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Citations for GE Life Sciences :
1951 - 2000 of 4633 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Free dye was separated from labelled protein by Illustra NICK columns (GE Healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were further purified by gel filtration using superdex 75 column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were further purified using a MonoQ 10/100 column (GE Healthcare) and eluted with a KCl salt gradient from 100 mM to 600 mM ...
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bioRxiv - Plant Biology 2019Quote: ... The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare) was incubated with the Arp2/3 complex in pull-down buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pathology 2020Quote: Immunoblotting was performed after protein transfer onto Hybond-P membranes (GE Healthcare, RPN303F). After blocking membrane nonspecific binding sites in TBS (20 mM Tris ...
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bioRxiv - Biophysics 2019Quote: ... Commercial NP protein (H3N2) was covalently immobilized on CM5 chips (GE Healthcare, USA) and used as a ligand ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) using film or the ChemiDoc Touch Imaging System (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow, GE Healthcare) and the purity and integrity were checked by SDS–PAGE ...
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by anion exchange chromatography (SOURCE 15Q, GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... bead-coupled proteins were incubated with PreScission Protease (GE Healthcare, 27-0843-01) in reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... and 700 μg protein was dissolved in 300 μL rehydration solution (GE Healthcare) for 1 hour at RT ...
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bioRxiv - Biophysics 2020Quote: ... The protein was further purified using a HiTrap Q HP column (GE Healthcare) for CTD-L and a HiTrap SP HP column for CTD with linear gradients of 50 to 500 mM NaCl ...
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bioRxiv - Immunology 2019Quote: ... Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow, GE Healthcare).
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bioRxiv - Synthetic Biology 2020Quote: ... Each protein was purified using a 1 mL HisTrap HP column (GE Healthcare), washing with 10 mM Hepes-NaOH ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were visualized by ECL chemiluminescence method using Amersham imager 600 (GE healthcare) and densitometry of protein bands was performed with ImageJ software.
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bioRxiv - Molecular Biology 2021Quote: ... The GST-proteins were purified using Glutathione Sepharose 4B (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... soluble proteins were loaded onto a Ni-column (HisTrap FF Crude, GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Expressed protein in supernatants were purified over a HisTrap excel column (GE Healthcare) and eluted in 500 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... the overexpressed protein was purified sequentially using HiTrap TALON crude column (GE Healthcare) and Superdex S200 26/60 column (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were purified using a 1 ml HisTrap HP column (GE Healthcare) or 5 ml GSTrap HP column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was loaded on a SP column (from GE healthcare Life sciences) and eluted by applying a NaCl gradient (from 0.15 to 1 M) ...
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bioRxiv - Cell Biology 2020Quote: ... The eluted protein was fractionated using a Superose10 gel filtration column (GE Healthcare). Protein concentrations were determined using a NanoDrop 1000 spectrophotometer (Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The GST-TTD fusion protein was purified using Glutathione sepharose 4B (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and the protein was bound to a HiTrap heparin HP column (GE Healthcare), before elution with a gradient from 335 mM to 1 M KCl ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 µL of Protein A Sepharose 4 FF (GE Healthcare Japan, Tokyo, Japan) was added and mixed with rotation at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Protein purification was performed using a 5 ml StrepTrap HP column (GE Healthcare) followed by SEC using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were purified sequentially by Ni-NTA affinity chromatography (HisTrap FF, GE Healthcare), heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Biochemistry 2021Quote: ... the expressed proteins were first purified by Glutathione Sepharose 4B beads (GE Healthcare), and then further purified with a HiLoad 16/600 Superdex 200 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was separated using a second HisTrap HP column (GE Healthcare). His6-tag free Ape1 protein was then loaded onto a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Phosphorylated proteins were recovered by gel filtration on Superdex 200 Increase (GE Healthcare) in 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were initially purified using Ni Sepharose 6 Fast Flow resin (GE Healthcare). The protein tags were cleaved with His-tagged TEV-protease overnight at 4 °C while dialyzing against TEV cleavage buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchanged protein was applied to a 5mL HiTrap QHP column (GE Healthcare,) using a 30% gradient of QB buffer (20mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated and loaded onto a Superose 6 column (GE Healthcare) pre-equilibrated in purification buffer and peak fractions were pooled and concentrated to 5-6 mg/mL.
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bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were visualized by chemiluminescent detection (ImageQuant LAS 4000, GE Healthcare Life Sciences) of peroxidase substrate activity (SuperSignal West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on SDS– polyacrylamide gel were transferred onto a nitrocellulose membrane (GE Healthcare). The bands were visualized utilizing Enhanced Chemiluminescence (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... and purified by fast protein liquid chromatography using Calmodulin-Sepharose (GE Healthcare, USA). TAT-CaM was expressed in BL21(DE3)pLysS E ...
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bioRxiv - Cell Biology 2021Quote: ... GST-fusion proteins were purified by incubation with glutathione-Sepharose beads(GE Healthcare). The precipitates were washed 3X with lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... Excess dye was removed by purifying protein over NAP-5 columns (GE Healthcare). Labeling was confirmed using NanoDrop 2000c (Thermo Fisher).
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bioRxiv - Microbiology 2020Quote: ... Spike protein was finally purified using Superose 6 HR10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES (pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... the protein solution was applied to a HiTrapTM Q HP column (GE Healthcare) and eluted with linear gradient of 0.15-2.0 M of NaCl in 10 mM Tris (pH 8.0) ...
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bioRxiv - Immunology 2020Quote: ... mRNA was purified by Fast Protein Liquid Chromatography (FPLC) (Akta Purifier, GE Healthcare), as described 74 ...
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bioRxiv - Immunology 2021Quote: ... by incubating sera with a resin of protein G Sepharose beads (GE Healthcare) for 2h ...
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bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by gel filtration chromatography (Superdex-200, GE Healthcare) with columns equilibrated in storage buffer (50 mM Tris-HCl 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to nitrocellulose membranes (GE Healthcare Life Sciences – REF 10600003), which were incubated with the appropriate antibodies according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... the reduced protein was bound to a Ni2+-Sepharose resin (GE Healthcare, UK) and washed with 10 column volumes of 50 mM KPi (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were wet transferred to nitrocellulose membrane (0.45 μm, GE Healthcare Life Sciences) at 100 V for 75 min ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were visualized using the ImageQuant LAS500 chemidocumenter (GE Healthcare, Chicago, USA). The ECL signal was visualized in the chemiluminescent channel ...
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bioRxiv - Cancer Biology 2022Quote: ... Buffer replacement in protein purification used Column PD 10 desalting column (GE Healthcare). All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue ...