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Citations for GE Life Sciences :
1501 - 1550 of 4827 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Elute from HisTrap consisting of the respective protein fractions were centrifuged at 39,000 g for 15 minutes and the supernatant was loaded on a 5 ml StrepTrap HP column (GE Healthcare). The binding buffer used was 150 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was diluted 5-fold to contain 100 mM NaCl and purified over SP-Sepharose (HiPrep SPFF, GE Healthcare) using a linear gradient of buffer containing 2 M NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 and equilibrated with wash buffer (50 mM K-HEPES pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biophysics 2023Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using CL-Xposure films (Cat.no ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 ...
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) and further purified via size exclusion chromatography (SEC) in an S75 5/150 GL increase column (GE Healthcare). Protein collected from SEC was normalized to 1 μM where possible.
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples were subjected to electrophoresis through 5% native polyacrylamide gel in 0.5x TBE buffer and imaged with 9410 Typhoon variable mode imager (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... the KhpA and KhpB-ΔN were secondly purified by cation-exchange chromatography (HiTrap SP 5 ml, GE Healthcare Life Science) followed by SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using the Chemidoc XRS imaging system (Bio-Rad)..
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were developed using 1:1 Amersham ECL western blotting reagent (GE Healthcare) to SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Capto Core 700 beads (GE Healthcare; ~1:1 resin volume to sample volume) were used to remove residual dyes and enzymes from the solution of labeled virus ...
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bioRxiv - Biochemistry 2021Quote: ... a 1:1 mixture of 50 mg/mL Sera-Mag SpeedBeads (GE Healthcare) and 50 mg/mL Sera-Mag SpeedBeads (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-FBH1[1-250] in the pGEX6P-1 vector (Amersham/GE Healthcare/Cytiva) was introduced into BL21:DE3 Rosetta cells (Novagen) ...
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bioRxiv - Physiology 2019Quote: ... 20 islets per well (n = 3 biological replicates per group) were transfected with control (non-targeting #2) siRNA or mouse Brd4 SMARTpool siRNA (Dharmacon GE Life Sciences) at 100 nM each with lipofectamine 3000 according to the product instructions ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Molecular Biology 2023Quote: ... The signals of the transferred radioactively labelled methyl groups were detected by autoradiography using a HyperfilmTM high performance autoradiography film (GE Healthcare) at −80LJ°C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Microbiology 2019Quote: ... Blots were UV cross-linked and baked at 80°C for 2 h prior to pre-hybridization at 42°C for 1 h in Rabid-Hyb Buffer (GE Healthcare Life Sciences). Hybridization with α-32P-ATP end-labeled DNA oligo probes was performed at 42°C for 2-16 h ...
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bioRxiv - Neuroscience 2022Quote: ... installed prone on the MRI bed (Fig 1) and anaesthesia was immediately switched to 1.5-2% isoflurane (28) in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... and size-exclusion (Superose 6; GE Healthcare) chromatography ...
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bioRxiv - Immunology 2020Quote: ... and Superose 6 (GE Healthcare Life Sciences) size exclusion chromotography (SEC) ...
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bioRxiv - Immunology 2020Quote: ... and a Superose 6 column (GE Healthcare). Before sample loading ...
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bioRxiv - Biophysics 2023Quote: ... Sepharose 6 Fast Flow beads (GE Healthcare) were packed in a column (packed volume 10 L ...
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bioRxiv - Biochemistry 2022Quote: ... Superose 6 increase 10/300 (GE Healthcare) was utilized for analysis of gephyrin and LS variants complexes ...
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bioRxiv - Bioengineering 2023Quote: ... and AKTA Flux 6 (GE Healthcare, USA) were used for buffer exchange in this unit process step ...
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bioRxiv - Biophysics 2023Quote: ... and size-exclusion (Superose-6; GE Healthcare) chromatography.
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant was filtered using a 0.45 µm filter and then loaded onto a 5 ml HisTrap™ HP nickel sepharose column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and loaded to HisTrap FF crude 5 ml Ni-NTA column (GE healthcare, #17-5286-01). Column was washed with 10 column volume of lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Microbiology 2019Quote: ... experiments coupled to multi-angle laser light scattering (MALS) and refractometry (RI) were performed on a Superdex S200 5/150 GL increase column (GE Healthcare). 25 μl of AdhE mutant protein were injected at a concentration of 10 mg.ml−1 in buffer 0.05M Tris HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 or 10 µg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the dialyzed sample was loaded on an anion exchange HiTrap Q HP 5 mL column (GE healthcare life sciences, Boston, Massachusetts) pre-equilibrated with 20 mM bis-Tris pH 5.5 ...
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bioRxiv - Biophysics 2022Quote: All samples were pre-reduced with 5 mM DTT for one hour followed by desalting using a PD10 column (GE Healthcare) into 20 mM tris pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... Cell disruption was achieved by sonication (Branson Sonifier SFX250) and the soluble fraction was subjected to immobilized metal affinity purification using a 5 ml Ni-NTA column (GE healthcare) connected to a FPLC system (GE healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... The eluted fractions were concentrated to 5 ml and loaded onto a HiLoad 26/600 Superdex 200 prep grade (GE Healthcare) equilibrated with the SEC buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Cell Biology 2019Quote: Cells were fixed with 4% paraformaldehyde for 5 min and images were captured at room temperature on a DeltaVision fluorescence microscope (Applied Precision) installed with softWoRx version 5.5 housed in the Micron Oxford essentially as described [13] ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate was clarified by centrifugation at 35,000g and purified by immobilized metal affinity chromatography (IMAC) on a 5 mL Fast Flow Chelating Sepharose column (GE Life Sciences) with an elution gradient from 0 to 200 mM imidazole over 120 mL ...
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bioRxiv - Neuroscience 2019Quote: ... Labeled proteins were separated from the free fluorophore using a NAP-5 Sephadex G-25 desalting columns in labeling buffer (GE Healthcare). The labeling efficiency was determined using an extinction coefficient of 5400 M-1cm-1 at 335 nm for IAEDANS and the protein concentration was determined by Bradford assay using BSA as a standard ...