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Citations for GE Life Sciences :
101 - 150 of 1251 citations for Transmembrane Serine Protease 11A TMPRSS11A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The GST part was cleaved off from the recombinant protein using PreScission Protease (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... thrombin or PreScission protease and proteins were further purified by ion exchange chromatography (Source 15Q, GE Healthcare) and gel filtration chromatography (Superdex 200 ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved GST-tag and GST-tagged TEV protease was removed by Glutathione Sepharose 4B (GE Healthcare) and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... the Fc tag was cleaved by O/N incubation with GST-tagged 3C PreScission Protease (GE Healthcare) in cleavage buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... cleaved MBP-6X-His and 6X-His-TEV protease were removed using a HisTRAP column (GE Healthcare).
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bioRxiv - Developmental Biology 2022Quote: ... cleaved on the column with 3C protease followed by size exclusion chromatography (HiLoad Superdex 200, GE Healthcare). The final product was collected in 25 mM HEPES pH 7.8 ...
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bioRxiv - Biophysics 2023Quote: ... and Cdc6 was released from the beads by digestion with Precision protease (GE Healthcare #27-0843-01) at 4 °C for 16 h ...
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bioRxiv - Biophysics 2023Quote: ... VD1 was separated from TEV protease and further purified on a HiTrap SP XL column (GE Healthcare). Flow-through fractions containing VD1 were pooled and concentrated to ∼ 1 mM by centrifugation in Amicon Ultra-15 ultrafiltration devices with a 10 kDa molecular weight cut-off (EMD Millipore ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). MBP-CENP-E2605-2701 was purified using the same lysis buffer without imidazole and an MBP-Trap HP coloumn (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... the protein was cleaved with Tev protease and purified on a HiTrap Q HP column (GE Healthcare) using a salt gradient ...
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bioRxiv - Plant Biology 2021Quote: ... Monoclonal anti-actin (plant) antibody detected by the secondary antibody (GE Healthcare; NA931-1ML) was used as a loading control (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... The secondary antibodies were HRP-linked antibodies against rat or mouse IgG (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... antibodies and the secondary anti-rabbit horseradish peroxidase-linked IgG antibody (NA944V, GE Healthcare for western blotting and 7074S Cell signaling Technology for IHC ...
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bioRxiv - Biochemistry 2021Quote: ... HRP linked antibodies (GE Healthcare), respectively.
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bioRxiv - Biochemistry 2023Quote: ... Anti-GST antibody (GE Healthcare) was used for the detection of GST-tagged bromodomains.
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were isolated from the purified GST fusion proteins by cleaving with PreScission protease (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... cleaved on the column with 3C protease and followed by size exclusion chromatography (HiLoad Superdex 200, GE Healthcare). The final product was collected in 25 mM HEPES pH 7.8 ...
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bioRxiv - Cell Biology 2019Quote: GST tag was removed from GST-Ana2 by incubating the resin-bound protein with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Microbiology 2020Quote: ... cleavage products from Ulp protease cleavage were separated by additional ion-exchange column (GE healthcare, MonoS G5/50) using a KCl gradient of 0.1M to 1M in MonoS-Sucrose buffer (20% sucrose ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies were horseradish peroxidase (HRP)-conjugated anti-mouse antibody (NA931, GE Healthcare Life Sciences) and HRP-conjugated anti-rabbit antibody (NA934V ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were detected with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, IL). Membranes were rinsed in PBS + 0.05% Tween-20 before visualization.
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bioRxiv - Microbiology 2019Quote: ... as primary antibody and an HRP-conjugated anti-mouse as the secondary antibody (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies include ECLTM anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (#NA934V, GE Healthcare) and ECLTM anti-mouse IgG Horseradish Peroxidase linked whole antibody (#NA931V ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody (ECL anti-mouse IgG horseradish peroxidase linked whole antibody) (GE Healthcare) was applied for 1 h in 1% skimmed milk in TBST ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were detected by an HRP-conjugated donkey anti-rabbit secondary antibody (GE Healthcare; # NA934V) incubated in PBST for 1h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Peroxidase-labelled anti-mouse IgG antibody and anti-rabbit IgG antibody (GE Healthcare, Chicago, IL, USA) were used as secondary antibodies for immunoblotting ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were detected with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Il USA). Membranes were rinsed in PBS + 0.05% Tween-20 and visualized by ECL in an ImageQuant LAS 4000mini (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase-conjugated antibodies (anti-mouse/rabbit IgG HRP-linked whole antibody donkey; GE Healthcare) were used ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were detected by an HRP-conjugated donkey anti-rabbit secondary antibody (#NA934V; GE Healthcare) or HRP-conjugated donkey anti-mouse secondary antibody (#NXA931 ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM PMSF and 1x protease-inhibitor cocktail) and loaded onto a Superose™ 6 Increase column (GE Healthcare). Twenty-four fractions of 0.5 mL were collected and precipitated as described (Esteve-Bruna et al. ...
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bioRxiv - Biophysics 2021Quote: ... The TEV-protease cleaved sample was loaded onto a 5 mL Q-Sepharose HP column (GE Healthcare Life Sciences), equilibrated in low-salt buffer and maintained at ∼4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were cleaved overnight with 3C protease followed by gel filtration on Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-tag and Thrombin protease were removed via heparin affinity chromatography (HiTrap Heparin 5 ml FF, GE Healthcare). Cas12a was eluted via an elution gradient with 1 M NaCl (20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... In-gel fluorescence of the protease-protected proOmpA-FM was recorded and quantified (ImageQuant TL, Cytiva/GE Life Sciences). The background signal was subtracted using the implemented Local average algorithm ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-linked antibody (NA934; GE Healthcare) as secondary antibody.
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bioRxiv - Cell Biology 2019Quote: ... HRP-linked whole antibody (GE Healthcare) and polyclonal rabbit anti-goat (Dako) ...
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bioRxiv - Molecular Biology 2019Quote: ... HRP conjugated second antibodies (GE Healthcare) were used at a concentration of 1:5000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution and anti-rabbit secondary antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary antibodies (GE Healthcare) were diluted 1:10,000 in TBS + 0.1% Tween-20 and applied to the membrane for 1 hr ...
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bioRxiv - Microbiology 2020Quote: ... IgG1 or IgG2a antibodies (GE Healthcare). The plates were washed and developed with 50 μl of TMB substrate per well until blue color appeared ...
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bioRxiv - Cell Biology 2019Quote: ... Species-appropriate secondary antibodies (GE Healthcare) and ECL Prime Western Blotting Detection reagent (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... Secondary antibody (GE Healthcare, Buckinghamshire, UK) was used in a 1:2000 titer in 5% milk/Tris-buffered saline–Tween 20 for 1 hour at room temperature with agitation ...
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bioRxiv - Synthetic Biology 2022Quote: ... or anti-rabbit antibody (GE Healthcare) as secondary antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... HRP-conjugated secondary antibodies (GE Healthcare) were used ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated anti-rabbit IgG donkey antibody and HRP-conjugated anti-goat IgG donkey antibody (GE Healthcare), HRP-conjugated Protein-G (abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-CSP repeat antibody – mAB 3D11 (Yoshida et al., 1980) and HRP bound antibodies (GE healthcare, Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare). Protein was further purified using Q and Superdex G75 columns (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Microbiology 2022Quote: ... The TEV protease treated suspension was then transferred into the new columns containing 500 μl Calmodulin Sepharose 4B (GE Healthcare) and 6 ml of CBB buffer [150 mM NaCl ...