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Citations for GE Life Sciences :
1701 - 1750 of 5304 citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... monkey (XP_005545315.1; 121-760aa) and human (NP_001121620.1; 120-760aa) TfR1 in 0.1% BSA in HBS-EP+ buffer (GE Healthcare) was captured at flow rate 10 μl/min ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated from human CMV-seropositive buffy coats by density centrifugation over Ficoll Paque Plus (GE Healthcare) and screened for the presence of NKG2C+ NK cell expansions by flow cytometry 51 ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA encoding human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare, and inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Microbiology 2021Quote: ... and SARS2-38 mAb proteins were produced in CHOEXPI or EXPI293F cells and affinity purified using HiTrap Protein A columns (GE Healthcare, HiTrap mAb select Xtra #28-4082-61). Purified mAbs were suspended into 20 mM histidine ...
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bioRxiv - Plant Biology 2021Quote: ... 0.5 ml aliquots of xylem sap were filtered through Sephadex columns (PD SpinTrap® G-25, GE Healthcare, Chicago, Il, USA) using Tris-buffer (50 mM Tris HCl (Roth) ...
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bioRxiv - Biochemistry 2021Quote: ... All labeled and unlabeled G-actins were further purified by size-exclusion chromatography on Sephacryl S200-HR (GE Healthcare, Chicago, IL), stored on ice in G-buffer ...
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bioRxiv - Cell Biology 2020Quote: Proteins (30 to 50μg of total cell lysates) were separated in SDS-PAGE gels and transferred to PVDF membranes (Amersham Hybond-P, GE Healthcare). Membranes were then blocked in PBS-T (PBS – 0.1% Tween 20 ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... After two centrifugation steps at 3000 x g to remove cell debris the AAV was isolated from the supernatant using heparin columns (GE Healthcare). After loading the column with the virus particles and washing with buffers containing 20 mM Tris and increasing concentrations of NaCl (100 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then cleared by centrifugation (18000 g, 4 °C, 1 hour) and purified using a HisTrap HP 5 ml column (GE Healthcare) and a 10-100 mM imidazole linear gradient followed by elution with 250 mM imidazole ...
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bioRxiv - Genomics 2020Quote: ... annealed with complementary strands to make double-stranded oligos and purified using G-25 spin columns (GE Healthcare, #27-5325-01). Binding reactions were set up as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Cytiva #SH30243.02) supplemented with 10% fetal bovine serum (GE Life Sciences #SH30910.03), 2 mM L-Glutamine (GE Life Sciences #SH30852.01) ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was clarified by centrifugation at 32000 g for 30 min and the supernatant was loaded onto HisTrap HP column (GE Healthcare). The column was washed extensively with buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... dynein constructs were mixed with 20 μM Halo Alexa488 dye and incubated on ice for 10 min and a PD MiniTrap G-25 column (GE Healthcare) equilibrated with dynein gel filtration buffer was used to remove excess dye afterwards.
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was heat-inactivated for 5 min at 95°C and the free nucleotides were removed on a Sephadex G-25 column (GE Healthcare). The oligo was annealed to a 1.5x excess of the unlabelled complementary strand by heating for 5 min to 80°C and slowly cooling to RT in annealing buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2019Quote: ... centrifuged for 10 min at 5000 g and the lysate was passed through a Source-15Q anion exchange 55 ml column (GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 12000 g at 4 °C for 1 h and loaded onto a HisTrap HP column (GE Healthcare) equilibrated with 98% buffer A/2% buffer B (20 mM Tris ...
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bioRxiv - Bioengineering 2021Quote: ... which exhibits a dissociation binding constant approaching attomolar concentrations (KD~10−18 M).[20] EDTA was added to formulations (4 eq with respect to zinc) to sequester zinc from the formulation and then lispro was isolated using PD MidiTrap G-10 gravity columns (GE Healthcare) to buffer exchange into water ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation at 18000*g for 45 min at 4 °C and the supernatant was applied to a Ni2+–Sepharose 6 Fast Flow (GE Healthcare) loaded gravity column ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were clarified by centrifugation (20 minutes at 18,000x g) and bound to a 5 mL HisTrap™ FF Crude column (GE Healthcare) and eluted with 50-75 mM imidazole ...
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bioRxiv - Biophysics 2021Quote: ... The flow through containing SARS-CoV-2 Mpro was collected and further purified using size exclusion chromatography column (G-100, GE Healthcare,) equilibrated with 20 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation (40000 g for 30 min) and the supernatant applied onto a 5 ml StrepTrap column (GE Healthcare). After washing with 10 column volumes (CV ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl) of lgG Sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... was filtered using a 100 kDa molecular weight cut-off (mwco) tangential flow filter (A/G Technology, Amersham Biosciences, GE Healthcare) and viruses and cells were concentrated to about 2 liters ...
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bioRxiv - Biophysics 2020Quote: ... Plexins and GTPases were further purified by either size exclusion chromatography or using a PD-10 Sephadex G-25 desalting column (GE Healthcare) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Insoluble material was removed by ultracentrifugation at 150,000 x g for 20 min before purification by SEC on a Superpose 6 increase 10/300 GL column (GE Healthcare) equilibrated with buffer (25 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... and the excess of dye was removed by using a PD Minitrap G-25 column for NPM or a Superdex 200 10/300 GL (GE Healthcare) size exclusion chromatography column for SET/TAF-Iβ ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing the enzyme were pooled and applied to a Sephadex™ G-25 (medium) (GE Healthcare, Piscataway, NJ, USA) column equilibrated and eluted in 50 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: Human adult peripheral blood mononuclear cells (PBMCs) were purified by density centrifugation using Ficoll-Paque (density = 1.077 g/mL; 600g, 30 minutes; GE Healthcare; USA). Informed written consent was given by donors to give approval for the use of waste material for research purposes ...
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bioRxiv - Developmental Biology 2022Quote: ... in presence of 32P end-labeled telomeric primers that has been purified using a micro-spin G-25 column (GE healthcare). PCR reactions were resolved by 9% polyacrylamide gel electrophoresis at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted at 3500 g and the media containing secreted zRETECD was pooled and 1 ml of IgG sepharose resin (GE Healthcare) was added to 1 l of media and incubated at 4 °C for 18 hrs ...
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bioRxiv - Immunology 2022Quote: ... and cells were resuspended in PBS-EDTA (room temperature) and layered onto Ficoll Paque (1.077 g/mL; GE Healthcare Europe GmbH). After centrifugation (800 x g ...
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bioRxiv - Biophysics 2019Quote: ... 60 - 120 µL of extruded sample were buffer-exchanged through 1.5- to 3.0-mL Sephadex G-50 Fine resin (GE Healthcare, Chicago, IL) columns equilibrated with buffer F (Table 2) ...
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bioRxiv - Plant Biology 2019Quote: ... and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated in a rotator overnight at 4°C and the immunocomplexes were recovered with FA buffer pre-equilibrated SPA/G beads (GE Healthcare) by rotating 2h at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then again centrifuged for 10 minutes at 400 × g and the resulting pellet was resuspended in HBSS before layered on top of a phosphate buffered percoll (GE Healthcare) gradient with concentrations of 85 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The reaction was quenched by the addition of 20 μl water followed by desalting in a MicroSpin G-25 spin column (GE Healthcare). The ∼53 μl eluate was transferred to a PCR tube and placed under a 385 nm UV lamp (∼3 cm distance from the tops of the tubes ...
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bioRxiv - Biochemistry 2020Quote: ... All reactions were stopped using 30 mM EDTA and unincorporated nucleotides were removed using Illustra MicroSpin G-50 columns (GE healthcare). Samples were run through 0.8 % alkaline agarose gels including 30 mM NaOH and 2 mM EDTA for 16 hours at 24 V ...
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bioRxiv - Pathology 2021Quote: ... After this time excess TCEP was removed by diafiltration into fresh BBS buffer using PD Minitrap G-25 columns (GE Healthcare) and the concentration was corrected to 20 μM ...
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bioRxiv - Physiology 2021Quote: ... The hemolysate was then centrifuged at 4°C (12000 g, 20 min) to remove cell debris and then passed (‘stripped’) on a PD-10 desalting column (GE Healthcare) equilibrated with 10 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation for 1 hour at 60000 x g and then loaded on a 5 mL Histrap FF column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation on Ficoll-Paque™ PLUS (1.077 g/L; GE healthcare). Then ...
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bioRxiv - Genomics 2019Quote: 10 μg of total RNA were loaded on denaturing 1.2% agarose gel and transferred to Hybond™-XL nylon membrane (GE Healthcare). 32P-labelled oligonucleotides (see Table S4 ...
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bioRxiv - Cell Biology 2019Quote: ... the antibodies were separated from unconjugated dye by gravity filtration through Sephadex G-25 DNA grade size exclusion columns (GE Healthcare) by visual detection ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was inactivated at 75 °C for 10 min and the labeled RNA/DNA was purified by gel filtration (Illustra MicroSpin G-25 columns; GE Healthcare). Recombinant ...
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bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
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bioRxiv - Biochemistry 2021Quote: ... A 30 μl aliquot of a given reaction was quenched by the addition of 20 μl water followed by desalting in a MicroSpin G-25 spin column (GE Healthcare). The NPOM (caged ...
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bioRxiv - Cancer Biology 2020Quote: ... the reaction was quenched by addition of the same EDTA solution and the labeled construct was purified using gel-filtration chromatography (Sephadex G-25, PD10 desalting column; GE Healthcare) into 0.9% saline ...