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Citations for GE Life Sciences :
1701 - 1750 of 4074 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... A 1:1 combination mix of two of magnetic carboxylate beads was used (Sera-Mag Speed beads, #45152105050250, #65152105050250, GE Healthcare). Beads were prepared fresh by rinsing with water three times prior to use at a stock concentration of 20 μg/μL ...
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bioRxiv - Microbiology 2022Quote: ... Ten µg of a 1:1 mixture of hydrophilic and hydrophobic carboxyl-coated paramagnetic beads (SeraMag, #24152105050250 and #44152105050250, GE Healthcare) were added for each µg of protein ...
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bioRxiv - Cancer Biology 2022Quote: ... The binding affinity was determined by global fitting to a Langmuir 1:1 binding model within the Biacore Evaluation software (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was finally concentrated to 1–10 mg mL−1 using centrifugal concentrators with a cut-off at 100 kDa (Vivaspin 100, GE Healthcare), frozen in liquid nitrogen and stored at −80 °C in storage buffer containing 50 mM Mes-KOH (pH 7) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:3 and 3:1 (w/w) at a concentration of 1 mg/mL were separated on a Superdex 200 10/30 column (GE Healthcare) using a LC1100 HPLC System (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µL of a 1:1 mix of two types of commercially available carboxylate beads (Sera-Mag Speed beads, 45152105050250, 65152105050250, GE Healthcare), and 60 µL ethanol ...
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bioRxiv - Immunology 2022Quote: ... Synovial fluid mononuclear cells (SFMCs) and peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll Isopaque density gradient centrifugation (GE Healthcare Bio-Sciences, AB).
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bioRxiv - Immunology 2019Quote: ... Synovial fluid mononuclear cells (SFMCs) and peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll Isopaque density gradient centrifugation (GE Healthcare Bio-Sciences, AB) and frozen in Fetal Calf Serum (FCS ...
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bioRxiv - Immunology 2020Quote: ... Synovial fluid mononuclear cells (SFMCs) and peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll Isopaque density gradient centrifugation (GE Healthcare Bio-Sciences, AB) and were used after freezing in Fetal Calf Serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... at the i3S Advanced Light Microscopy Unit or with an IN Cell Analyzer 2000 equipped with IN Cell Investigator software (GE Healthcare, Little Chalfont, UK) at the i3S BioSciences Screening Unit ...
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bioRxiv - Immunology 2024Quote: ... Synovial fluid mononuclear cells (SFMCs) and peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll Isopaque density gradient centrifugation (GE Healthcare Bio-Sciences AB) and were used fresh or after freezing in FCS (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... coli cells were transformed with pGEX-4T-3 (GE Healthcare) containing the coding sequences of fascin ...
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bioRxiv - Cell Biology 2020Quote: Cells were imaged either on a DeltaVision system (Applied Precision) equipped with a PlanApo 60x/1.40 objective lens (Olympus) ...
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bioRxiv - Neuroscience 2021Quote: ... CFI/60 of the IN Cell Analyzer 2200 (GE Healthcare), a high-throughput imaging system equipped with a high-resolution 16-bit sCMOS camera (2048 × 2048 pixels) ...
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bioRxiv - Cancer Biology 2019Quote: ... Images were acquired by IN Cell Analyzer 2200 (GE Healthcare) with a 4x objective and quantitative image analyses were run in CellProfiler (www.cellprofiler.org ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were grown in HyClone PRMI medium (SH30027.02, GE Healthcare) supplemented with 10% GIBCO dialyzed FBS (26140-079 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were visualized using a DeltaVision microscope (Applied Precision Ltd.) fitted with a 60Å∼ ...
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bioRxiv - Plant Biology 2021Quote: ... Collected cells and performed purification using Ni-NTA (GE healthcare) affinity beads as previous described ...
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bioRxiv - Microbiology 2020Quote: ... using IN Cell Analyzer (version 6.2, GE Healthcare, Chicago, USA) and a 4x air objective (Nikon Plan Apo 0.20 NA ...
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bioRxiv - Neuroscience 2021Quote: ... Mononuclear cells were separated through Percoll (GE Healthcare Life Sciences) 37%/70% gradient centrifugation ...
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bioRxiv - Cell Biology 2019Quote: ... images were taken by IN Cell Analyzer 2000 (GE Healthcare), and then cells were classified into the S ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with HyClone Water (GE Healthcare Life Science) + 2% PFA for 20 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were mixed with either StrepTactin sepharose (GE Healthcare) for PorEStrep immunoprecipation ...
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bioRxiv - Immunology 2020Quote: ... and immune cells isolated using Ficoll-Plaque™ (GE Healthcare) gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... infiltrating immune cells collected by Percoll-gradient centrifugation (GE Healthcare) or ischemic brain tissues were lysed with RNAiso Plus (Takara) ...
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bioRxiv - Genetics 2021Quote: ... Mononuclear cells were obtained by Ficoll-Paque PLUS (GE Healthcare) density gradient isolation according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... Mononucleated cells were recovered by standard Ficoll-Hypaque (GE Healthcare) density-gradient centrifugation and CD34+ cells were isolated using anti-CD34 immunomagnetic beads (positive selection ...
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bioRxiv - Cell Biology 2022Quote: Cells were imaged either on a DeltaVision system (Applied Precision) equipped with a PlanApo 60x/1.40 objective lens (Olympus) ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were separated by Percoll (GE healthcare life sciences) discontinuous density centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were purified by a linear 40% Percoll (GE Healthcare) centrifugation step at 650 g without brakes for 25 min and the myelin top layer and supernatant were discarded ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellet was further resuspended in 30% Percoll (GE Healthcare) and spun at 300g for 15 min at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... Dead cells were removed using Ficoll-paque plus (GE Healthcare) and subsequently cultured in complete culture media supplemented with 5 nM human IL-2 (Peprotech ...
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bioRxiv - Molecular Biology 2023Quote: ... AD293 cells were cultured in DMEM/high Glucose (GE healthcare) supplemented with 10% (w/v ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected and spun over 20% Percoll (GE Healthcare). The cells at the interface were collected and cultured in poly-HEMA-coated flasks with StemSpan SFEM (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were purified by a linear 40% Percoll (GE Healthcare) centrifugation step at 650 g without brakes for 25 min and the myelin top layer and supernatant were discarded ...
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bioRxiv - Immunology 2023Quote: ... and immune cells isolated using a 30% Percoll (GE Healthcare) gradient ...
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bioRxiv - Neuroscience 2023Quote: ... CFI/60 of the IN Cell Analyzer 2200 (GE Healthcare), a high-throughput imaging system equipped with a high-resolution 16-bits sCMOS camera (2048×2048 pixels)32 ...
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bioRxiv - Immunology 2023Quote: ... peripheral blood cells were isolated by Ficoll gradient (GE Healthcare) and stained with an Ara h 2-Alexa Fluor 488 multimer and fluorescent antibodies ...
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bioRxiv - Immunology 2023Quote: ... and cells were enriched using a 35% Percoll (GE Healthcare) gradient.
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bioRxiv - Cell Biology 2023Quote: ... Cells were imaged using a DeltaVision Elite system (GE Healthcare) microscope equipped with ×40 (1.516 N.A ...
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bioRxiv - Microbiology 2024Quote: Cell-free supernatants were separated by Superdex 30 (GE Healthcare) size exclusion column equilibrated with distilled water and separated at 0.25 ml min-1 ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were then incubated with anti-BrdU (BU-1; 1:100; GE Healthcare, RPN202) and phospho-histone H2AX (Ser139 ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies coupled to horseradish peroxidase (HRP) were applied for 1 h: sheep anti-mouse (#NA9310V, GE Healthcare, 1:4000), donkey anti-rabbit (#NA9340V ...