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Citations for GE Life Sciences :
1801 - 1850 of 2341 citations for 3 Chloro 5 fluoro 4' morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... peak fractions were diluted to 100 mM NaCl and loaded onto a 5 ml HiTrap Q XL column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.3 ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant containing the protein was loaded on a 5 mL HiTrap Ni-chelating column (GE Healthcare, Piscataway, NJ), washed ...
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bioRxiv - Biophysics 2021Quote: ... Pooled eluate fractions were adjusted to 150 mM NaCl and purified on a 5 ml HiTrap Heparin column (GE Healthcare) using a linear gradient from 150 mM NaCl to 1 M NaCl in buffer A ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μL of Amersham ECL Rainbow Marker was loaded as a molecular weight standard (GE Life Sciences, Pittsburgh, PA). Proteins were transferred onto nitrocellulose and incubated with primary antibody ...
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bioRxiv - Biophysics 2021Quote: ... nickel affinity chromatography was performed where the lysate was loaded onto a 5 mL HisTrap™ Excel column (GE Healthcare), followed by a wash with 20 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: Single cysteine mutants of ColE9 or the ColE9 DNase domain were desalted on a 5 ml HiTrap column (GE Healthcare) equilibrated with binding buffer (20 mM potassium phosphate pH 7 ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble cell lysate obtained after centrifugation was loaded onto a 5 ml HisTrap FF HP nickel column (GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The co-expressed complex of ctCyclin H and ctMAT1short was loaded on a 5 ml StrepTrap HP column (GE Healthcare) equilibrated in lysis buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was centrifuged at 38,000 ×g and the supernatant was loaded onto a 5 mL HisTrap Ni-NTA column (GE Healthcare). The column was washed with HisTrap Buffer and protein eluted with a linear gradient of imidazole from 25 to 300 mM in HisTrap Buffer over 10 column volumes.
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bioRxiv - Systems Biology 2021Quote: ... The supernatants obtained after this step were loaded in 5-mL His-trap columns (GE Healthcare Systems, Chicago, IL, USA) pre-equilibrated with buffer A supplemented with 100 mM NaCl and 20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was filtrated through a O.22μm pore filter and loaded on a 5 ml pre-packed Hitrap Q FF column (GE Healthcare) equilibrated with buffer A (50mM bis-tris-HCI pH 6.0 ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was subjected to immobilized-metal affinity chromatography (IMAC) using a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB1 containing 20 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation for 30 min at 47,000 xg and the supernatant was loaded onto a 5 ml GSTrap column (GE Healthcare). The resin was washed by the lysis buffer and bound protein was eluted with elution buffer of 100 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Human IgGs were isolated from heat-inactivated plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) as described (24).
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bioRxiv - Cell Biology 2021Quote: ... The lysate was cleared by centrifugation and it was applied to a 5 ml GSTrap FF column (GE Healthcare, 17513101) to purify GST-proteins ...
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bioRxiv - Cell Biology 2021Quote: ... His-Bro1ΔAPRR purified from BL21-DE3 using Ni2+-affinity chromatography (5 ml HiTrap Chelating FF column; GE Healthcare, United Kingdom). GST-Bro1V(370-709 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation for 1 hour at 38,700 x g and passed over a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 15 column volumes of 20 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM TCEP) and the sample was loaded onto a 5 mL HiTrap Heparin HP affinity column (GE healthcare) pre-equilibrated with 5% buffer B (50 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was consecutively filtered through 0.45 µm and 0.22 µm PES syringe filters prior to loading onto a 5 ml HisTrap nickel affinity column (GE Healthcare) preequilibrated with Buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
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bioRxiv - Biochemistry 2022Quote: ... The eluate of HIC-EBA was subjected to affinity chromatography using Strep-Tactin® column (5 ml cartridge, GE Healthcare Life Science ...
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bioRxiv - Biochemistry 2022Quote: ... to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare). A linear 0-75 mM histidine gradient with elution buffer was used to elute proMbxA ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant of the lysate was filtered through a 0.22 µm membrane filter and loaded onto a Ni-NTA column (HiTrap, 5 ml, GE Healthcare) using a low imidazole buffer (Buffer C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered using a 0.2 μl filter and flown over a 5 ml NiNTA IMAC column (GE Healthcare) equilibrated with buffer A (50 mM KH2PO4 pH 7 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered using a 0.2 μl filter and flown over a 5 ml StrepTactin-Sepharose column (GE Healthcare) equilibrated with buffer A (50 mM MES-NaOH ...
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bioRxiv - Microbiology 2022Quote: ... and the supernatant was filtered using a 0.2 μl filter and flown over a 5 ml NiNTA Immobilised Metal Affinity Chromatography (IMAC) column (GE Healthcare) equilibrated with buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged and the supernatant was filtered through a 0.45 μm filter and subjected to a His-tag affinity chromatography using a 5 mL HisTrapFF (GE Healthcare) and the ÄKTAprime plus system (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was supplemented with 50 mM imidazole and loaded onto a 5 mL His-Trap FF column (GE Healthcare), washed with 6 column volumes (CV ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was filtered using 0.2 μm filters and buffer exchanged in storage buffer (20 mM Tris-HCl pH7.4, 200 mM NaCl and 5% glycerol) using PD-10 desalting columns (GE healthcare). The desalted sample then was aliquoted and stored at -80°C until the one-step purification/ immobilisation.
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bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 15,000xg for 5 min and supernatants were incubated with 50 μL of Streptavidin Sepharose High Performance beads (GE Healthcare) for 1 h rotating ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mL imidazol gradient) or by size exclusion chromatography (SEC) using a Superdex® 75 10/300 GL (GE Healthcare) with a flow rate of 0.5 mL/min and dialysis buffer as running buffer ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was filtered using a 0.2 μM filter and loaded onto a 5 ml HisTrap HP Ni-affinity column (GE Healthcare). Protein was eluted in a gradient of 10 to 300 mM imidazole over five column volumes ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were pelleted and lysed by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 500 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 mL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing MBP-AIM2 were pooled and purified by ion-exchange chromatography using 5 mL HiTrap-SP column (GE Healthcare) equilibrated in 20 mM HEPES ...
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OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scalebioRxiv - Systems Biology 2022Quote: ... IgG was isolated from 5 μl of serum using 30 μl of Protein A Sepharose (GE Healthcare, Eindhoven, The Netherlands). Sample mixtures were incubated under agitation at 650 rpm for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... All immunoblots were visualized with ECL Plus Detection Reagent and scanned with a Typhoon 5 Variable Mode Imager (GE Healthcare). Results are expressed as means ± SEM and expressed relative to the “no ligand” condition.
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bioRxiv - Neuroscience 2022Quote: ... All immunoblots were visualized with ECL Plus Detection Reagent and scanned with a Typhoon 5 Variable Mode Imager (GE Healthcare). Results are means ± SEM from 5-6 mice per genotype and expressed relative to TrkAWT mice.
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bioRxiv - Immunology 2022Quote: ... The lysed cells were centrifuged and only the supernatant with the soluble proteins was then further filtered with 0.45 µm filters and loaded onto two connecting 5 mL HisTrap IMAC FF columns (GE Healthcare) which were pre-equilibrated with 50mM phosphate buffer with 500 mM NaCl and 20 mM imidazole at pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... to remove cell debris the supernatant was filtered through a 0.45-μm pore size filter and then loaded onto a 5-ml Ni-HiTrap column (GE Healthcare) that had been pre-equilibrated in the same lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plates were incubated at 37° C and imaged after 20 h with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare) using the following settings ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation for 45 min at 48.000 xg and loaded onto a HisTrap FF 5-ml column (GE Healthcare). Eluted proteins were incubated with TEV protease at 4 °C overnight and both the His6-tag ...
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bioRxiv - Plant Biology 2020Quote: ... 20 mM imidazole pH 7.5 was added and the solubilized membranes were loaded on a pre-equilibrated 5 ml Ni-NTA column (GE Healthcare) at 3 ml/minute ...
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bioRxiv - Microbiology 2021Quote: ... and were eluted with CA loading buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM BME) using AKTAprime plus (GE Healthcare). CA monomer fractions were concentrated using Amicon Ultra-10K device (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... The eluate was then diluted 3x with 20 mM Tris-HCl pH7.5 and passed over a 5 mL Q-sepharose column (GE Healthcare). The Flow through containing the MIF protein was then again loaded on a 5 mL CM sepharose column ...