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Citations for GE Life Sciences :
2101 - 2150 of 4633 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... and proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 NC, GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected by enhanced chemiluminescence (ECL Prime Western blotting detection reagent, GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated on a HiLoad Superdex 200 Increase 10/300 column (GE Healthcare) and plotted against BioRad Standards to determine size ...
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bioRxiv - Plant Biology 2021Quote: The purified proteins were loaded onto a Superdex 200 GL 10/300 (GE Healthcare) column and eluted with a buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Developmental Biology 2020Quote: ... and proteins were detected using Amersham Western Blotting Detection Reagent (GE Healthcare, Chicago, IL). Western blot membranes were probed with mouse anti-RFP (RF5R Invitrogen ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Protein was detected using an Amersham ECL Western Blotting Analysis System (GE Healthcare RPN2109), and developed onto film or imaged using a Biorad Chemidoc gel imager ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Nickel NTA columns (17-5248-01, GE Healthcare, USA) or beads (7066-4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the target proteins were loaded onto a HiTrap Q column (GE Healthcare, USA). An increasing linear gradient of NaCl concentration was applied onto the HiTrap Q column for further purification ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein levels were quantified with the ImageQuant TL software (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2020Quote: ... protein was immobilized to the CM5 sensor chip (GE Healthcare, Cat#. BR-1005-30) at 6.0 μg/mL concentration using 10mM sodium acetate (pH 5.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... cleaved protein was loaded onto a Superdex 75 10/300 GL column (GE Healthcare) in Ni-buffer A ...
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bioRxiv - Bioengineering 2022Quote: ... and IgG was purified with Protein A sepharose beads (GE Healthcare 17-5280-04).
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bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Genomics 2022Quote: ... Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare), washed and purified according to standard ChIP procedures ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was bound to an anion exchange column (MonoQ 16/10, GE Healthcare) and eluted with a linear salt gradient up to 1 M NaCl ...
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bioRxiv - Cancer Biology 2019Quote: ... Cleared lysates were incubated with protein G Mag Sepharose™ Xtra beads (GE Healthcare) and 1µg of antibody anti-ISG15 per sample for 4h ...
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bioRxiv - Synthetic Biology 2019Quote: Western blotting was carried out following protein transfer onto a nitrocellulose membrane (GE Healthcare) on a TE 77 PWR Semi-Dry Transfer Unit (45 mA/gel and 30 min with a NOVA Blot kit ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was loaded onto a pre-equilibrated QHP ion exchange column (GE Healthcare) and eluted with buffer B containing increasing concentrations of NaCl (0.1-1 M) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were purified by immobilised metal affinity chromatography using nickel-Sepharose resin (GE Healthcare) equilibrated in buffer A ...
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bioRxiv - Plant Biology 2019Quote: ... purified proteins were run down a Superdex™ 75 10/300 column (GE Healthcare) at 0.5 ml/min either alone or mixed to assess complex formation (mixtures were incubated on ice for 2 hours prior to experiment) ...
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bioRxiv - Molecular Biology 2019Quote: ... The immune complexes were then precipitated by Protein G Magnetic Sepharose beads (GE Healthcare) for 4 hours rotating at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... MBP-fusion proteins were purified with amylose resin following the manufacturer’s instructions (GE Healthcare, Pierce ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was passed over a size exclusion column (Superdex 200, GE Life Sciences) in buffer GF (buffer A plus 300 mM NaCl and 1 mM dithiothreitol (DTT)) ...
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bioRxiv - Biochemistry 2020Quote: ... The target protein was further purified using MonoQ and Superdex G75 columns (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... The concentrated proteins were buffer exchanged using a PD-10 desalting column (GE Healthcare) equilibrated with Buffer C (50 mM CAPS ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were purified using the Sepharose beads affinity method (Glutathione Sepharose 4B; GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... Target proteins were further purified on a Superdex-200 16/60 column (GE Healthcare) pre-equilibrated with a running buffer containing 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... the extracts were incubated with 15 μl of protein A sepharose beads (GE Healthcare) on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were routinely desalted using 5 mL HiTrap™ desalting columns (GE Healthcare, USA) using desalting buffer (50 mM Tris-HCl pH7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced by adding (isopropyl β-D-1-thiogalactopyranoside, IPTG; GE Healthcare) to a final concentration of 0.3 mM ...
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bioRxiv - Molecular Biology 2019Quote: ... chromatin lysates were incubated for 4 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Proteins were concentrated and further purified using gel filtration (S75 16/60, GE Healthcare) and ion-exchange (Hi-Load Q column ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then passed over a 5 mL HisTrap HP column (GE Healthcare), washed with 10 CV of Nickel Wash Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was further purified by heparin affinity chromatography (HiTrap Heparin HP, GE Healthcare), eluting with a linear gradient of increasing salt from 150 mM - 1 M NaCl ...
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bioRxiv - Microbiology 2019Quote: ... proteins were transferred onto a nitrocellulose membrane (Amersham Protran 0.45 µM NC (GE Healthcare)) for one hour at 400mA ...
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bioRxiv - Immunology 2020Quote: ... and then purified using the ÄKTA start protein purification system (GE Life Sciences # 29022094). The supernatant was diluted 1:1 with 20 mM sodium phosphate pH 7.4 and then purified using HisTrap Excel columns (GE Life Sciences # 17-3712-05 ...
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bioRxiv - Microbiology 2019Quote: ... Protein samples were applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min pre-equilibrated with dH2O and buffer I (25 mM HEPES/NaOH ...
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bioRxiv - Microbiology 2019Quote: ... Protein sample was applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min pre-equilibrated with dH2O and buffer I (25 mM HEPES/NaOH ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare), washed in lysis buffer and kept on beads in lysis buffer with 1 mM DTT at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... The eluted protein was concentrated and desalted in a PD-10 column (GE Healthcare) against a Tris 50 mM ...
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bioRxiv - Microbiology 2020Quote: ... Filtered lysate was used for protein purification with an Äkta start system (GE Healthcare). Lysis buffer containing 20 mM imidazole and wash buffer with 40 mM imidazole was used ...
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bioRxiv - Developmental Biology 2019Quote: ... The total lysate was pre-cleared with equilibrated Protein A Sepharose beads (GE Healthcare) for 2 hours at 4 degrees ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). StrepII-tagged proteins were detected using Strep-Tactin AP conjugate (IBA ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were visualized with the Amersham ECL Prime kit (GE Healthcare, Little Chalfont, UK). For membrane reprobing ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized via chemiluminescence using the Amersham Imager 600 (GE Healthcare Life Science). Quantification was performed using Aida Image Analyzer v4.26 (Raytest) ...
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bioRxiv - Cancer Biology 2021Quote: Refolded proteins were purified by anion exchange chromatography with Q Sepharose HP (GE Healthcare) column then Mono Q column (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... Uncleaved protein and protease were removed via reverse IMAC (1ml HisTRAP FF (GE Healthcare) followed by gel filtration (HiLoad® 16/600 Superdex® 75 pg (GE Healthcare)) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran, 0.45 μm; GE Healthcare Life Sciences) and incubated with primary α-GFP (monoclonal ...
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bioRxiv - Bioengineering 2021Quote: ... and IgG was purified with Protein A sepharose beads (GE Healthcare 17-5280-04).
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bioRxiv - Bioengineering 2021Quote: ... which was bound to 10 μL FastFlow Protein A Sepharose bead slurry (GE Healthcare) per 1e6 cells for 3 hours rotating at 4°C ...