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Citations for GE Life Sciences :
1601 - 1650 of 5304 citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The ÄKTA Pure Protein Purification system was purchased from GE Healthcare. The microplate reader was purchased from Bio Tek.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified using a HisTrap HP column (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... 100 mM NaCl for all proteins with PD10 columns (GE Healthcare) and protein concentrations were determined by the Bradford assay (Biorad) ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Separated protein samples were transferred to a nitrocellulose membrane (GE Healthcare). Following transfer ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were subsequently purified over a heparin column (GE Healthcare). Complexes were eluted by a linear gradient to 1 M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred and immobilized onto a nitrocellulose membrane (GE Healthcare) by electrophoresis for 1h at 100V in standard transfer buffer containing 20% methanol ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were transferred onto 0.45 μm nitrocellulose membranes (GE healthcare). The membranes were blocked with 5% non-fat milk diluted in PBS 1X (Euromedex ...
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bioRxiv - Cancer Biology 2024Quote: ... Separated proteins were transferred to Hybond™ nitrocellulose membranes (GE Healthcare) using a semi-dry method (Bio-Rad Trans-Blot® Turbo™ Transfer System) ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant proteins were purified by glutathione Sepharose 4B (GE Healthcare) or Amylose Resin High Flow (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... Protein purification was conducted using a Resource Q column (GE Healthcare), eluting TET2 using a linear gradient of Elution buffer over 10 column volumes ...
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bioRxiv - Synthetic Biology 2023Quote: ... recombinant proteins were purified using Ni-Sepharose affinity chromatography (GE Healthcare). Final elution was carried out at 250 mM imidazole ...
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bioRxiv - Synthetic Biology 2023Quote: ... An AKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare) containing a Ni-Sepharose affinity chromatography (HisTrap HP ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were buffered in PBS using NAP-5 columns (GE healthcare) or Zeba spin columns (Thermo Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membranes (GE Healthcare, USA), and then probed with primary antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... Eluted proteins were desalted using a Q10/ PD10 column (GE Healthcare) and quantified using a Bradford ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by centrifugation (19,000 × g, 15 min, 4°C) was applied to a His SpinTrap column (GE Healthcare) previously equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Cell lysates were centrifuged at 75,000 x g for 25 min and supernatants were loaded on a GSTrap FF 1 mL column (GE Healthcare) using an AKTA Start purification system (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was recovered by centrifugation (50,000 g, 20 min, 4°C) and applied to a HisTrap FF NiNTA column (GE Healthcare). After washing with buffer A (50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was then centrifuged at 18,800 × g for 10 min and the resulting supernatant was applied to His Spin Trap (GE Healthcare). After centrifugation (100 × g ...
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bioRxiv - Biophysics 2019Quote: ... The bacterial lysate was clarified using centrifugation at 20,000 x g for 40 min and loaded onto a 5 ml His-Trap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Physiology 2020Quote: ... Cell pellets were resuspended with Medium 199 X1 and cells subsequently separated using a three layer Percoll density gradient (1.028, 1.05 and 1.088 g/ml) according to manufacturer instructions (Percoll plus, GE Healthcare Life Sciences) and as described previously83 by centrifuging at 600g for 30 minutes with controlled acceleration and braking ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by centrifugation at 25,000 × g for 30 min and were then bound to Ni-NTA beads (GE Healthcare). After extend washing with buffer containing 50 mM imidazole ...
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bioRxiv - Cell Biology 2019Quote: ... ultra-centrifuged (280.000 x g, 10 min, 4°C) and gel-filtered using a Superose6 10/300 GL column (GE Healthcare). Peak fractions were pooled ...
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bioRxiv - Cell Biology 2019Quote: ... The extract was pre-cleared by centrifugation at 20.000 x g for 45 minutes and the supernatant was incubated for 1 hr with Streptactin Sepharose beads (GE Healthcare) which had been washed 3 times in the lysis buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... the lysate spun down at 12,000 x g for 30 min and then loaded onto a 5 ml His-Trap Crude FF column (GE Healthcare) using a ÄKTA Purifier protein purification system (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... which is complementary to the tracrRNA 5’-end was labeled with ATTO647N-NHS (1:60 DNA:dye molar ratio) and purified using G-25 micro spin column (Illustra, GE Healthcare). The measured labeling efficiency of ATTO647N-oligonucleotide was >80% ...
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bioRxiv - Biochemistry 2020Quote: ... for 30 minutes with or without cofactor GMPPNP (30 μM) then applying 0.5 mL to a PD-10 Sephadex G-25 gel-filtration column (GE Healthcare). Eluted fractions (0.5 mL ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 48,284 g for 45 min at 4°C and loaded into a HisTrap FF cartridge (GE Healthcare). Proteins were eluted with 100 mM HEPES ...
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bioRxiv - Bioengineering 2020Quote: ... and 35 ml of this suspension was carefully added to 15 ml Ficol-Paque PLUS (1.077 g cm-3, GE Healthcare) and centrifuged at RT ...
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bioRxiv - Biochemistry 2020Quote: ... reactions were ended with 30 mM EDTA and unincorporated nucleotides were removed using Illustra MicroSpin G-50 columns (GE Healthcare). Samples were then separated on 0.6% alkaline agarose gels (30 mM NaOH and 2 mM EDTA ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was centrifuged at 38,000 ×g and the supernatant was loaded onto a 5 mL HisTrap Ni-NTA column (GE Healthcare). The column was washed with HisTrap Buffer and protein eluted with a linear gradient of imidazole from 25 to 300 mM in HisTrap Buffer over 10 column volumes.
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 30 min at 30.000x g at 4 °C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mmol L−1 Hepes at pH 8 ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane extract was centrifuged for 35 mins at 200,000 x g and the supernatant loaded onto a 10 ml Nickel column (Chelating Sepharose; GE Healthcare) equilibrated in TSB with 0.2% DDM pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... with a JA30.50 Ti rotor at 20,000 ×g and applied to a HiTrap SP HP column (GE Healthcare; 1 ml) equilibrated in buffer A (20 mM Na-phosphate buffer (pH 7.0) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation for 1 hour at 38,700 x g and passed over a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 15 column volumes of 20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was centrifuged at 20,000 x g and the supernatant was loaded onto a StrepTrap HP column (GE Healthcare). Protein was eluted in binding buffer with 2.5 mM D-desthiobiotin ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble material was sedimented by centrifugation at 13,500 g for 30 min at 4°C prior to loading on His-trap FF columns (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated to 0.5 ml and after centrifugation at 21,700 g for 20 min directly applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in SEC buffer (50 mM TRIS pH 7.8 ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysate was centrifuged at 45,000 x g for 35 minutes and supernatant was loaded onto a HisTrap HP affinity column (GE Healthcare). A linear gradient of buffer B (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Fractions between 8.5 and 10 mL were pooled and concentrated by centrifugation at 350 x g using Vivaspin filters with a 10,000 kDa molecular weight cutoff (GE Healthcare). Concentrated GPC-I53-50NPs were then diluted 1:1 with TBS ...
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bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant after centrifugation at 17,000 g at 4 °C for 1 hr was incubated with Glutathione Sepharose 4B (GE healthcare) at 4 °C for 3 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell debris and unlysed cells were pelleted at 48,000 × g and the supernatant was incubated with glutathione beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... supernatant was adjusted to pH=7 and loaded onto a High-Trap Prot G column (GE Healthcare, Chicago, Illinois, USA) using an Äkta system (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... After centrifugation at 60,000 × g the supernatant was used for purification using nickel-affinity chromatography on a HisTrap HP column (GE Healthcare), using an isocratic elution of low to high imidazole buffer (500 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: Protein samples concentrated to ∼1.0 g/L were injected into an HPLC-driven SEC column (Superdex 200 10/30 column, GE Healthcare) equilibrated with HBS-C buffer ...