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Citations for GE Life Sciences :
1501 - 1550 of 4074 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... and whole genome amplification was achieved by multiple displacement amplification with Phi29 DNA polymerase and the Illustra GenomiPhi v.2 kit (GE Healthcare, 25660032). Amplified genomic DNA (70 ng ...
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bioRxiv - Physiology 2019Quote: ... 20 islets per well (n = 3 biological replicates per group) were transfected with control (non-targeting #2) siRNA or mouse Brd4 SMARTpool siRNA (Dharmacon GE Life Sciences) at 100 nM each with lipofectamine 3000 according to the product instructions ...
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bioRxiv - Immunology 2019Quote: Total IgG antibodies were captured from 2 µL of plasma using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Immunology 2020Quote: SEC-purified SARS-CoV-2 RBD-SD1 was immobilized to a Ni-NTA sensor chip in a Biacore X100 (GE Life Sciences) to a response level of ~500 RUs ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA FPLC (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3 mL fractions ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 250 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA Purifier (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3-ml fractions ...
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bioRxiv - Molecular Biology 2021Quote: ... and analyzed (normalization, spot matching, expression analyses, and statistics) using Image Master 2-D Platinum 6 image analysis software (GE Healthcare, USA). Spots on the gel were identified and each spot was assigned a spot quantity (q) ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Angiography was also performed with a bolus intravenous injection of 2 ml/kg iohexol (Omnipaque™, 300 mg I/ml; GE Healthcare) using a CT-scanner (CT ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 W for 2–2.5 h) followed by detection on an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Life Sciences).
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bioRxiv - Synthetic Biology 2024Quote: ... the pellet was resuspended in 18 mL of MCB1 with 0.1% BSA and 2-4.5 mL of material was layered onto stepwise Percoll (GE Healthcare, Chicago, IL, USA) MCB1 gradients with 0.1% BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... data were fitted using a kinetic 1:1 binding model using the Biacore T200 Evaluation Software (GE Healthcare).
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bioRxiv - Bioengineering 2020Quote: ... Data were fitted using 1:1 Langmuir binding model within the Biacore 8K analysis software (GE Healthcare #29310604).
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bioRxiv - Cell Biology 2021Quote: ... and UNC- 16(1-506) was covalently coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... and the resulting data were fitted to a 1:1 binding model using BIAcore 8K Evaluation Software (v3.0.12.15655; GE Healthcare).
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Biochemistry 2019Quote: 1:1 v/v ratio of hydrophilic (conc. 10µg/µL; GE Life Sciences; cat. no. 4515-2105-050250) and hydrophobic Sera-Mag SpeedBeads carboxylate-modified magnetic beads (conc ...
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bioRxiv - Molecular Biology 2019Quote: ... Curves were fitted to a 1:1 binding model using the accompanying Biacore evaluation software (Biacore/GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or HRP-conjugated anti-mouse IgG secondary antibody (GE Healthcare, diluted 1:4000 in PBS with 1% BSA). Cells were then incubated in o-phenylenediamine (OPD ...
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bioRxiv - Bioengineering 2020Quote: ... Data were fitted with 1:1 Langmuir binding kinetic model using the Biacore 8K analysis software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... The resulting data were fit to a 1:1 binding model using the Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... 20 ug of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads, GE Life Sciences) were added to each sample in 50% ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... Data were fit with 1:1 Langmuir binding model within the Biacore 8K analysis software (GE Healthcare #29310604).
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bioRxiv - Immunology 2023Quote: ... Samples were further diluted with PBS (1:1) and separated using the Ficoll-Hypaque (GE Healthcare Life Sciences) density gradient (centrifugation 450g ...
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bioRxiv - Molecular Biology 2023Quote: ... treated 1 h before filming with siRDNA (1:1000) and imaged using a Deltavision Core system (Applied Precision).
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bioRxiv - Neuroscience 2023Quote: ... 20 μg of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads; GE Life Sciences) were added to each sample in 50% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction was loaded onto a 1-mL anion exchange column (HiTrap 1 mL HP, GE Healthcare) and purified on an Akta purifier system ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μg of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads; GE Life Sciences) were added to each sample in 50% ethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were filtered through a 30-μm cell strainer and single cells were resuspended in PBS (GE Healthcare Life Sciences, SH30256.01) with 0.1% BSA ...
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bioRxiv - Genetics 2020Quote: ... the microscopic images of the stained cells were obtained by a high-throughput automated image capturing system IN Cell Analyzer 2200 (GE Healthcare). The images were analyzed by a computer software CalMorph ...
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bioRxiv - Cell Biology 2020Quote: ... The majority of live-cell imaging for cells expressing CRIB-3mCitrine (see below for exceptions) was performed on a Deltavision Elite system (Applied Precision) with solid-state illumination ...
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bioRxiv - Immunology 2021Quote: ... Synovial fluid mononuclear cells (SFMCs) and peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque density gradient centrifugation (GE Healthcare) and were either used immediately or frozen and stored at -80°C until further use.
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bioRxiv - Neuroscience 2019Quote: ... The amount of Aβ42 bound to the cells was measured as mean fluorescent intensity using In Cell 6000 microscopy (GE healthcare, USA). The specific binding of Aβ42 was calculated by subtracting the fluorescence of cells in the absence of Aβ42 from that of fluorescence in the presence of varying concentrations of Aβ42 ...
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bioRxiv - Neuroscience 2021Quote: ... we performed automated quantification of neurite length using IN Cell Developer Toolbox analysis routines based on image stacks uploaded from the IN Cell Analyzer 6500 confocal imager (GE Healthcare). To accommodate treatment-induced variation of neurite morphology during image acquisition ...
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bioRxiv - Molecular Biology 2022Quote: ... Mononuclear cells (MNCs) were isolated from cord blood cells by centrifugation using Ficoll-PaqueTM PLUS (GE Healthcare Life Sciences, Buckinghamshire, UK). CD34+ cell enrichment was performed using EasySep™ Human CD34 Positive Selection Kit II (StemCell Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were released from G1 arrest by filtration wherein cells were captured on 1.2 μm filtration paper (Whatman® GE Healthcare), washed with 1 L YEPD and resuspended in the appropriate fresh media ...
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitation performed from whole cell extracts of NIH3T3-II10 and NIH3T3-K2 cells were following the same procedures with protein G Sepharose beads (GE Healthcare): 18 μg rabbit anti-TBPL2 (3024 ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were depleted by erythrocyte rosetting using sheep red blood cells and B cells were separated by Ficoll density gradient centrifugation as recommended by the manufacturer (GE Healthcare). The remaining erythrocytes were lysed in red blood cell lysis buffer (155 mM NH4Cl ...
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bioRxiv - Immunology 2021Quote: ... Digested tissue was filtered through 100-μm cell strainers and colon and lung cells were further enriched by 40% Percoll (GE Healthcare) centrifugation ...
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Cell Biology 2022Quote: ... The fluorescence levels of Cyclin B1 in HeLa cells treated with nocodazole were measured using the IN Cell Developer Toolbox software (GE Healthcare). After background subtraction ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were seeded at ∼ 7.93 × 108 total viable cells (around 7.93 × 105 viable cells/mL) in a chemically defined media (ActiPro media; GE Healthcare, USA). The cultures were maintained with setpoints for pH at 7.2±0.05 ...
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bioRxiv - Molecular Biology 2023Quote: ... The medium was changed one day after transfection and live cells were imaged on an IN Cell Analyzer 2200 (GE Healthcare) at 4x magnification before the plate was returned to the incubator and cells were allowed to expand ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) or polymorphonuclear cells (PMNs) were isolated from whole blood by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare) or Polymorphprep (Axis-Shield) ...
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bioRxiv - Cell Biology 2020Quote: ... pGEX-6P-1 (GE Healthcare Life Sciences).
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bioRxiv - Genomics 2021Quote: ... using peristaltic pump P-1 (GE Healthcare) at a flow rate of 2 ml/min ...
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bioRxiv - Immunology 2022Quote: ... and anti-mouse (1:25,000; GE healthcare) IgG secondary antibodies ...