with Precission™ protease for 2 hours at room temperature and applied to a 5 mL HiTrap Q HP column (GE Healthcare). Protein was eluted from the Q HP column with a 2M NaCl buffer in a 100 mL gradient ...
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bioRxiv - Molecular Biology 2021Quote: ... hRpn13 Pru was eluted from the resin by overnight incubation with 50 units per mL of PreScission protease (GE Healthcare Life Sciences) in buffer B (20 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: ... The fusion protein was cleaved with 10% (w/w) TEV protease overnight at 4°C and the mixture was loaded on a 5 ml HisTrap™FF column (GE Healthcare). 6xHis-tag-labeled disulfide bond isomerase and TEV protease were retained ...
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bioRxiv - Cell Biology 2020Quote: ... 2004) primary antibody and horseradish peroxidase linked anti-rabbit IgG secondary antibody (1:5000, from donkey, GE Healthcare Life Sciences). Signal was detected by chemiluminescence (Luminata Forte Western HRP substrate ...
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bioRxiv - Bioengineering 2020Quote: ... for HRP-conjugated antibodies: donkey monoclonal anti-mouse (1:10000) and donkey monoclonal anti-rabbit antibody (1:10000, GE Healthcare) diluted in 2% BSA-TBST ...
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bioRxiv - Biophysics 2019Quote: ... chip was prepared by amine coupling of anti-mouse IgG antibodies at pH 5.0 (Mouse antibody capture kit, GE Healthcare) according to the protocol described by manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... anti-ClpP polyclonal antibody (62) and an anti-rabbit IgG antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, Code#: NA934) were used ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with the secondary antibody (HRP-conjugated anti-mouse or anti-rabbit IgG antibody, GE Healthcare; 1:2000) in TBST plus 2% BSA ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
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bioRxiv - Biophysics 2024Quote: ... The secondary antibodies used were a sheep α-mouse antibody conjugated to horseradish peroxidase (HRP) (GE Life sciences, Marlborough MA) at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences ...
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... or anti-mouse secondary antibody (GE Healthcare, NA931V). Proteins were visualized using Pico Plus chemiluminescence substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were anti-rabbit (NA934V, GE Healthcare) or anti-mouse (NA931V ...
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bioRxiv - Genomics 2019Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare) and ECL developing reagent (Amersham Biosciencies ...
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bioRxiv - Cell Biology 2019Quote: ... anti-mouse secondary antibody (1:4000, GE Healthcare) or alpaca anti-chicken (1:4000 ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies were anti-mouse-HRP (GE Healthcare) or anti-rabbit-HRP (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... Conjugated secondary antibodies against rabbit (GE Healthcare NA934) or guinea pig (Sigma AP108P ...
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bioRxiv - Molecular Biology 2019Quote: ... The secondary antibodies were HRP conjugated (GE Healthcare), and the blots were developed using an enhanced chemiluminescence method (ECL kit ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rat and anti-mouse antibodies (GE Healthcare) used at a dilution of 1:5,000 ...
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bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse (GE Healthcare) or HRP-conjugated anti-rabbit (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare) used were anti-mouse/rabbit (1:5,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase-conjugated antibodies were used (GE Healthcare) and data was developed using an Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with peroxidase-conjugated secondary antibodies (GE Healthcare) and visualised with chemiluminescent substrates (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... Peroxidase-conjugated secondary antibodies were from GE Healthcare. Alexa-conjugated secondary antibodies were from Life Technologies.
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bioRxiv - Neuroscience 2019Quote: ... Horseradish peroxidise (HRP)-conjugated secondary antibodies (GE Healthcare) were detected using enhanced chemiluminescence (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... with horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) and quantified using ImageJ.
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies conjugated with horseradish peroxidase (GE Healthcare) were used at 1:5000 for immunoblotting.