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Citations for GE Life Sciences :
2351 - 2400 of 4633 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 20 µg of anti-HA monoclonal antibodies crosslinked to protein G MicroBeads (GE Healthcare) were added to the cleared lysate and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Synthetic Biology 2023Quote: ... Protein purification was performed with an ÄKTA start chromatography system (GE Healthcare Life Sciences) using a 5 mL HisTrap FF column (GE Healthcare Life Sciences ...
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bioRxiv - Genetics 2023Quote: ... Chromatin fragments bound to antibody were captured on Protein A-Sepharose beads (GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein complex was further purified over a Ni-charged chelating column (GE Healthcare) and eluted by a linear gradient to 500 mM imidazole ...
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bioRxiv - Neuroscience 2024Quote: ... the protein complex is purified by binding to a MonoQ Sepharose column (GE Healthcare) at 90 mM (sample dilution with 25 mM HEPES-KOH ...
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bioRxiv - Molecular Biology 2023Quote: ... nChIP’ed chromatin bound to Protein G Sepharose beads (GE Healthcare Cat #17-0618-02) were washed 3X with cold 0.5X PBS ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: Protein purification was carried out using the pGEX-6p1 (GE Healthcare, Little Chalfont, UK) bacterial expression vector ...
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bioRxiv - Molecular Biology 2024Quote: ... The total proteins were isolated and then purified using glutathione Sepharose 4B (GE Healthcare), Ni Sepharose (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto Amersham™ Hybond® P PVDF membranes (GE Healthcare, #10600023) and detected with the following antibodies ...
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bioRxiv - Biophysics 2024Quote: ... The labelled protein was purified on a Superdex 200 16/300 column (GE Healthcare) to separate it from the unreacted dye ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were loaded onto a Superdex 200 Increase 100/300 GL column (GE Healthcare) and the correct fractions were pooled ...
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bioRxiv - Cell Biology 2024Quote: ... Isolated proteins were applied to a Superdex 75 26/60 HiLoad column (GE Healthcare) equilibrated with buffer comprised of 20 mM Tris pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... which were conducted in the presence of Protein G Sepharose 4 beads (GE Healthcare). For phospho-CTD ChIP ...
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bioRxiv - Immunology 2024Quote: ... The proteins were transferred to Amersham Protran nitrocellulose blotting membrane (GE Healthcare Life Sciences) and further incubated with anti IRF7 (polyclonal antibody ...
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bioRxiv - Microbiology 2024Quote: ... 150 µL protein analysed on a Superdex 200 10/300 GL column (GE Healthcare) integrated in an NGC system via constant flow of SEC buffer with known monomeric proteins serving as calibration standards ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMC) were enriched by density gradient centrifugation of peripheral blood from healthy human donors through a Ficoll-Paque PLUS (GE Healthcare) gradient ...
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bioRxiv - Biochemistry 2019Quote: Human BRG1 AT-BD (residues 1434–1569) or BD (residues 1454-1569) were cloned into a pGEX6p-1 vector (GE Healthcare) from full length BRG1 cDNA obtained from GE Open Biosytems ...
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bioRxiv - Molecular Biology 2021Quote: ... The full-length human eRF3a was expressed in insect Sf21 cells and purified via affinity chromatography using a HisTrap HP column (GE Healthcare) followed by anion-exchange chromatography on a MonoQ column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Human PBMCs were isolated from leukopheresis packs obtained from the Central Blood Bank (Pittsburgh, PA) via Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... each well in the plate was further incubated with the HRP-sheep anti-mouse or anti-human secondary antibody (1:5000, GE Healthcare) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Microbiology 2021Quote: ... The primary antigen-antibody complex was detected by incubating the blot with goat anti-rabbit IgG HRP (1:10000, Jackson-ImmunoLab) and sheep anti-human IgG HRP (1:5000, GE Healthcare) in 3% milk in PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... lymphocytes and monocytes were isolated from heparinized human peripheral blood by Ficoll-PaqueTM PLUS (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) gradient centrifugation ...
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (Human SOX5, clone ID 30343519; mouse Rarb, clone ID 30608242; mouse Rxrg, clone ID 5707723; all purchased from GE Healthcare) were inserted into pCAGIG vector (Addgene plasmid # 11159) ...
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bioRxiv - Microbiology 2022Quote: ... siRNA (Catalog no. L-013607-00-0005) Human DHX9 (1660) siRNA (Catalog no. L-009950-00-0005) were from Ge Healthcare Dharmacon (Colorado ...
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bioRxiv - Biochemistry 2023Quote: The complementary DNA of human ZIP4 (GenBank access number: BC062625) and human ZIP8 (GenBank access number: BC012125) from Mammalian Gene Collection were purchased from GE Healthcare. The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Cell Biology 2023Quote: ... was constructed by inserting the cDNA for human SSH1 (869–1049) into the BamHI and NotI sites of pGEX-4T (GE Healthcare). E ...
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bioRxiv - Cancer Biology 2024Quote: ... were isolated from human buffy coats from the New York Blood Center by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare). Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were purified from buffy coats by two gradient centrifugations: PBMCs were initially isolated with Ficoll-Paque (GE Healthcare, Pittsburgh, PA) and subsequently peripheral monocytes were isolated with Percoll (GE Healthcare ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Immunology 2023Quote: FH was isolated from human plasma using an affinity column made up of a monoclonal antibody against human FH (MRCOX23; MRC Immunochemistry Unit, University of Oxford) coupled to CNBr-activated Sepharose (GE Healthcare, UK) as described previously (21) ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from heparinised blood and tissue derived mononuclear cells were isolated from mechanically dissociated and filtered human tissue using Ficoll density gradient centrifugation (Ficoll PaquePlus; GE Healthcare, Amersham) or 33% Percoll (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... The labelled protein was applied to a Thiopropyl Sepharose 6B column (GE Healthcare Life Sciences) to remove double labelled protein ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was then loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare), washed with 5 CV of Heparin Wash Buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The eluted protein was purified by Superose6 Increase 10/30 (GE Life Sciences, Cat. #29091596) gel filtration.
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bioRxiv - Biophysics 2020Quote: ... The protein solution was then loaded onto a Superdex 200 10/300 column (GE Healthcare). The peak fractions were pooled and concentrated (Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were passed over an either HisTrap or GstTrap FF 5□ml column (GE Healthcare), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Diluted proteins were subsequently passed over a HiTrap Heparin HP 5□ml column (GE Healthcare). After washing with binding buffer ...
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bioRxiv - Cell Biology 2019Quote: ... and immune complexes were captured using the Protein G Sepharose (17-0618-01, GE Healthcare). Samples for gel electrophoresis were prepared in Laemmli Sample Buffer (LDS ...