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Citations for GE Life Sciences :
1 - 50 of 1251 citations for Transmembrane Serine Protease 11A TMPRSS11A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Transmembrane stem cell factor (tmSCF) was then isolated using cobalt chelating column (Chelating column; GE Healthcare), and the buffer was exchanged in 1X PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protease cleavage (PreScission: GE Healthcare; TEV-Protease: Biomol) was performed overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... of FZD7 containing the epitope for the F7-Ab (Glycine 168 to Serine 209) was cloned into the bacterial GST expression vector pGEX-4T3 (GE Healthcare Life Sciences). The epitope was shortened from either end through sequential cloning to produce 10 different length epitopes ranging from the original 42 aa to 8 aa ...
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bioRxiv - Cell Biology 2020Quote: ... PreScission Protease (GE Healthcare) was added to remove GST from the proteins ...
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bioRxiv - Biophysics 2020Quote: ... The GST was cleaved with GST-tagged HRV 3C protease (PreScission Protease, GE Healthcare). The GST and protease was removed with glutathione sepharose resin ...
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bioRxiv - Biochemistry 2022Quote: ... thrombin protease (2U/mg GE healthcare) was added ...
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bioRxiv - Cell Biology 2023Quote: ... complete protease inhibitor mix (GE Healthcare), 5 percent glycerol (v/v) ...
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bioRxiv - Molecular Biology 2023Quote: ... ‘PreScission Protease’ (GE Healthcare Life Sciences) was used according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... once loaded GST-PreScission Protease (GE Healthcare) was injected into the column and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... by cleavage with PreScission protease (GE Healthcare) which cleaves between GST and Nuf2 in the GST-Nuf2 fusion protein ...
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bioRxiv - Biochemistry 2023Quote: ... Twenty units of PreScission Protease (GE Healthcare) were added ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Sample was incubated with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Biochemistry 2019Quote: ... containing an N-terminal PreScission protease (GE Healthcare) cleavable His6-tag prior to MCS 1 ...
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bioRxiv - Immunology 2021Quote: ... After addition of 12μl PreScission Protease (GE Healthcare), the suspension was incubated under rotation for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... followed by digestion with PreScission protease (GE Healthcare) and further purification by RESOURCE Q 6 mL column chromatography (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... Protease inhibitors were either from GE Healthcare (cOmplete) or Thermo Scientific (Halt− protease inhibitor cocktail) ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged HRV 3C protease (produced in-house) was used instead of TEV protease and a HiLoad 16/600 S200pg SEC column (GE Healthcare) was used instead of a HiLoad 26/600 S200 SEC column ...
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bioRxiv - Biochemistry 2019Quote: ... and subjected to cleavage with PreScission protease (GE Healthcare) for 5 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and treated overnight with GST-PreScission Protease (GE Healthcare). Eluted proteins were further purified over a Superdex 75 16/600 pd column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... protease inhibitor (G Biosciences) and 10mM immidazole (GE Healthcare). The lysate was cleared via centrifugation and loaded to His Spin Trap spin column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... and subjected to cleavage with PreScission protease (GE Healthcare) for 5 h at 4° C ...
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bioRxiv - Neuroscience 2023Quote: ... by over-night cleavage with Prescission protease (GE healthcare) at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and all columns and proteases were obtained from GE Healthcare. Clarified lysate was passed through a GST Gravitrap column ...
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bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... The σΔ1.1 protein was subjected to thrombin protease (GE Healthcare) treatment in PBS buffer (10 hours of incubation at 4 °C with 10 units of protease per mg of protein) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 2% of Protease Inhibitor Mix (GE Healthcare, Uppsala, Sweden). We purified all samples using a 2-D Clean-Up Kit (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins were then digested with PreScission protease (GE Healthcare) to remove the GST tag and further purified by gel filtration (Superose 6 ...
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bioRxiv - Neuroscience 2021Quote: ... 1x protease inhibitor Cocktail (100x, GE Healthcare, 80-6501-23) and 1mM sodium orthovanadate (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The proteins were then digested with PreScission protease (GE Healthcare) to remove the GST tag and further purified by gel filtration (SuperoseTM 6 prep grade XK 16/70 ...
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bioRxiv - Cell Biology 2022Quote: ... MISTIC was finally cleaved off using thrombin protease (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: GST-tagged fusion protein purification and PreScission Protease (GE Healthcare) cleavage of pGEX- 6P-1-encoded GST-fusion proteins was performed at 4°C during dialysis against HN buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The tags were cleaved off by PreScission protease (GE Healthcare) and the proteins were further purified by sizeexclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 4 h with PreScission protease (GE Healthcare). The TAP-ESP beads were used directly ...
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bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
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bioRxiv - Biophysics 2019Quote: ... PreScission protease was removed by binding to GST-Sepharose (GE Healthcare) for 2 h and buffer exchange with centrifugal filter devices (10,000 MWCO ...
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bioRxiv - Microbiology 2019Quote: ... the GST-tag was cleaved off using PreScission protease (GE Healthcare) in 50 mM Tris/HCl (pH8) ...
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bioRxiv - Biochemistry 2019Quote: ... 0.5 mM PMSF and complete EDTA-free protease inhibitors (GE Healthcare). The S100 fraction of lysate supernatant was passed through PD10 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... PreScission protease and pGEX-6P1 vector were purchased from GE Healthcare. All other chemicals were purchased from VWR Scientific or Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... equipped with a cleavage site (recognised by PreScission Protease; GE Healthcare), was inserted downstream of the leader to facilitate affinity purification ...
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bioRxiv - Plant Biology 2021Quote: ... MBP tagged L7TIR was incubated with by PreScission protease (GE Healthcare) at 4°C or 22°C for 12 hr to remove the MBP tag ...
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bioRxiv - Plant Biology 2021Quote: ... tags could not be completely removed by PreScission protease (GE Healthcare). Arabidopsis Nudt7 (residues 1-322) ...
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removal by PreScission protease (27084301, GE Healthcare) and Glutathione Sepharose 4B affinity subtraction purification ...
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bioRxiv - Cell Biology 2023Quote: ... The GST tag was cleaved off by PreScission protease (GE Healthcare). The GST tag of BAR domains did not inhibit liposome binding or tubulation ...
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bioRxiv - Biophysics 2020Quote: ... Bound proteins were cleaved with Thrombin Protease (GE Healthcare, 27-0846-01) overnight at room temperature in Thrombin Buffer (140 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... and the TEV protease was removed by a Nickel column (GE healthcare). Omicron RBD protein was purchased from Sino Biological ...
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bioRxiv - Microbiology 2020Quote: ... and protease inhibitor) and purified by nickel affinity chromatography (GE healthcare Lifesciences). E ...
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bioRxiv - Cell Biology 2019Quote: ... The HRV3C protease was subsequently removed using a GSTrap 5mL column (GE Healthcare). The sample was further purified and concentrated using Ni-NTA agarose beads ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...