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Citations for GE Life Sciences :
1401 - 1450 of 5304 citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Proteins were revealed by mean of enhanced chemiluminescence (GE Healthcare) in a BioRad ChemiDoc XRS+ ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was eluted using an AKTA FPLC (GE healthcare) and again checked for purity using SDS-PAGE.
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bioRxiv - Molecular Biology 2024Quote: ... proteins were electroblotted to PVDF membrane (0.2 μM, GE Healthcare) for 1 h at 100 V in ice-cold Bolt™Transfer Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated proteins were captured with Streptavidin Sepharose beads (GE Healthcare) and bound proteins analyzed by mass spectrometry.
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Glutathione Sepharose 4B (GE Healthcare). GST was cleaved from protein by TEV protease treatment for 16 hours at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The labeled proteins were purified with a PD10 (GE Healthcare) gel filtration column pre- equilibrated with Storage buffers (hSSB1 ...
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bioRxiv - Biochemistry 2024Quote: ... The labeled protein was purified with a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer and dialyzed against storage buffer to remove residual free dye.
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bioRxiv - Biochemistry 2024Quote: ... the labeled protein was purified using a PD10 (GE Healthcare) gel filtration column pre- equilibrated with INIP Storage buffer (25 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred to nitrocellulose membranes (GE Healthcare). For immunoprecipitations ...
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times for 10 min in TBS-T and incubated with the secondary horse radish peroxidase (HRP) coupled antibody (GE Healthcare, NXA931V; in a 1:3000 dilution) for two hours under agitation at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... Counter-exchange substrate not incorporated into proteoliposomes was removed via gel filtration on Sephadex G-25M columns (GE Healthcare). Transport assays were started either by adding 0.2 mM [α-32P]-phosphoric acid (6,000 Ci/mmol ...
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bioRxiv - Genetics 2021Quote: ... Radioactive labelled oligonucleotide probes were purified with Illustra MicroSpin G-25 micro columns (GE Healthcare, Chalfont St Giles, UK).
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bioRxiv - Immunology 2019Quote: ... anti-mouse immunoglobulin (Ig)-G horseradish peroxidase (HRP)-linked secondary antibody (Amersham catalogue number NA931, GE Healthcare, Buckinghamshire UK) diluted 1:4000 was incubated at RT for 2 hours before final washes and 3,3’,5,5’-Tetramethylbenzidine (TMB ...
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bioRxiv - Microbiology 2019Quote: ... the preparation was diluted in PBS and applied to a Sephadex G-25 column in PD-10 (GE Healthcare). Particle concentration and size distribution of the liposomes generated were evaluated using the NanoSight NS300 instrument (Malvern ...
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bioRxiv - Plant Biology 2020Quote: ... The lysate was cleared by centrifugation at 50,000 x g and filtered through a 0.45 µm filter before application to a HisTrap FF column (GE Healthcare). The column was washed with IMAC buffer (20 mM TRIS/HCl (pH 8.0) ...
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bioRxiv - Genomics 2021Quote: ... DNA from the isolated pooled LL and HL fractions was desalted on PD MiniTrap G-25 columns (GE Healthcare) equilibrated in 10m Tris-Cl pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity was detected using a horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (IgG, 1:10,000, NA934 GE Healthcare). Tau13 and Tau5 immunoreactivity were detected using an anti-mouse IgG (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... labelled probe was purified by native TBE-PAGE gel and illustra G-25 columns (GE Healthcare, Cat. No. 27532501). Equal intensity of different RNA probes were mixed with equal molar of different C/EBPα proteins individually in EMSA buffer B1 (Active Motif ...
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bioRxiv - Neuroscience 2021Quote: ... The mixture was clarified by centrifugation at 90,000 x g and the supernatant was added to 0.5 mL StrepTactin Sepharose resin (GE Healthcare) per gram of cells and rotated at 4°C for 2 h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Unincorporated γ-32P ATP was removed by running the product through Illustra MicroSpin™ G-25 Columns (GE Healthcare) and probe radioactivity was measured.
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Biochemistry 2020Quote: ... The conjugated Fab were then eluted from a PBS pre-equilibrated PD-mini G-25 desalting column (GE Healthcare) that removed unconjugated dye ...
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bioRxiv - Biochemistry 2021Quote: ... The ACP-TNB− was isolated and desalted using a Sephadex G-25 PD-10 desalting column (GE Healthcare, #17085101) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Microbiology 2020Quote: ... after which the supernatants were desalted on a Sephadex G-25 column (Illustra NAP-10 Columns, GE Healthcare, Pittsburgh) and concentrated by rotary evaporation ...
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bioRxiv - Microbiology 2021Quote: ... Following centrifugation at 18000 ×g the soluble fraction was applied to a 5 ml HisTrap HP column (GE Healthcare). The column was washed and protein eluted along a gradient using 20 mM Tris pH8 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies and associated DNA were then isolated using Gammabind G Sepharose beads (GE Healthcare Bio, catalog # 17-0885-01). Crosslinks were reversed by incubating samples and input at 65°C for at least 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... the biotinylated bait oligonuleotides were purified using Microspin Sephadex G-50 columns according to the manufacturer’s instructions (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... clarified 400,000 x g for 30 minutes and applied to a Superdex 200 Increase 10/300 GL (GE Healthcare) gel filtration column equilibrated in G-buffer containing 0.2 M ammonium acetate ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess of DCP-bio1 was removed from the collected supernatant by filtration on Sephadex G-25 columns (GE HealthCare). The resulting samples were quantified using BCA and a minimum of 50µg of proteins were subjected to NeutrAvidin pull-down as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Microbiology 2023Quote: ... the labelled oligonucleotides were separated from free [γ-32P] ATP using a custom Sephadex G-25 column (GE Healthcare). Labelled nucleic acids were incubated with nucleases (Rnase A ...
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bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
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bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
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bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
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bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Human PBMCs were isolated from the blood by density gradient using Percoll (GE Healthcare, 17-5445-01). PBMCs were washed and resuspended in RPMI 1640 supplemented with 10% FBS.
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare, Cat#17-5442-03). B cells were purified by negative selection (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were obtained from buffy coat by density gradient centrifugation in Ficoll–Paque PLUS (GE Healthcare). PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (GE healthcare). The membrane was blocked with 5% non-fat dry milk in PBS containing 0.1% v/v Tween 20 (PBST ...
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bioRxiv - Cancer Biology 2021Quote: ... GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare) by rotating in binding buffer (50 mM Tris HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were electrotransferred onto a 0.2 μm nitrocelluose membrane (GE Healthcare) and blocked with 5 % skim milk in 0.1 % Triton in Tris Buffer Saline (T-TBS ...
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bioRxiv - Cell Biology 2020Quote: ... For western blotting proteins were transferred on nitrocellulose membrane (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Protein input was detected using an anti-GST antibody (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The labelled proteins were then visualized using ECL prime (GE Healthcare) and LAS3000 (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then transferred to Hybond-P PVDF membrane (GE Healthcare). Immuno-staining was performed with 1/100 anti-MYD88 antibody (ab2064 ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred and immobilized onto a nitrocellulose membrane (GE Healthcare) by electrophoresis for 2 h at RT in a standard transfer buffer containing 20% methanol ...