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Citations for GE Life Sciences :
1601 - 1650 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Neuroscience 2023Quote: ... A 20-μl droplet of virus inoculum containing 8X106 plaque-forming unit (PFU) of PRV-Becker, resuspended in medium (Dulbecco modified Eagle medium, 2% fetal calf serum and antibiotics) (Hyclone, GE Healthcare life sciences), was applied onto the abraded area of the skin ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Cell Biology 2024Quote: ... was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K. Basler, University of Zurich) and pGEX-6P-2 (GE Healthcare Life Sciences). Full length (FL ...
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bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...
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bioRxiv - Microbiology 2020Quote: ... The response data were fit to a 1:1 binding model using Biacore™ Insight evaluation software (GE Healthcare). For Fab competition experiments (Figure S8) ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody dilutions in 5% milk/PBST: anti-MBP HRP conjugated, 1:50,000 (NEB, and anti-GST HRP conjugates, 1:2,000 (GE Healthcare,). Scanned western blot films were quantified using the gel analysis tool in ImageJ.
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bioRxiv - Cancer Biology 2021Quote: ... and visualized using HRP-conjugated secondary antibodies (donkey anti-rabbit, 1:15,000, and sheep anti-mouse, 1:15,000, GE Healthcare) and ECL substrate (SuperSignal West Femto Maximum Sensitivity Substrate and Atto Ultimate Sensitivity Substrate ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then blocked for 1 h with TBS-T 1% ECL Prime blocking reagent (GE Healthcare cat # RPN418) at RT on an orbital shaker and blotted with primary antibody for 1 h at RT with gentle agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... blood was diluted 1:1 with PBS and layered on top of 15 mL of Ficoll-Paque (GE healthcare) in 50 mL conical tube ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). The equilibrium dissociation constants (binding affinity ...
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bioRxiv - Microbiology 2021Quote: ... Data were double reference-subtracted and fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Sensorgrams were fitted using 1:1 binding model and kinetic constants determined using Biacore T200 Evaluation software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). To determine the levels of nanobody competition with the human ACE2 and VHH72 of known epitope specificity ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was then incubated on a roller for 1 hr with 1 ml IgG Sepharose resin (GE Healthcare) that had been previously equilibrated in IgG Buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunostained embryos were mounted in clearing solution Omnipaque (350 mg litre−1 concentration per 1 ml iohexol, GE Healthcare) and imaged using Leica TCS SP8 DLS microscope as described above ...
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bioRxiv - Microbiology 2023Quote: ... and a 1×10 cm strip of Whatman Grade 1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA). As the filter paper was the only carbon source ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 h at room temperature with HRP-conjugated rabbit or mouse secondary antibodies (1:50,000, GE Healthcare). Enhanced chemiluminescence (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: 3D structured illumination microscopy of fixed T cells cells was performed with a commercial Deltavision OMXv4.0 BLAZE microscope (GE Healthcare, Amersham, UK) using a 60x ...
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bioRxiv - Cancer Biology 2023Quote: ... which was determined by imaging of DAPI stained cells using the Incell 2200 or 6500 Cell Imaging Systems (GE Healthcare, Parramatta, Australia).
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bioRxiv - Systems Biology 2020Quote: ... Cells were imaged using a DeltaVisionQR Elite (GE Healthcare), with a 60× 1.42NA Oil Plan APO and an InsightSSI 7 Colour Combined Unit illumination system (CFP = 438nm ...
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bioRxiv - Cell Biology 2022Quote: ... and mononuclear cell isolation on Ficoll-Paque (GE Healthcare) density gradient ...
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bioRxiv - Immunology 2021Quote: ... Cell pellets were resuspended in 37% Percoll (GE Healthcare) and centrifuged at 1200 x g for 30 min with deceleration set to 0 ...
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bioRxiv - Genomics 2019Quote: ... HEK293T cells were purchased from GE Life Sciences (HCL4517). K562 cells were purchased from Sigma-Aldrich (89121407) ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were imaged on a DeltaVision (Applied Precision) imaging system equipped with an IX71 microscope (Olympus) ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were isolated using Ficoll-Paque (GE Healthcare) and stored at −80°C.
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bioRxiv - Cell Biology 2021Quote: ... Cells were visualized using a DeltaVision microscope (Applied Precision) fitted with a 60X ...
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bioRxiv - Neuroscience 2019Quote: The GE IN Cell Analyzer 2500HS (GE Life Sciences) was used for imaging ...
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bioRxiv - Immunology 2021Quote: ... The cells were suspended in 40% Percoll (GE Healthcare). The suspension was overlaid on 70% Percoll and centrifuged at 836 g for 30 minutes at room temperature ...
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bioRxiv - Systems Biology 2021Quote: HEK 293 cells were cultivated in DMEM (GE Healthcare), supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... widefield high content imager (IN Cell 2200, GE Healthcare) using a 10x/0.45 NA lens and standard DAPI (nuclear stain ...
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bioRxiv - Microbiology 2022Quote: ... were taken using IN Cell Analyzer 2000 (GE Healthcare). HepG2 nuclei as well intracellular and extracellular sporozoites were counted using the IN Cell Developer Toolbox v.1.9.2 software (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were first reverse-transfected using DharmaFECT (GE Healthcare) and then transfected again with HiPerFect (QIAGEN ...
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bioRxiv - Neuroscience 2020Quote: ... cells were re-suspended in 37% Percoll (GE Healthcare) and centrifuged at 500g for 30min at 18°C ...
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bioRxiv - Immunology 2019Quote: ... Dead cells were removed by Ficoll centrifugation (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Cell pellets were resuspended in 44% Percoll (GE Healthcare) on an underlay of 67% Percoll ...
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bioRxiv - Microbiology 2021Quote: ... G1/swarmer cells were isolated using Percoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended into a 30% Percoll (GE Healthcare) solution and centrifuged to separate the fat from the cells ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated cells were resuspended in 40% percoll (GE Healthcare), overlaid onto 80% percoll and centrifuged at 500g (with brake off ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were imaged on a DeltaVision microscope (Applied Precision) using 100X oil immersion lens ...
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bioRxiv - Neuroscience 2024Quote: ... Pelleted cells were resuspended into 30% Percoll (GE Healthcare) and centrifuged at 700 × g for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... COS-7 cells were cultured in DMEM (GE Healthcare) supplemented with FCS (PAN-Biotech ...
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bioRxiv - Cancer Biology 2023Quote: ... mononuclear cells (BMMCs) were isolated by Ficoll (GE Healthcare) density gradient centrifugation and stored in liquid nitrogen until further use ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were incubated with Streptactin II (GE healthcare) affinity beads for 2 hours at 4°C while rotating ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were imaged with InCell Analyzer 6000 (GE Healthcare). Eight fields were captured from 2 wells ...
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bioRxiv - Cell Biology 2023Quote: ... and donkey anti-rabbit IgG (Cell signaling, GE Healthcare) used in a dilution of 1:10000 and incubated at room temperature for 2 h ...