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Citations for GE Life Sciences :
2151 - 2200 of 2341 citations for 3 Chloro 5 fluoro 4' morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The proteases were then loaded onto an equilibrated MonoS 5/50 GL ion exchange column (GE Healthcare Cat. #17-5168-01) and washed with 10 column volumes (CV ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was cleared by centrifugation and loaded onto two serially connected HisTrap 5 ml columns (Cytiva) which were connected to a ÄKTA FPLC system (GE Healthcare). Columns were washed with lysis buffer and bound proteins were eluted with a linear imidazole gradient (20 – 500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... DsbA-RbmB was purified from the cleared lysate by loading the supernatant onto a cobalt-charged 5 mL His-trap HF (GE Healthcare) equilibrated with high-salt TBS buffer (20 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 mM DTT and loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted with a 1.0 to 0.4 M linear gradient of (NH4)2SO4 for 8 CV at a flow rate of 1 ml.min-1 ...
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bioRxiv - Biochemistry 2024Quote: ... Dried gels were then exposed to a storage phosphor screen for six days before acquiring radiograms using a Typhoon 5 Imager (GE Healthcare). All experiments were performed in three independent replicates that were carried out on three separate days ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was filtered through a 0.45 µm membrane and loaded onto a nickel affinity column (5 mL GE Healthcare HisTrap). The protein was eluted using a gradient from 20 to 500 mM imidazole (solution B ...
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bioRxiv - Biophysics 2024Quote: ... The eluate was concentrated with 5 kDa spin concentrator and loaded on a Superdex 200 Increase 10/300 column (GE Healthcare) using 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was separated from MBP using cation-exchange chromatography with a 5 ml SP Sepharose HiTrap column (GE Life Sciences). Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15 ...
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bioRxiv - Cell Biology 2024Quote: ... Clarified lysates were loaded using a sample pump or super-loop (Cytiva) on a 5 mL Strep-Trap HP column (GE Healthcare) which was pre-equilibrated with 5 column volumes (CV ...
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bioRxiv - Cell Biology 2024Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 centrifugal concentrators (10 kDa and 5 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blots were visualized and quantified with enhanced chemiluminescence and imaging on a Fuji LAS 4 000 image station (GE Healthcare, Chicago, IL, US)4.
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Genomics 2021Quote: ... #S0282).[1] Reverse transcription was performed in 1.5 mL microfuge tubes under end-over-end rotation using a modified Reverse Transcription mix (1x Maxima H-RT buffer, 4% Ficoll PM-400 (GE Healthcare, #17-0300-05), 3 mM MgCl2 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Bioengineering 2020Quote: ... depending on machine availability and the type of imaging performed: LOGIQ Book XP with 8L-RS 4-12 MHz linear array probe (GE Healthcare, Milwaukee, WI, USA), Aixplorer with L14-5 probe (Supersonic Imagine ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
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bioRxiv - Immunology 2024Quote: ... to 2 ml and subsequently loaded on a Sepharose 4 Fast Flow resin column (10 mm x 300 mm; GE Healthcare, Little Chalfont, UK), connected to the ÄKTA pure system (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... a cell suspension containing 20,000 cells per 50uL was prepared in Biolog IF-M1 containing 0.3mM glutamine and 5% dialyzed FBS (dFBS) (Hyclone GE Life Sciences, SH30079.01) and plated into PM-M1 and PM-M2 96-well plates at 50 μL and 20,000 cells per well ...
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bioRxiv - Neuroscience 2021Quote: ... Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Neuroscience 2020Quote: ... Lipofectamine 2000 and siRNAs were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was cleaved with Precission™ protease for 2 hours at room temperature and applied to a 5 mL HiTrap Q HP column (GE Healthcare). Protein was eluted from the Q HP column with a 2M NaCl buffer in a 100 mL gradient ...
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bioRxiv - Cell Biology 2021Quote: ... Figure 5F and Figure 5-figure supplement 1D were acquired and processed by iterative deconvolution using Soft-WoRx software (Applied Precision Ltd.). For Figure 1E ...
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bioRxiv - Bioengineering 2021Quote: ... The dialyzed eluate was further passed through an equilibrated 5 mL heparin column (HiTrap™ Heparin HP) on a FPLC (AKTA pure, GE healthcare) and eluted using a linear NaCl gradient generated from heparin buffers A and B (40 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare). Fractions containing protein of interest were pooled ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... Purification was performed by combing a HisTrapFF column and analytical size-exclusion chromatographic Superdex™ 75 5/150 with an ÄKTAexplorer 100 (GE Healthcare). The human cDNA sequence for the HIF-1α-PAS-B domain was synthesized and subcloned into the prokaryotic expression vector pGEX-4T-1 (Amersham) ...
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bioRxiv - Biochemistry 2020Quote: ... Both the LaGH13_20 and LaGH13_31B enzymes were purified by immobilized metal ion affinity chromatography on 5 ml HisTrap HP columns (GE Healthcare, Uppsala, Sweden) equilibrated with binding buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... diluted two-fold against fresh buffer B and then manually applied at room temperature to a 5 mL HiTrap Heparin cation exchange column (GE Healthcare # 17040701) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... The protein purification from precipitated cell-culture media was done on HisTrap HP 5 ml and HiLoad 16/600 Superdex 75 (GE Healthcare, USA) columns by the method described previously (Zahradník et al. ...
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bioRxiv - Biophysics 2021Quote: ... Bacterial cells were disrupted by sonication and the soluble fraction was loaded on a 5 mL HiTrap Chelating HP column (GE Healthcare - USA) previously equilibrated with same buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The modified Cy3 labeled fragment was generated by labeling an amine-modified RNA containing a 5′ monophosphate ordered from Horizon Discovery with monoreactive Cy3 (GE Life Sciences) and the labeled RNA was purified by reverse-phase HPLC ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 % (v/v) glycerol by FPLC (ÄKTA explorer, Amersham Pharmacia Biotech) using a Superdex™ 200pg 16/60 FPLC column (GE Healthcare). Final protein fractions were analyzed by SDS-PAGE electrophoresis.
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biophysics 2022Quote: ... coli upec-117 CapW at 5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... filtered through a 0.22 μm membrane filter and loaded onto a 5 ml HiTrap Heparin HP column (GE Healthcare, Chicago, Illinois, USA). The column was washed with 10 volumes of Buffer H (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... 10% glycerol at pH 8.2) and loaded onto a 5 mL prepacked Q-sepharose ion exchange column (GE Healthcare Life Sciences, MA) at 1 mL/min ...
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bioRxiv - Microbiology 2020Quote: ... Crude extract was then prepared and subjected to purification by nickel-nitrilotriacetic acid (Ni2+−NTA) metal affinity chromatography using a 5 ml HisTrap™ HP column (29-0510-21; GE Healthcare). MenI was eluted at 200 mM imidazole ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
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bioRxiv - Bioengineering 2021Quote: Several DSAs were performed to guide the intervention by injecting approximately 5-10 ml of a half-strength contrast agent (CA) (50% of Omnipaque 240 (GE Healthcare, USA) and 50% of a saline solution ...
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bioRxiv - Microbiology 2021Quote: ... Fusion events were quantified by detecting GFP+ pixels after 18 hours incubation at 37°C 5% CO2 using Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using the ImageQuant TL 8.2 image analysis software (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by vacuum filtration onto glass microfiber filters (grade GF/C, 125 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 50 mL centrifuge tubes (Sarstedt ...
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bioRxiv - Genomics 2020Quote: ... mRNA was extracted from approximately 5 μg of the total RNA samples using Sera-Mag oligo-dT beads (GE Healthcare Life Sciences). The mRNA was fragmented and used to prepare sequencing libraries according to the Illumina TruSeq Stranded mRNA sample preparation kit ...