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Citations for GE Life Sciences :
2001 - 2050 of 4633 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Proteins were purified on a Ni-NTA HiTrap HP column (GE Life Sciences) and the His tag was removed by overnight cleavage with TEV protease (homemade) ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was desalted using a PD10 column (GE Healthcare, Cat. no. 17085101) in 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... NQO1 proteins were purified using immobilized nickel affinity chromatography columns (IMAC, GE Healthcare) as described (12) ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified by SEC on a Superdex 75 (GE Healthcare) column ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2,000 response units (RU) ...
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bioRxiv - Microbiology 2022Quote: ... The dimeric ACE2 protein was purified by GammaBind Plus Sepharose beads (GE Healthcare), followed gel filtration chromatography on a Superdex 200 Increase 10/300 GL column ...
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bioRxiv - Microbiology 2022Quote: ... Materials and equipment for protein purification were obtained from GE Healthcare (Munich, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... GST fusion proteins were immuno-precipitated using Glutathione Sepharose 4B beads (GE Healthcare). Fusion protein-bound beads were used directly in GST pull-down assays ...
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bioRxiv - Microbiology 2022Quote: ... protein was loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) with TBS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins separated on gels were electroblotted onto nitrocellulose (Protran 0.2 μm, GE Healthcare) with a constant current of 100 mA for 10 h using wet transfer ...
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bioRxiv - Plant Biology 2023Quote: ... the antibodies were further affinity purified using Protein G mag sepharose (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... the supernatants were precleared by incubating with protein G-Sepharose beads (GE Healthcare), and reacted with 100 μL of Strep-Tactin Sepharose beads (IBA Lifesciences ...
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bioRxiv - Molecular Biology 2022Quote: ... on an ÄKTA explorer fast protein liquid chromatography (FPLC) system (GE Life Sciences) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... were applied along with Protein G Sepharose 4 Fast Flow beads (GE Healthcare). All ChIP extracts were pre-cleaned for 1 hr with 30 μl beads on a rotor at 4°C in absence of the antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were purified from hybridoma supernatant using HiTrap Protein G columns (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... The separated proteins were transferred on Amersham Protran nitrocellulose membrane (GE Healthcare Lifescience). After blocking with 5 % skimmed milk in TBS-T (0.1 % Tween-20 (Sigma-Aldrich ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
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bioRxiv - Immunology 2022Quote: ... Protein bands were visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... The protein bands were detected using ECL (RPN2232, GE Healthcare, Little Chalfont, UK) on Amersham Imager 600 ...
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bioRxiv - Microbiology 2023Quote: ... The eluted protein was loaded on a HiTrap Q HP column (GE Healthcare) in buffer consisting of 20 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane (Whatman, GE Healthcare Life Sciences), which was blocked with 5% dry-milk-Tris Buffer saline ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was then reapplied to a GSTrap HP column (GE Healthcare), and the flow-through fraction was collected ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Each protein was purified on a Ni Sepharose High Performance column (GE Healthcare). The purified fractions were desalted and concentrated using an Amicon Ultra centrifugal filter unit (10-kDa cutoff ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were further purified using anion-exchange chromatography (Source 15Q, GE Healthcare) using a salt gradient from 0 to 1 M NaCl in a buffer containing 20 mM TrisHCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were pre-cleared with protein A-Agarose beads (GE Healthcare, Chicago, USA) for 1 h in a wheel shaker at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the proteins were transferred onto a nitrocellulose membrane (Hybond-C Extra, GE Healthcare) and an anti-His-HRP conjugate antibody (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... the proteins and domains were purified by ÄKTA Go System (GE Healthcare, USA) with Ni-NTA affinity chromatography column (HisTrap HP ...
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pADP-ribosylation Regulates the Cytoplasmic Localization, Cleavage and Pro-apoptotic Function of HuRbioRxiv - Cell Biology 2023Quote: ... were incubated with 60 µl of protein A-Sepharose slurry beads (GE Healthcare) (washed and equilibrated in cell lysis buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... mRNA was purified by Fast Protein Liquid Chromatography (FPLC) (Akta Purifier, GE Healthcare) [21] ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were wet transferred to nitrocellulose membrane (0.45 μM, GE Healthcare Life Sciences) at 100 V for 75 min ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was purified with Superose 6 Increase 10/300 column (GE Healthcare) (Fig ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was further purified on a Mono S cation exchange column (GE Healthcare) and stored in CHIP TPR storage buffer (10 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... A series of chromatography in a fast protein liquid chromatography system (GE Healthcare) was performed [31] ...
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bioRxiv - Microbiology 2023Quote: ... The purified proteins or domains were immobilized on CM5 chip (GE Healthcare, USA) at response unit 2914.6 ...
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bioRxiv - Microbiology 2023Quote: ... proteins were transferred to a 0.45 μm pore size nitrocellulose membrane (GE Healthcare) and incubated with rabbit polyclonal anti-Hfq antibody (Andrew Feig ...
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bioRxiv - Microbiology 2023Quote: ... IgG1 and IgG2 were purified using a HiTrap Protein A column (GE Healthcare) and Äkta Pure (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were further purified by size-exclusion chromatography (SEC) (Superdex 75, GE Healthcare) in a buffer consisting of 50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... N protein was then bound to an SP sepharose FF column (GE Healthcare) and eluted using a gradient of 0-100% buffer B (buffer A ...
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bioRxiv - Biophysics 2023Quote: ... N protein was then bound to an SP sepharose FF column (GE Healthcare) and eluted using a gradient of 0-100% buffer B (buffer A ...
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bioRxiv - Biophysics 2023Quote: ... The N protein was then run over a HisTrap FF column (GE Healthcare) in Buffer A (20 mM Tris pH 7 ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were transferred onto a 0.45μm nitrocellulose blotting membrane (#10600002; GE Healthcare) using wet electrotransfer for 1h at 110V ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from the lysate using Glutathione-sepharose 4B (GE Healthcare). His-tagged protein expression was induced by shifting to 15°C and adding 0.1 mM IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was injected into a Superdex 200 column (16/600; GE Healthcare) and eluted as a tetramer with the following buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred onto 0.2 μm pore size nitrocellulose membranes (GE Healthcare, 10600001) at 18 V for 1 h using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was then applied to a HiTrap Heparin HP column (GE Healthcare) which was then washed with 10 CV of Heparin Bind Buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was subjected to a desalting column (PD-10 Columns, GE Healthcare) to remove biotin ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were loaded onto a 1ml HiTrap Q HP column (GE Healthcare). Capping protein heterodimers were separated from other minor protein contaminants by linear gradient elution ...
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bioRxiv - Biochemistry 2023Quote: ... All chromatography steps were done in Fast Protein Liquid Chromatography systems (GE Healthcare).