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Citations for GE Life Sciences :
2301 - 2341 of 2341 citations for 3 Chloro 5 fluoro 4' morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The diluted peak fractions were loaded at 2 mL/min in Buffer A onto a 5 mL HiTrap Heparin column (GE Healthcare Life Sciences) and eluted at 2 mL/min with a 50 mL gradient from 30% to 100% Buffer B ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was filtered (0.45 μm filter) and loaded on a 5 mL HisTrap Fast Flow column (GE Healthcare, 17-5255-01). The purification was conducted on the bench using a peristaltic pump ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were washed for 5 min three times with TBS-T before incubating at room temperature for 1 h with the secondary antibody (anti-rabbit HRP conjugated anti-rabbit IgG [GE Healthcare] diluted 1:5,000 in 3% BSA/ TBS-T). After washing the membranes three times in TBS-T buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
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bioRxiv - Immunology 2020Quote: HEK293T cells were grown at 37°C to approximately 80% confluence in 10 cm tissue culture dishes using DMEM with 5% Hyclone Fetal Clone II (GE Healthcare Lifesciences, Logan, UT) and 1X antibiotic and antimycotic (Gibco ...
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bioRxiv - Biochemistry 2021Quote: Nickel Immobilised Metal Ion Affinity chromatography (Ni2+-IMAC) for protein purification was conducted using a HisTrap® HP 5 mL column (GE Healthcare, Munich, Germany) coupled to a BioLogic DuoFlow™ Chromatography System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... The culture medium containing the secreted ACE2 was subsequently loaded onto an ion-exchange column (HiTrap 5 ml Q FF anion exchange chromatography column; GE Healthcare, U. S. A.), and subsequently eluted by a linear salt gradient ranging between 10 and 1000 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... were purified by immobilized metal affinity chromatography (IMAC) using a HisTrap HP 5 mL column mounted on an Äkta Purifier System (GE Healthcare, Chicago, IL, USA). Fractions containing the His6-Sumo-peptide fusion in elution buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...
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bioRxiv - Genomics 2020Quote: ... The mixture was left to react for 30 minutes protected from ambient light and the crude reaction mixture was passed through a NAP-5 column (GE Healthcare Life Sciences, 17085301) for collection and purification of the fluorophore-conjugated secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... Filtrates were then filtered gently by vacuum (<400 mbar under-pressure) over pre-combusted GF/A filters (> 5 h at 460°C; 47 mm, GE Healthcare Whatman, Buckinghamshire, UK), and finally over 0.22 µm pre-cleaned44 hydrophilic PVDF filters (<600 mbar under-pressure ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified in two steps by immobilized metal chelate affinity chromatography (IMAC) using 5 mL HisTrap FF columns (GE Healthcare, Chicago, Illinois, USA) as previously described ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was dried for 30 min at 80° C in a drying oven before being blocked with 5% ECL advanced blocking reagent (GE Healthcare, Piscataway, NJ, USA) for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... We separated equal amounts of protein on 5-20% SDS‒PAGE gels (Wako, Osaka, Japan) and transferred them to Hybond-P membranes (GE Healthcare, Piscataway, NJ, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was further purified via immobilized metal affinity chromatography purification using a 5 mL HisTrap FF Crude column (GE Healthcare, Piscataway, NJ, U.S.A.). Once bound ...
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bioRxiv - Plant Biology 2024Quote: ... The fusion protein His-SUMO-ApcI was purified by loading the cell lysate supernatant to a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... the protease was diluted to 10 mL with TRIS pH 7.2 and NaCl (final conditions of 20 mM Tris-HCl pH 7.2 + 500 mM NaCl (Buffer A)) in preparation for purification using a 5 mL HisTRAP Benzamidine column (GE Healthcare, Cat. #17-5144-01). The column was equilibrated using the AKTA Pure L1 FPLC (GE Healthcare) ...
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Stepwise Isolation of Diverse Metabolic Cell Populations Using Sorting by Interfacial Tension (SIFT)bioRxiv - Bioengineering 2024Quote: ... Cells were grown at 37 °C in a 5% CO2 atmosphere supplemented with 10% fetal bovine serum (HyClone, GE Healthcare Life Sciences, Logan, UT) and 2% v/v penicillin-streptomycin (10 000 units/mL-10 000 μg/ mL ...
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bioRxiv - Immunology 2024Quote: ... The obtained cell suspension was added to 4.5 ml RPMI medium and slowly layered on 5 ml Ficoll® (17-1440-02, GE Healthcare, Sigma-Aldrich®) in a 15 ml Falcon tube ...
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bioRxiv - Biochemistry 2024Quote: ... Two volumes of 50 mM Tris/HCl pH 8.0 and 2 mM DTT were added to the pooled fractions before loading on a 5 ml HiTrap™ Q-Sepharose High-Performance column (GE Healthcare, Munich, Germany) column equilibrated with 25 mM Tris/HCl pH 7.6 and 2 mM DTT ...
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bioRxiv - Biochemistry 2024Quote: ... Two volumes of 50 mM Tris/HCl pH 8.0 and 2 mM DTT were added to the pooled fractions before loading on a 5 ml HiTrap™ Q-Sepharose High-Performance column (GE Healthcare, Munich, Germany), equilibrated with 25 mM Tris/HCl pH 7.6 and 2mM DTT ...
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bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude). The column was equilibrated in 500 mM sodium chloride ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants were filtrated and subjected to affinity chromatography using a 5 mL HisTrap HP His-tag purification column (GE healthcare, cat. # GE29-0510-21) operated by a peristaltic pump (Cytiva ...
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bioRxiv - Biochemistry 2021Quote: ... DrrA-OCRL or DrrA-INPP5E were then loaded onto a 1 mL MonoS (5/50 GL) cation exchange column (GE Healthcare, Cat# 17-5168-01) equilibrated in 20 mM HEPES pH 7 ...
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bioRxiv - Microbiology 2020Quote: ... Enrichments were performed in test tubes containing 5 mL of M63 minimal media and a 1 x 10 cm strip of Whatman #1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA) pressed against the side of the tube (30) ...
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bioRxiv - Biochemistry 2022Quote: Verification of the monomeric or dimeric state of those constructs was obtained by SEC-MALS experiments with the injection of 5 mg/mL of each protein into a Superdex 200 10/30 Increase column (GE Life Sciences, Velizy-Villacoublay, France) mounted on a HPLC Ultimate 3000 (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Immunology 2023Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Clarified lysate was loaded onto a nickel (II) 5 mL HisTrap packed with Sepharose® 6 fast flow agarose beads (GE Healthcare Biosciences, NSW, Australia) using an ÄKTApure™ system (GE Healthcare Biosciences) ...
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bioRxiv - Plant Biology 2024Quote: ... at a 1 to 20 ratio in Buffer A overnight at 4°C followed by a subtractive His trap purification using a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK) (GE Healthcare, Little Chalfont, UK) passing the mixture of His-SUMO-ApcI with ULP through the HisTrap column retaining His-SUMO as well as ULP and collecting the flowthrough containing tagless ApcI ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was then filtered through a 0.2 µm syringe filter before being loaded onto 2 x 5 mL StrepTrap HP Columns (GE Healthcare Life Sciences 28-9075-47), equilibrated in Step A Buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Pathology 2023Quote: ... homogenates were centrifuged at 500 x g for 5 min at RT and cell pellets were resuspended in a 36% Percoll solution (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) containing 100 UI/mL of heparin (HIBOR 5000 UI ...
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bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...