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Citations for GE Life Sciences :
1901 - 1950 of 2341 citations for 3 Chloro 5 fluoro 4' morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA eluate was applied to size exclusion chromatography on a Superose 6 Increase 5/150 column (GE Healthcare) in basic buffer supplemented with 0.003 % LMNG and 0.0003 % CHS.
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) and further purified via size exclusion chromatography (SEC) in an S75 5/150 GL increase column (GE Healthcare). Protein collected from SEC was normalized to 1 μM where possible.
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples were subjected to electrophoresis through 5% native polyacrylamide gel in 0.5x TBE buffer and imaged with 9410 Typhoon variable mode imager (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... the KhpA and KhpB-ΔN were secondly purified by cation-exchange chromatography (HiTrap SP 5 ml, GE Healthcare Life Science) followed by SDS-PAGE ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The solubilized protein suspension was ultracentrifuged at 100,000 x g for 1 hour before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with a purification buffer supplemented with 20 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: The Ni-NTA elution was immediately loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare, Chicago, IL, USA) that was equilibrated with 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... MukBEF was largely found in the flowthrough and was applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 CV of 10 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was collected and applied to a 5 mL pre-equilibrated HiTrap Phenyl HP hydrophobic interaction column (GE Healthcare) using a running buffer with 20 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... 5 mL of the samples were applied to a size exclusion chromatography column (Superdex 200pg, HiLoad 16/60, GE healthcare) equilibrated in SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was filtered through an 0.8 µm membrane and loaded onto a 5 mL HisTrap HP column (GE Healthcare), pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... and the sample was loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted at 1 M (NH4)2SO4 at a flow rate of 1 ml.min-1 ...
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bioRxiv - Biophysics 2024Quote: ... RbmB was purified from the cleared lysate by loading the supernatant onto a 5 mL His-trap HF (GE Healthcare) equilibrated with high-salt TBS buffer (20 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... The resultant supernatant was filtered through a 0.8 μm membrane before being loaded onto 5 mL HisTrap FF nickel column (GE Healthcare) pre-equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2024Quote: ... The eluate was then exchanged into 20 mM Tris HCl pH 8.0 by dialysis and loaded onto a 5 mL Q-HP column (GE Healthcare). The different forms were isolated by using 50 column volumes (250 mL ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble material was subsequently filtered using a 0.22 mm pore size filter and was loaded onto a 5 ml HisTrap HP column (GE Healthcare), which was pre-equilibrated with lysis buffer and immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified by salt gradient ion exchange chromatography using a Mono Q 5/50 GL column (GE healthcare) and Gradient buffers (20 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were centrifuged and filtered prior to successive FPLC purification on HisTrap HP (5 mL, GE Healthcare, 17-1154-01), HiTrap Q HP (5 mL ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were centrifuged at 13,000 RPM for 5 minutes at 4°C and the supernatant was transferred to a new tube and cleared of residual IgG by rotation with Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The affinity tag for Munc18 was cleaved by 2 mg of TEV in PBS with 400 mM imidazole overnight at 4℃ and additional purification was performed using superdex 200 10/300 GL (GE Healthcare, Piscataway, NJ) gel filtration column in TBS buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred at 4 °C for 16 hours to a nitrocellulose membrane (Ø 0.2 µm GE Healthcare, Whatman, 10401396) through a wet transfer system (transfer buffer ...
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag with the 3C protease (MERCK) ...
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were exposed to a phosphor screen for a minimum of 4 hours and scanned using a Storm 820 phosphorimager (GE Healthcare Life Sciences). The bound fraction of each DNA substrate was plotted as a function of PolY concentration and fit to Equation 2 to obtain the dissociation constant ...
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bioRxiv - Biochemistry 2020Quote: ... Resolved products were exposed to a phosphor screen for a minimum of 4 hours and scanned with a Storm 820 phosphorimager (GE Healthcare Life Sciences). The product signals were quantified using ImageQuant software (v5.0 ...
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bioRxiv - Biochemistry 2021Quote: ... or by sonication and cleared lysate was obtained by centrifugation (24,446 x g; 30 min; 4°C) and filtration using a Whatman™ folded filter (GE Healthcare, now Cytiva).
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bioRxiv - Molecular Biology 2020Quote: ... Sample was washed twice with HBSS and then centrifuged for 15 minutes at 1800 RPM at 4° C through 25% Percoll (GE HealthCare, Pittsburg, PA). The resulting fatty upper and fluid middle layers were removed from the pellet ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentrated supernatant was loaded on a Tricorn 10/300 Sepharose 4 Fast Flow (S4FF) size exclusion chromatography column (GE Healthcare, Buckinghamshire, UK) connected to the ÄKTA prime system (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Bacterial lysates were cleared by centrifugation at 10,000 g for 20 min at 4°C and applied to a HisTrap HP column using an AKTA Prime protein purification system (GE Healthcare, Life Sciences). Bound protein was eluted with a linear imidazole gradient ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Microbiology 2022Quote: 5 μg of input and 10 μl of output was resolved by SDS-PAGE by 4-20% acrylamide gels and analyzed by in-gel fluorescence using a Typhoon FLA 7000 (GE Healthcare Life Science) using the rhodamine channel to detect TAMRA-labeled proteins.
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were centrifuged for 20 min at 23000 × g at 4°C and filtered using Whatmann Mini-Uniprep filtering vials (GE Healthcare, Buckinghamshire, UK) with a regenerated cellulose membrane (0.2-μm pore size).
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bioRxiv - Immunology 2022Quote: ... and the resultant lysate was incubated overnight at 4 °C with 1.5 ml of glutathione-Sepharose 4B bead slurry (GE Healthcare, 17-0756-01). Beads were washed three times in wash buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... bacterial extracts were centrifugated for 20 min at 18,000 x g at 4°C and the supernatant was incubated with 1.5 ml of a GSH-sepharose 4B resin (GE Healthcare 17-0756-01) for 1.5 h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated at 4°C for at least 1 hr with ∼40 µL of appropriate bead slurry (nProtein A Sepharose, GE Healthcare, 5280-01). Following incubation ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was separated by ultracentrifugation at 100 000 x g for 30 min at 4 °C and supernatant was loaded onto a HisTrap HP column (GE Healthcare, Freiburg, Germany) preequilibrated with 50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies (anti-UBAP2L or anti-PLK1) as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...