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Citations for GE Life Sciences :
51 - 100 of 1251 citations for Transmembrane Serine Protease 11A TMPRSS11A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... GST tags were cleaved with PreScission protease per the manufacturer’s instructions (GE Healthcare). Isolated CFP1 fragments were dialyzed in 25% glycerol ...
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bioRxiv - Molecular Biology 2019Quote: ... bead-coupled proteins were incubated with PreScission Protease (GE Healthcare, 27-0843-01) in reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was cleaved by the addition of PreScission Protease (GE Healthcare) with rocking incubation overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells are passaged following treatment with 0.25% trypsin protease (GE Healthcare Life Sciences) and counted using TC20 cell counter (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... the GST-tag was removed by proteolytic cleavage with PreScission Protease (GE Healthcare), followed by a final polishing step of the proteins by size-exclusion chromatography on an Äkta Purifier System using either HiLoad 26/600 Superdex 200 or HiLoad 26/75 Superdex columns (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The PreScission protease was removed by incubation with Glutathione Sepharose beads (GE Healthcare) at 4 °C for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... another 2 ml of buffer and 10 μl of PreScission protease (GE Healthcare) were added to the column followed by overnight incubation to cleave off the AVR proteins from the GST resin ...
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bioRxiv - Neuroscience 2020Quote: ... antibody with respective secondary antibodies (1:5000, GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... Uncleaved protein and protease were removed via reverse IMAC (1ml HisTRAP FF (GE Healthcare) followed by gel filtration (HiLoad® 16/600 Superdex® 75 pg (GE Healthcare)) ...
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bioRxiv - Biochemistry 2021Quote: ... The GST affinity tag was then cleaved using the PreScission Protease (GE Life Sciences) for four hours in cleavage buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: The resin after washing was incubated with Wash Buffer containing PreScission Protease (GE healthcare) to elute RAB11A by cleavage at 4 °C for 16 hrs ...
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bioRxiv - Microbiology 2020Quote: ... A 20 μL sample of Precission protease (2 U/μL; catalogue # 27084301, GE Healthcare) was added and beads were incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... washed and either eluted with glutathione of cleaved with PreScission protease (GE Healthcare, 27084301) overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 then incubated with 1:50 (w/w) PreScission Protease (GE Healthcare) for 18 hrs at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.002% Tween-20) and incubated with Pre-Scission protease (GE Healthcare Life Science) for 18 h ...
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bioRxiv - Biochemistry 2023Quote: ... The GST tag was removed using PreScission protease (GE Healthcare Life Sciences (now Cytiva)) ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were then incubated with GST-tagged PreScission protease (GE Healthcare GE27-0843-01) at a concentration of 50 µL of enzyme per 1 mL of bead slurry ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
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bioRxiv - Biophysics 2019Quote: ... The 12xHis-tag was removed by overnight digestion with 3C protease (GE Healthcare Life Sciences) followed by a purification step with Ni-NTA agarose (QIAGEN ...
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bioRxiv - Biochemistry 2020Quote: ... and protease inhibitors) and passed over an HiTrap Q HP anion exchange column (GE Healthcare). Peak fractions were pooled ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and protein was eluted by ca 40 units PreScission protease (GE Healthcare) for 4 hours at 4°C under rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... and protease inhibitors) was then passed over a glutathione affinity column (Sepharose 4B, GE Healthcare), washed with buffer (40 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion V5-Ydj1 was subjected to site-specific cleavage with PreScission Protease (GE Healthcare) to remove the GST tag ...
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bioRxiv - Cell Biology 2020Quote: ... while Dcp1a was eluted by excision of the GST moiety trough PreScission protease (GE Healthcare) incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was cleaved from the GST tag by incubation with PreScission protease (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The protease was subsequently separated from the eluted proteins using a Glutathione Sepharose (GE Healthcare) packed column ...
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bioRxiv - Biochemistry 2021Quote: ... Excess HRV3C protease was removed by incubation with Ni Sepharose 6Fast Flow beads (GE Healthcare). Fully murine antibodies were produced by cloning the VH regions into a mouse IgG2 expression cassette in the pAbVec background ...
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bioRxiv - Immunology 2023Quote: ... that had encoded an N-terminal His6-tag followed by a PreScission protease (GE Healthcare) cleavage site ...
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bioRxiv - Molecular Biology 2023Quote: ... and bound proteins were eluted by digestion with 20 units of Prescission protease (GE Healthcare) in 0.5 ml of Prescission protease cleavage buffer (50mM Tris-HCl pH 7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were detected with peroxidase-conjugated secondary antibodies (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were detected using horseradish peroxidase-conjugated secondary antibodies (GE Healthcare). To detect the deGRadFP expression from the hb-deGRadFP construct ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega). In addition to the experimental samples ...
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bioRxiv - Pathology 2021Quote: ... horseradish peroxidase linked antibodies were used as a secondary antibody (GE Healthcare). Each signal was detected using Immunostar Zeta or LD (Fuji film ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 mM Imidazole) followed by cleavage with PreScission protease (60 U/ml, GE Healthcare Life Sciences). The beads were applied to a column and the elution was collected and completed to 10 mL with P5 Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and POLGARF bound proteins were eluted by incubation with 1 μl of Prescission Protease (GE Healthcare) in PBS at 4°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... with an N-terminal glutathione transferase (GST) tag followed by a PreScission Protease site (GE Healthcare). The codon optimized cDNA for the ATAD2 BRD was designed by BioBasic and subsequently amplified by PCR and cloned into the pDest15 vector ...
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bioRxiv - Biophysics 2022Quote: The N-terminal 6x His tags were removed from the histones by thrombin protease (GE Healthcare) treatments using 1 unit per 1 mg of protein for 3-5h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... cleaved by PreScission protease and purified by gel filtration chromatography (Superdex 200, 16/60, GE Healthcare) in 20 mM Tris pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... removal of the GST-tagged PreScission protease by immunoaffinity pulldown using Glutathione Sepharose 4B (GE Healthcare) was performed and the purity of GSEC complexes was assessed by SDS-PAGE and Coomassie staining (InstantBlue Protein Stain ...
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bioRxiv - Biochemistry 2021Quote: ... Secondary antibodies (GE Healthcare) were used at 1:5,000 for 1 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... the primary antibody was visualized using 2~5 μg/ml secondary antibody towards specific species of primary antibody labeled with Cy3B (GE Healthcare) or Atto 488 (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (GE Healthcare, NA934, NA931), diluted 1:10,000 ...
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bioRxiv - Plant Biology 2019Quote: ... Cleaved GFP-8His and the His-tagged protease were removed using a HisTrap nickel column (GE Healthcare), and the untagged target AtGAAP3 was harvested from the flow-through ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... cleaved MBP-6X-His and 8X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...