Labshake search
Citations for GE Life Sciences :
1601 - 1650 of 4827 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... were immobilized on individual flow cells at 5 μl/min using a streptavidin-coated sensor chip (Series S Sensor chip SA, GE Healthcare). Three-fold serial dilutions of recombinant DLL4 proteins (N-EGF5 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Eluted protein was diluted to a final salt concentration of 200 mM NaCl and loaded onto a 5 mL HiTrap SP ion-exchange column (GE Healthcare).The protein was eluted in the same buffer with a salt gradient (200mM-1 M NaCl ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1mM DTT) to the 50 mM NaCl final concentration and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer B ...
-
bioRxiv - Microbiology 2020Quote: ... the VHH-72-Fc protein in the cleared cell culture medium was captured on a 5 mL MabSelect SuRe column (GE Healthcare), eluted with a McIlvaine buffer pH 3 ...
-
bioRxiv - Biochemistry 2021Quote: ... The sample was diluted 5-fold with buffer A (50 mM Tris pH 7.5, 10 mM β-mercaptoethanol) before loading onto a MonoS 5/50 GL column (GE Healthcare) with buffer A supplemented with 100 mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... The sample was diluted 3-fold with dilution buffer (50 mM Tris pH 7.5, 10 mM β-mercaptoethanol) before loading onto a MonoS 5/50 GL column (GE Healthcare) with buffer A (50 mM Tris pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: ... and soluble supernatant was purified using HisTrap FF 5 mL immobilized metal ion affinity chromatography columns (GE Healthcare, New Jersey, USA) and eluted with 250 mM imidazole ...
-
bioRxiv - Biochemistry 2021Quote: ... The buffer salt concentration was diluted to 30 mM NaCl and the solution was flown through a 5 mL HiTrap Q HP (GE Healthcare) and a NiNTA column ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was then diluted 10 times with mono Q binding buffer (50 mM HEPES, pH 8.0) and applied to a MonoQ 5/50 GL column (GE Life Sciences) connected to an AKTA Pure system (GE Life Sciences). ...
-
bioRxiv - Biophysics 2020Quote: ... The flow-through that contained the protein of interest was then applied to a HiTrap Q 5 mL column (GE Healthcare). The Q column flow-through containing nsp8 or nsp7 was concentrated using a MWCO 3 kDa Amicon Ultra Centrifugal Filter (Merck ...
-
bioRxiv - Biophysics 2021Quote: ... The supernatant containing the solubilized proteins was supplemented with 40 μg/ml avidin and subsequently loaded onto a 5-ml StrepTrap HP column (GE Healthcare), which was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
-
bioRxiv - Microbiology 2022Quote: ... Cleared lysates containing proteins with no His tag were purified by cation exchange chromatography (CIEX) using a 5 ml HiTrap Sepharose SP FF column (GE Healthcare) fitted on an ÄKTA fast protein liquid chromatography (FPLC ...
-
bioRxiv - Cell Biology 2020Quote: ... Buffer-exchange into 40 mM Tris-Cl pH 7.0 was carried out using a 5 ml HiTrap™ desalting column (GE Healthcare) as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Images were acquired from 15 fields at 5- or 10-minute intervals using a DeltaVision Elite Deconvolution Microscope (GE Healthcare Biosciences) with a 60x oil immersion objective and a Front Illuminated sCMOS camera ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
-
bioRxiv - Developmental Biology 2022Quote: ... The media was buffer exchanged with PBS and His-tagged proteins were captured with 5 mL HisTrap Excel columns (GE Healthcare), washed with 20 mM imidazole and eluted with 300 mM imidazole containing PBS buffer ...
-
bioRxiv - Microbiology 2022Quote: ... it was passed through a 0.22 µm filter to remove potential precipitates and applied onto a 5 ml HisTrap HP column (GE Healthcare, USA) equilibrated with buffer BZ3 ...
-
bioRxiv - Biophysics 2019Quote: ... Cell lysate was centrifuged at 35,000 g for 60 minutes to remove insoluble cell debris and the soluble lysate was loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
-
bioRxiv - Biophysics 2019Quote: ... The protein was further purified using reverse IMAC with the cleaved protein being loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
-
bioRxiv - Biophysics 2019Quote: ... All fractions identified to contain the cleaved protein were concentrated to a volume of 5 mL and loaded onto a HiLoad 16/60 Superdex 75 gel filtration column (GE Healthcare) that had been pre-equilibrated with either crystallography buffer (20 mM Bis-Tris pH 7.0 ...
-
bioRxiv - Immunology 2019Quote: ... Membranes were blocked with 5% bovine serum albumin and incubated with the appropriate primary and HRP-conjugated secondary antibodies (GE Healthcare). Western Lightning ECL Pro (PerkinElmer ...
-
bioRxiv - Biochemistry 2019Quote: ... the dialyzed protein was filtered and loaded on a cation exchange HiTrap SP HP 5 ml column (GE healthcare life sciences) pre-equilibrated with 5 column volumes of buffer ASP (100 mM Ammonium acetate ...
-
bioRxiv - Cell Biology 2019Quote: ... Southern analysis was performed by separating DNA fragments in 1% agarose/1X TBE gels using pulse-field electrophoresis with a 5-45 s ramp and blotted onto Hybond-XL membranes (GE Healthcare) with alkaline transfer.
-
bioRxiv - Molecular Biology 2019Quote: α-Syn was further purified with Ni-NTA affinity chromatography using a HisTrap HP 5 mL column (GE healthcare life sciences, USA) connected to an ÄKTA system (GE healthcare life sciences ...
-
bioRxiv - Biochemistry 2020Quote: The oligomeric state of proteins and interaction between 14-3-3 and STARD1 (or STARD3) constructs were analyzed by size-exclusion chromatography on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at 0.1 ml/min flow rate using a Varian ProStar 335/ProStar 363 system ...
-
bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
-
bioRxiv - Biochemistry 2020Quote: ... Eluate from GSH affinity chromatography was subjected to heparin affinity chromatography using a HiTrap HP Heparin column (5 mL GE Healthcare) equilibrated with NMR buffer (10 mM MES ...
-
bioRxiv - Cancer Biology 2020Quote: Secreted form of the PD-L1 carrying C-terminal 10x His tag was purified using AKTA Start FPLC and Tricorn 5/100 column (both GE Healthcare) filled with HisPur Ni-NTA Resin (Thermo # 88223 ...
-
bioRxiv - Biochemistry 2020Quote: ... The elute was concentrated to 5 ml and applied to a gel filtration run on a 16-600 HiLoad Superdex 200 pg column (GE Healthcare), preequilibrated with 20 mM Hepes (pH 7.0) ...
-
bioRxiv - Microbiology 2020Quote: ... and the soluble His6-M protein was purified on an Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
-
bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with 5 μl rabbit polyclonal anti-GM130 serum or 5 μl pre-immune serum for 60 min at 4°C and incubated with protein A-Sepharose (GE Healthcare) for additional 60 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... of the purified hPIV3 L-P complex was determined by analytical size-exclusion chromatography using a Superdex 200 Increase 5/150 GL column (GE healthcare) with buffer B ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... After concentration by spin filtration to 4-5 mL pro-rhodesain was loaded onto a HiLoad 16/600 Superdex 75 pg column (GE Healthcare), and purified by SEC (0.5 mL/min ...
-
bioRxiv - Immunology 2019Quote: ... SI-LP and LI-LP cells were purified by density gradient centrifugation (600 rcf for 20 min at 22 °C, acceleration 5 and brake 0) with 40/70 % Percoll (GE Healthcare). Cell suspensions were subsequently filtered through 100 µm cell strainers (BD Biosciences ...
-
bioRxiv - Biochemistry 2019Quote: ... A 50 – 500 mM KCl gradient was used to elute IF2 from a 5 ml Hi-Trap SP HP column (17115201, GE Healthcare). Fractions were analysed by 8% acrylamide SDS-PAGE and those containing IF2 were pooled together ...
-
bioRxiv - Neuroscience 2019Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at - 80°C until use ...
-
bioRxiv - Biochemistry 2019Quote: ... All Rab proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Non-specifically bound proteins were removed by a step wash with buffer B (50 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: ... Diafiltrated cell supernatant was purified by immobilized nickel affinity chromatography (5-ml fast flow crude column and ÄKTA fast protein liquid chromatography [FPLC] system; GE Healthcare) at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... coli and purified using our previously reported protocols.17 TNKS2 constructs were cloned into pNIC-MBP expression vectors and an additional purification on an MBPTrap HP 5 ml column (GE Healthcare) prior to cleavage with TEV protease was performed.20 Details of the constructs for each PARP enzyme are listed in Table S1.
-
bioRxiv - Cell Biology 2022Quote: ... 5 min at 4 °C followed by an anion exchange chromatography loading the supernatant on a HiTrap Q HP 5 ml column (GE Healthcare) and intensive wash step of 20 column volumes with ion exchange buffer A ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT in the presence of TEV protease and subsequently purified using a 5 mL HiTrap HP Heparin column (GE Healthcare), eluting with a buffer containing 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2021Quote: Purified SMO pCT was concentrated to 5 mg/ml and analyzed via SEC-MALS using a Superdex 75 gel chromatography column (GE Healthcare) equilibrated in gel filtration buffer (50 mM HEPES pH 7.5 ...
-
bioRxiv - Biophysics 2020Quote: ... Uncleaved protein and cleaved hexahistidine tag were removed from the eluted mixture with a HisTrap HP 5 mL column (GE Healthcare) in PBS with 25 mM imidazole ...
-
bioRxiv - Immunology 2020Quote: ... Samples containing activity were fractionated by anion exchange chromatography on a Mono Q 5/200 GL column (GE Healthcare, Piscataway NJ) using a gradient from 50mM Tris-HCl (pH 7.4 ...
-
bioRxiv - Microbiology 2020Quote: ... The cell debris was pelleted by centrifugation and the supernatant was loaded onto a 5 ml HisTrap IMAC HP Nickel Sepharose column (GE Healthcare), using an ÄKTA Pure chromatography system (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were precipitated the supernatant by addition of ammonium sulfate to 67% saturation and dissolved in buffer D and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer D ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 13,000 g for 60 minutes and the filtered supernatant was loaded onto a 5 mL Ni-NTA column (GE Healthcare, USA) equilibrated with binding buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2021Quote: ... FLAG-eluted motor domains (wild-type + jordan) were bound to a strong anion exchanger (5/50 MonoQ GL; Cytiva) using a Purifier 10 chromatography system (GE Healthcare). The column was washed with MOPS (10) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cleaved MBP tag and TEV protease were removed by anion exchange chromatography using a Mono Q 5/50GL (GE Healthcare) column pre-equilibrated with buffer-B ...