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Citations for GE Life Sciences :
1651 - 1700 of 5304 citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The suspension was then centrifuged for 1 h at 16,000 g at 4 °C and the supernatant was added to 1 mL of glutathione beads (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was cleared by centrifugation at 100,000 x g for 45 min and loaded onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed extensively with NiA buffer ...
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bioRxiv - Genetics 2019Quote: ... centrifuged at 16,000 × g for 30 min and subjected to SDS-PAGE on a SE 250 mini-vertical unit (GE Healthcare). Proteins were transferred to Immobilon-P PVDF membrane (Millipore Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... The most concentrated IR-ECD-containing fraction was either desalted using disposable 8.3 ml Sephadex G-25 PD-10 desalting columns (GE Healthcare) equilibrated with HBS by centrifugation ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The unreacted DBCO molecules were eliminated by a Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences). To make fluorescently labeled DBCO modified FBS proteins or individual proteins (BSA or APO H) ...
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bioRxiv - Developmental Biology 2020Quote: ... After the buffer was replaced with 20 mM sodium phosphate (pH 5.5) buffer containing 25 mM NaCl using a PD MiniTrap G-25 column (GE Healthcare), the digested sample was applied to a HiTrap Q HP column and every 1.5 ml was collected during the application and washing with the same buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were cleared by centrifugation at 100,000 g at 4°C for 30 min and then mixed with Glutathione Sepharose 4B (GE Healthcare). The Sepharose beads were washed with buffer B [50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2019Quote: ... The suspension was centrifuged for 15 minutes at 4°C and 15,000 g to remove cell debris and the clear supernatant was applied to a PD-10 desalting column (GE healthcare) to remove excess free biotin using the gravity protocol according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Labeled NBD1 was spun at 4 °C and 16,000 g for 30 minutes and passed through a gel filtration column (Superdex 200 Increase 10/300 GL - GE Healthcare) previously equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Extracts were cleared by centrifugation for 10 min at 21,000 × g at 4°C and incubated while rotating with 200 μl IgG Sepharose beads (GE Healthcare) for 4 hrs at 4°C ...
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bioRxiv - Physiology 2021Quote: ... The immunostaining was detected using horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) (GE Healthcare, Buckinghamshire, UK; dilution: 1:5000) or anti-mouse IgG (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was clarified by centrifuging at 27,000 x g and the supernatant was applied to a HiTrap-Ni column (GE Healthcare) pre-equilibrated with the lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 33,000 g and loaded on pre-equilibrated Ni-Sepharose 6 Fast Flow (HisTrap FF, GE Healthcare). The column was washed with 20-30 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were spun down at 12,000 g for 30 min and the supernatants were loaded onto a His-Select Nickel Affinity gel (GE Healthcare). Recombinant PstS proteins were eluted with the elution buffer (500 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... DTT was removed via two consecutive passes through a PD-10 Sephadex G-25 M buffer exchange column (GE Healthcare) as per the manufacturer’s instructions into labelling buffer (50 mM HEPES pH 7.1 ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was centrifuged for 10 min at 16,000 × g and the supernatant was incubated with 100 μl (settled volume) of IgG agarose beads (GE Healthcare) at 4°C for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... Clarified cell lysates (following 40 min centrifugation at 11,000 x g and 0.2 μm filtration) were loaded onto prepacked columns (1 ml) of Ni-Sepharose media (His trap, GE Healthcare) using a benchtop peristaltic pump ...
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bioRxiv - Genomics 2021Quote: ... The excess DBCO-NHS was also removed by using Sephadex G-25 desalting columns (GE Healthcare Japan Co., Tokyo, Japan). The resulting DBCO-labeled lectin was then incubated at 4 °C with ten-times the molar amount of 5’-azide-modified DNA oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was clarified by centrifugation at 13,000 x g for 35 min and was passed over Ni-NTA sepharose (GE Healthcare) column ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was cleared by centrifugation at 30,000 g for 30 min and the supernatant was loaded onto a HisTrap FF crude column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Germany) and exchanged to the desired cell culture medium using Sephadex G-25 PD10 desalting columns (GE Healthcare Bio-Science). The final concentration and integrity of eGFP-WNT-3A in the conditioned medium were determined using both ELISA (GFP ELISA® kit ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 39 000 × g for 40 min and the supernatant loaded onto StrepTrap column (GE Healthcare) in lysis buffer containing 50 mM Tris ...
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bioRxiv - Biophysics 2022Quote: ... The solution was subjected to a 5 min spin at 17,000 x g in a refrigerated microcentrifuge before separation on a Superdex 200 10/300 column (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 30,000 g for 30 min at 4°C and incubated for 45 min with amylose beads (GE Healthcare). The loaded protein was cleaved on column by GST-tagged 3C protease at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was collected by centrifugation at 25,000 g for 1 h and incubated with nickel Sepharose resin (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... the sample was clarified 400,000 x g for 30 minutes and applied to a Superdex 200 Increase 10/300 GL (GE Healthcare) gel filtration column equilibrated in G-buffer containing 0.2 M ammonium acetate ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was collected by centrifugation at 25,000 g for 40 min and incubated with nickel Sepharose resin (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700 x g for 12 minutes ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a an isotonic 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700x g for 12 minutes ...
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bioRxiv - Biophysics 2023Quote: ... followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: Elute from HisTrap consisting of the respective protein fractions were centrifuged at 39,000 g for 15 minutes and the supernatant was loaded on a 5 ml StrepTrap HP column (GE Healthcare). The binding buffer used was 150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Probes were end-labelled using [gamma-32P]-ATP and purified using an Illustra ProbeQuant G-50 micro column (GE Healthcare). A mixture of two different probes was used per target ...
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bioRxiv - Cell Biology 2022Quote: ... the lysate was centrifuged at 45,000 g for 30 min and His-(MBP-) ZNF524 further purified on a HisTrapTM HP (GE Healthcare) via the Akta Prime Plus System ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 8 ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 7 ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 15,000 g for 10 min at 20 °C followed by size exclusion chromatography using a PD10 column (GE Healthcare). Importantly ...
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bioRxiv - Immunology 2024Quote: ... Liver infiltrating cells were obtained after 25 min centrifugation (600 g without brake) in a 35% and 67.5% bilayer Percoll (GE Healthcare) gradient ...
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was obtained post the ultracentrifugation at 175,000 g for 1h and loaded onto a HiTrap TALON crude column (GE Healthcare) for affinity enrichment ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were further enriched by centrifugation at room temperature at 695 g for 8 min in 37.5% Percoll (GE Healthcare), prior to washing and resuspension for downstream analysis.
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bioRxiv - Bioengineering 2024Quote: ... The soluble fraction was separated by centrifugation at 30,000×g for 30 min and subjected to size exclusion chromatography using a HiLoad26/60 Superdex75 column (GE Healthcare) with gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer and 0.150 M NaCl) using PD-10 desalting columns or Midi-Trap G-25 (GE Healthcare). The purity of the proteins was evaluated using SDS–PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Molecular Biology 2021Quote: ... 500 µL of 8.1 µM human Notch1fe were loaded on a Superose6 10/300 increase column (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh buffy coats using Ficoll-Paque Plus (GE Healthcare) gradient centrifugation ...
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bioRxiv - Immunology 2022Quote: Human PBMCs were isolated by Ficoll gradient purification as per manufactures protocol (Ficoll, GE-17-1440, GE Healthcare) from human peripheral blood leukapheresis pack (StemCell) ...