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Citations for GE Life Sciences :
1751 - 1800 of 2341 citations for 3 Chloro 5 fluoro 4' morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Whole-brain images will be acquired at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison HealthEmotions Research Institute (Department of Psychiatry) using a 3 Tesla GE MR scanner (Discovery MR750; GE Healthcare, Chicago, IL, USA). A high-resolution T1 image will be acquired with a fast spoiled gradient recalled echo sequence (8.2 ms TR ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in 10 ml FACS wash and filtered through a nylon filter before being overlayed onto 3 ml Ficoll (GE Healthcare, 17-1440-03). Cell/Ficoll mixture was centrifuged at 931g for 1 hour at room temperature with brakes off ...
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bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed with TBS containing 0.1% Tween 20 (3 times for 15 min under shaking at RT) and probed with an anti-rabbit HRP conjugated antibody (NA934V, GE healthcare, Piscataway, NJ, USA) for 2 h at RT ...
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bioRxiv - Immunology 2024Quote: ... The cultures were started in a volume of 400 mL of basal medium at a cell density of 350,000-400,000 cells/mL and were supplemented with Cell Boost 7a (Hyclone) at 3% v/v and Cell Boost 7b (Hyclone, GE Healthcare, Chicago, IL, USA) at 0.3% v/v on days 0 ...
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bioRxiv - Cell Biology 2020Quote: ... The clarified lysate obtained after centrifugation (30 min at 20000 × g, 4°C) was first run on a streptavidin affinity column (StrepTrap® HP, GE Healthcare) equilibrated with septin 1 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Neuroscience 2021Quote: ... Co-IPs done to ckeck for homodimers were done the same way except that agarose beads (Protein G Sepharose™ 4 Fast Flow by GE Healthcare) were used that were incubated with mouse anti-myc antibody (9B11 ...
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bioRxiv - Biochemistry 2020Quote: ... 10(n)-3H-oleic acid (specific activity, 45.5 Ci/mmol) and [4-14C]-cholesterol (specific activity, 49.8 mCi/mmol) were purchased from GE Healthcare (Piscataway, NJ, USA). 1-14C-oleic acid was obtained from Moravek Biochemicals Inc ...
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bioRxiv - Plant Biology 2021Quote: ... Oligomeric states of OsbZIP47 protein was determined by analytical size exclusion chromatography (SEC) performed at 4°C on a Superdex 200 increase column (GE Healthcare Inc.) pre-equilibrated with buffer (25 mM sodium phosphate (pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... Size-exclusion chromatography was performed at 4 °C using a HiLoad™ Superdex 200pg 16/600 GE preparative column (GE Healthcare, USA). The column was equilibrated with 50 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Systems Biology 2021Quote: ... the gel was washed and exposed to phosphor screen (Fujifilm Bas cassette2, Japan) for 4 hours followed by imaging with Typhoon 9210 phosphorimager (GE Healthcare, USA).
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bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then centrifuged (21 K, 4°C, 30 min) and the supernatant applied to an AKTA Start (GE Life Sciences) at 5 mL min−1 ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged in a Beckman 45Ti rotor at 100,000 x g for 30 min at 4 °C and the supernatant applied to a HiLoad 26/10 Q Sepharose column (GE Life Sciences) equilibrated in 90 % buffer A and 10% buffer B (buffer A ...
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bioRxiv - Neuroscience 2024Quote: ... 20 μg of the heat-denatured protein lysate of each sample was loaded on a 4–12% SDS-PAGE and then transferred via wet blotting onto a 0.45 μm nitro-cellulose membrane (GE Healthcare, Solingen, Germany). After 1 h of blocking with 5% milk in TBST ...
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bioRxiv - Evolutionary Biology 2023Quote: Native PAGE with Laemmli buffer (pH 8.8) without SDS was performed in 4–20% gradient polyacrylamide gels in a miniVE Electrophoresis System (GE Healthcare, Life Science). High molecular weight standards (Pharmacia ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... The lysates were then centrifuged for 20 min at 45,000 g and 4 °C and the supernatants were filtered (0.45 μM) and loaded onto HisTrap HP columns (GE Healthcare, Uppsala, Sweden). Then ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2024Quote: Knockdown of KRAS was achieved by gene silencing using a pool of 4 small interfering RNAs (siRNA) specific for KRAS (ON-TARGETplus SMARTpool, from Dharmacon, GE Healthcare, USA). The desired cell line was plated onto a 6-well plate and allowed to adhere (150 000 cells per well) ...
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bioRxiv - Molecular Biology 2024Quote: ... The clarified lysate was incubated overnight at 4 °C with 1.5 mL of buffer A-equilibrated Glutathione Sepharose® 4B (GE Healthcare, US).
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bioRxiv - Molecular Biology 2024Quote: ... the gel was exposed to phosphor imager screen (Fujifilm Bas cassette2, Japan) for 4 h followed by imaging with Typhoon 9210 phosphor imager (GE Healthcare, USA) and Azure Sapphire (Azure Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun down at 14000 rpm at 4°C for 10 minutes and supernatant were incubated with GammaBind Sepharose (GE Healthcare, # 17088501) beads premixed with 1µg/mL rabbit α-mouse SorCS2 F7378 (DAKO custom-made polyclonal ab against the cytoplasmic tail of mouse SorCS2 ...
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bioRxiv - Immunology 2024Quote: ... using PEI-precipitation method and purified from culture supernatants by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) as previously described 15.
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bioRxiv - Biochemistry 2024Quote: ... apo-pirin was incubated overnight at 4°C with 1.2 equivalents of Fe(III) chloride hexahydrate and desalted on a PD SpinTrapTM G-25 (GE Healthcare, Chicago, Illinois) into pirin storage buffer ...
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bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mm x 1 mm x 1 mm) and FDG-PET data acquisitions were performed simultaneously on a hybrid 3 T SIGNA™ PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the Service of Nuclear Medicine (CUB Hôpital Erasme ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was then supplemented with 5 mM imidazole prior to loading onto a 1-ml HisTrap column (GE Healthcare) attached to an AKTA system at 1 ml/min ...
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bioRxiv - Microbiology 2021Quote: ... The resultant eluate was filtered through a 0.45 μm filtration membrane and then loaded onto a 5 ml SOURCE 15S column (GE Healthcare) equilibrated with buffer I ...
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bioRxiv - Biophysics 2020Quote: IgG was isolated from porcine sera by passing the sera over a MabSelect 5 mL HiTrap Column (GE Life Sciences), washing with two column volumes of tris-buffered saline and elution with 0.1 M glycine ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies have been conjugated to fluorochromes using commercially available kits according to the manufacturer’s instructions: anti-S100B has been conjugated to FITC (FLUKA) using Illustra NAP-5 columns (GE Healthcare), anti-GD2 (ch14:18 ...
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bioRxiv - Microbiology 2020Quote: ... The cleared membrane fraction was loaded with a flow of 0.8 mL/min on a pre-equilibrated 5 mL StrepTrap column (GE healthcare) mounted on as AktaPurifier system (GE healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... radioactive signal was impressed on a FUJIFILM Storage Phosphor screen for 5 hours and acquired with Typhon Trio (GE healthcare).
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bioRxiv - Biophysics 2022Quote: ... The RNA samples were first purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare, Chicago, IL) at pH 7.0 then annealed in buffers containing 40 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and the supernatant was loaded onto a 5-ml bed volume of HiTrap Heparin column (GE Healthcare, Pittsburgh, PA, USA) pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... reacted with 5 to 20-fold excess of Alexa-568 or Alexa-488 NHS ester (catalog # A20003, A20000, GE Healthcare) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... the sample was concentrated to 5 ml and loaded over the HiLoad 26/600 Superdex 200 prep grade (GE Healthcare). In order to clear the impurities which had been eluted with the complex ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was filtered through a 0.45 µm syringe filter and applied onto a 5 ml MBPtrap column (GE Healthcare). The column was washed with 10 CV of lysis buffer and the protein was eluted with 5 CV of lysis buffer supplemented with 15 mM maltose ...
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bioRxiv - Immunology 2021Quote: ... the supernatant of stable HEK293S cells expressing αMβ2 headpiece was recovered and purified by immobilized ion-affinity chromatography using a 5 mL HisTrap Excel column (GE Healthcare). The protein was subsequently applied to a 1 mL StrepTactin column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
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bioRxiv - Developmental Biology 2020Quote: ... the supernatant was boiled at 98 °C for 5 min, applied to SDS page gel (SuperSep, WAKO) and transferred onto a 0.2 µM nitrocellulose blotting membrane (GE Healthcare). After incubation with blocking buffer (5% milk in tris-buffered saline with 0.1% Tween20 ...
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bioRxiv - Cell Biology 2021Quote: ... and mCherry (5% laser power, 0,05 exposure) were recorded at 7 minutes intervals and data were analyzed using SoftWoRx (GE Healthcare). The time from nuclear envelope breakdown (NEBD ...
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bioRxiv - Genomics 2020Quote: ... 20 µl PCR products were subsequently immobilized to 5 µl Streptavidin Sepharose High Performance Bead (GE Healthcare, Piscataway, NJ, USA), and then were finally annealed to 1 µl sequencing primer (5 μM ...