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Citations for GE Life Sciences :
1801 - 1850 of 4074 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Appropriate secondary HRP-conjugated antibody was added for 1 hour at a dilution of 1:5000 followed by detection with ECL Prime Western Blotting Detection Reagent (GE Life Sciences; RPN2232) and exposure to autoradiography film at various time intervals or by digital imaging (LI-COR Odyssey) ...
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bioRxiv - Developmental Biology 2021Quote: ... horseradish peroxidase-conjugated antibodies at room temperature for 1 hr before exposure on photographic film (28906844, Amersham, UK) following incubation with ECL for 1 minute (RPN2232, GE Healthcare, IL, USA) as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... were freshly isolated from blood collected in EDTA-coated vials by layering diluted blood (1:1 in PBS) on top of an equal volume of Ficoll (GE Healthcare Life Sciences), followed by centrifugation and isolation of the buffy coat.
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bioRxiv - Microbiology 2022Quote: ... Protein samples were solubilized in DHHC Buffer C (1% SDS, 1 mM EDTA, 100 mM HEPES, pH 7.5) and were captured on thiopropyl sepharose 6B (GE Healthcare, 17-0402-01) in the presence of 2 M hydroxylamine or 2 M NaCl (negative control ...
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bioRxiv - Biochemistry 2020Quote: The human Cdc42 1-179aa Q61L (referred to as Cdc42)-encoding DNA was cloned into a modified pGEX-4T-1 vector (GE Healthcare, Chicago, Illinois) as previously described (Barthelmes et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Tryptic peptides were desalted using 500 µg of a 1:1 mixture of hydrophobic and hydrophilic Sera-Mag Carboxylate-modified Speed Beads (GE Healthcare Life Sciences).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with TBS + 0.1% Tween and incubated with goat anti-rabbit (1:2000, #NA934V) or anti-mouse (1:2000, #NA931V) secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Illinois) at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2020Quote: ... Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal) using an ECL kit (Amersham GE Healthcare Life Sciences) based on the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over Nap10 (GE Healthcare, 17-0854-01) and PD10 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over NAP- 10 (GE Healthcare, 17-0854-01) and PD-10 (GE Healthcare ...
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bioRxiv - Synthetic Biology 2021Quote: ... peripheral blood mononuclear cells were isolated by Ficoll-Paque (GE Healthcare) centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-16-cell embryos were transferred to 3 % Ficoll (GE Healthcare) in 0.6x MMR and injected with 5-10 nl of a solution containing mRNAs and/or MOs ...
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bioRxiv - Immunology 2020Quote: ... Cell culture medium containing 20 % FBS (SH30066.03, HyClone, GE Life Sciences), 10 mM HEPES (15630-080 ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell suspension was loaded onto a discontinuous Percoll (GE Healthcare) gradient (20% ...
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bioRxiv - Molecular Biology 2020Quote: ... Images were acquired using an IN Cell Analyzer 2200 (GE Healthcare), and quantitative image analyses were run in the open-source software CellProfiler (www.cellprofiler.org ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with the indicated siRNAs using DharmaFECT (GE healthcare), HiPerFect (Qiagen ...
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bioRxiv - Immunology 2019Quote: Splenic mononuclear cells were isolated by Ficoll-paque Plus (GE Healthcare) (Little Chalfont ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell were washed with phosphate-buffered saline (PBS, GE Life Sciences), scrape harvested ...
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bioRxiv - Microbiology 2020Quote: ... Individual infected cells were quantified using ImageQuant TL software (GE Healthcare). All pseudovirus work was performed in a Class II Biosafety Cabinet under BSL-2 conditions at Erasmus Medical Center.
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bioRxiv - Cancer Biology 2020Quote: ... plates were analysed using the IN Cell analyser 2000 (GE Healthcare) high-content analysis system ...
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bioRxiv - Cell Biology 2020Quote: ... T151-cells were cultured with tetracycline-free FBS (GE Healthcare, SH30070.03T)22 ...
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Secretin receptor as a target in gastrointestinal cancer: expression analysis and ligand developmentbioRxiv - Cancer Biology 2022Quote: ... Image processing was performed with IN Cell Investigator software (GE Healthcare), applying the provided granularity algorithm ...
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bioRxiv - Microbiology 2022Quote: ... The cell lysate was incubated with Ni-NTA-agarose (GE Healthcare) affinity resin for overnight at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Cells were imaged using a DeltaVision system (Applied Precision, Issaquah, WA) equipped with an inverted Olympus IX70 microscope (60X oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were incubated with 10 μl StrepTactin beads (GE Healthcare) for 45 min ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll (GE Healthcare) gradient and stored using standard protocols.
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bioRxiv - Neuroscience 2020Quote: Cells were imaged in a widefield fluorescence DeltaVision microscope (Applied Precision) equipped with a PlanApo 60x/1.40NA objective lens (Olympus) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were resuspended in one mL R10 (RPMI [GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... CFI/60 of the IN Cell Analyzer 2200 (GE Healthcare, Switzerland), a high-throughput imaging system equipped with a high-resolution 16-bits sCMOS camera (2048×2048 pixels) ...
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bioRxiv - Cell Biology 2019Quote: ... an IN Cell Analyzer 2000 automated imager (GE Healthcare, Chicago, IL), was used for sample imaging.
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bioRxiv - Molecular Biology 2019Quote: ... coli cells were transformed with pGEX6 bacterial expression constructs (GE Healthcare) harboring GST-SBP (streptavidin binding peptide)-RGG or the two KH domains from mouse FMRP ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were imaged on a DeltaVision Core system (Applied Precision) and centromeres were quantified with CRaQ as described (Bodor et al ...
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bioRxiv - Biochemistry 2019Quote: Cleared cell extracts were mixed with glutathione-Sepharose beads (GE Healthcare) conjugated to LC3 family proteins or GST ...
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bioRxiv - Molecular Biology 2020Quote: ... coli 55244 cells (ATCC) and purified using Protein L (GE Healthcare) gravity flow affinity chromatography as published previously29 ...
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bioRxiv - Immunology 2020Quote: ... FP was purified from cell supernatants using HisExcel column (GE Healthcare) and 1 ml Mono S column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in a 28% isotonic Percoll cushion (GE Healthcare) and centrifuged (900 g ...
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bioRxiv - Genomics 2021Quote: ... Mononuclear cells were isolated by ficoll density gradient centrifugation (GE Healthcare) and the buffy coats were collected ...
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bioRxiv - Neuroscience 2020Quote: ... After filtering cell pellets were resuspended in 40% Percoll (GE Healthcare) in HBSS ...
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bioRxiv - Molecular Biology 2021Quote: Cells were imaged using the DeltaVision System (Applied Precision, Issaquah, WA). Images were taken as z-stacks of 0.2-μm increments with an oil immersion objective (×100).
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bioRxiv - Immunology 2021Quote: ... The cell suspension was centrifuged on a Ficoll-Paque (GE Healthcare) cushion and the buffy coat layer was resuspended at a concentration of 1× 108 cells/ml in PBS + 2% FCS/ 1mM EDTA ...
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bioRxiv - Microbiology 2021Quote: ... Cells were viewed with a DeltaVision Core microscope system (Applied Precision) equipped with an environmental control chamber ...
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bioRxiv - Microbiology 2020Quote: ... Cell fractions were incubated with 120 µL streptavidin beads (GE Healthcare) overnight at 4°C with gentle agitation ...
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bioRxiv - Genomics 2022Quote: ... and mononuclear cells were isolated by Ficoll-Paque PLUS (GE Healthcare) gradient centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... Photos were taken using an IN Cell Analyzer 2000 (GE Healthcare) and PI incorporation was evaluated with the ImageJ software.
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bioRxiv - Molecular Biology 2022Quote: ... Images were acquired with IN Cell 2200 imaging system (GE Healthcare) and a custom CellProfiler pipeline was used for analysis (Data S3).