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Citations for GE Life Sciences :
1551 - 1600 of 4827 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 1.5 μM PCNA trimer and 20 μM TTP was loaded onto a Superdex 200 increase 3.2/300 column (GE Life Sciences) equilibrated with a buffer comprising [25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2019Quote: ... as described previously for the non-fluorescent version (39): i) affinity purification of filtered supernatant with 5 ml HisTrap FF column (GE Healthcare) and step elution with 300 mM imidazole ...
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bioRxiv - Biophysics 2019Quote: ... The protein solution was then syringe filtered using a 0.22 µm PES syringe filter and 5 mL were injected onto a Highload Superdex200 16/600 size exclusion column (GE Healthcare). During size exclusion chromatography ...
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bioRxiv - Biophysics 2019Quote: ... Rab8-His6 proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Unspecifically bound proteins were removed by a step wash with 5% buffer B (50 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... The protein solution was then syringe filtered using a 0.22 μm PES syringe filter and 5 mL were injected onto a Highload Superdex200 16/600 size exclusion column (GE Healthcare). During size exclusion chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was heat-inactivated for 5 min at 95°C and the free nucleotides were removed on a Sephadex G-25 column (GE Healthcare). The oligo was annealed to a 1.5x excess of the unlabelled complementary strand by heating for 5 min to 80°C and slowly cooling to RT in annealing buffer (50 mM Hepes ...
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bioRxiv - Bioengineering 2020Quote: ... The homogenate was centrifuged as before and the supernatant was purified on an AKTA Pure FPLC equipped with a HiTrap Q 5 mL anion exchange column (GE Healthcare). Solvent A was 20 mM tris pH 8.5 ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was purified using an AKTA Pure FPLC equipped with a HisTrap FF crude 5 mL column (GE Healthcare). Solvent A was 20 mM tris pH 7.5 ...
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bioRxiv - Bioengineering 2019Quote: Cell-free supernatants were prepared by centrifugation and directly loaded (100 mL) onto a 5 mL HiTrap MabSelect column (GE Healthcare). After washing the column with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Cancer Biology 2019Quote: ... All fractions identified to contain cleaved RNF114 were concentrated to a volume of 5 mL and loaded onto a HiLoad 16/60 Superdex 75 gel filtration column (GE Healthcare) that had been pre-equilibrated with either crystallography buffer ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was stopped on ice and labeled polyP300-AF647 was separated from free dye and unlabeled polyP via a NAP-5 column (GE Healthcare) that was equilibrated with 40 mM KPi ...
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bioRxiv - Immunology 2019Quote: ... the cell suspension diluted in 1 mL of complete L15 medium was carefully layered on top of 5 mL of Percoll gradient (L15/10 mM HEPES/20% Percoll (GE Healthcare) and centrifuged at 400 g for 9 min ...
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bioRxiv - Genomics 2019Quote: ... cell lysates were treated with 5 units RNase I (ART-Seq) per OD260 and monosomes were purified in MicroSpin S-400 columns (GE Healthcare). For both RPF- and RNA-seq ...
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bioRxiv - Biochemistry 2020Quote: ... Tag and TEV protease removal was achieved by re-loading the dialyzed sample onto the 5 mL HisTrap Excel column (GE Healthcare), and collecting the unbound tagless sample ...
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bioRxiv - Biochemistry 2020Quote: ... The protein of interest (NT-mini or NT*-mini) was then eluted into a 5 ml Ni-IMAC HisTrap Excel column (GE Healthcare) by further increasing the NaCl concentration to 600 mM (buffer NT.B) ...
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bioRxiv - Neuroscience 2020Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at -80°C until use ...
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bioRxiv - Biophysics 2020Quote: ... centrifuged at 14000g and 4 °C for 5 min and the excess of ammonium sulfate was removed by running the supernatant through a NAP-25 column (GE Healthcare). The tAv was purified using HiTrap chelating column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: Fifty milliliters of the supernatant was loaded onto a 50 mL superloop (Amersham Biosciences) and bound to a 5 mL heparin column (HiTrap Heparin HP 1X5 mL, GE Healthcare) by fast protein liquid chromatography (ÄKTApurifier ...
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bioRxiv - Biochemistry 2020Quote: ... The cleaved protein was concentrated to 5 ml and loaded in a HiLoad 16/600 Superdex 75pg gel-filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Cleaved protein was passed through a 5 ml HisTrapFF column and further purified by size-exclusion chromatography (S200 16/60 column; GE Healthcare) in buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Soluble RNA was separated from solid acrylamide by filtration through 0.22 micron filters and then manually applied to a 5 mL HiTrapQ column (GE Healthcare # 29051325) that had been equilibrated in buffer D (300 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were clarified by centrifugation (20 minutes at 18,000x g) and bound to a 5 mL HisTrap™ FF Crude column (GE Healthcare) and eluted with 50-75 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... 4°C for 30 minutes and loaded onto a 5 ml HighTrap MBP column (GE Healtcare) using an Äkta-Pure system (GE Healthcare). The column was washed with lysis buffer and protein eluted with 10 column volumes MBP-elution buffer (100 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... The dialyzed media was filtered again and 5 mM imidazole was added to the media before loading onto a Histrap column (GE healthcare). The protein was eluted using a linear imidazole gradient (20 to 500mM ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation (40000 g for 30 min) and the supernatant applied onto a 5 ml StrepTrap column (GE Healthcare). After washing with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: The oligomeric state of proteins as well as protein-protein and protein-tRNA interactions were analyzed by loading 50 μl samples on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate using a Varian ProStar 335 system (Varian Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... 10.2 µM protein was incubated with 100 µM probe and 0.5 mM TCEP for ∼20 h at 4 °C and free probe was removed by desalting using a 5 mL HiTrap desalting column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... 0.1 mM EDTA) before further purification by ion-exchange on a MonoQ 5/50 Gl column (GE Healthcare Pte Ltd Singapore) to remove traces of vector and recombination products ...
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bioRxiv - Immunology 2020Quote: ... cells were fed with 5% of the starting volume with Cell Boost7a and 0.5% Cell Boost7b (Hyclone GE Healthcare Life Sciences). Cell counts and glucose was measured as previously described,70 On day 8 ...
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bioRxiv - Neuroscience 2019Quote: ... the protein was eluted using 400 mM Imidazole (5 CV) and loaded onto a HiLoad 16/600 Superdex 200 pg size exclusion column (GE Healthcare). The main peak was collected ...
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bioRxiv - Neuroscience 2019Quote: ... The mixture was then purified via reverse Ni-NTA and the buffer was changed to NMR buffer (see above) using either a NAP-5 desalting column (GE Healthcare) or by S200a size exclusion chromatography.
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bioRxiv - Physiology 2021Quote: ... guinea pigs were anesthetized (4-5% isoflurane with 2L/min O2) and placed on a micro-PET scanner (GE eXplore Vista, GE Healthcare) fitted with a nose cone to inhale 2.5-3% isoflurane with 1L/min O2 for maintenance of anesthesia ...
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bioRxiv - Molecular Biology 2020Quote: ... divided into aliquots of 10 mL and each aliquot was added on top of a 5 mL of Ficoll-Paque Premium (GE Healthcare) in 15 mL sterile Falcon tubes ...
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bioRxiv - Microbiology 2020Quote: ... VHH containing fractions were pooled and concentrated and the elution buffer was exchanged with PBS with a Vivaspin column (5 kDa cutoff, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture containing the protein–DNA complex were resolved on a 5% native PAGE gel on ice and transferred to a nylon membrane (Amersham Hybond-XL, GE healthcare) using 0.5 X TBE ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T (ATCC CRL-3216) cell lines used in this study were cultured at 37°C in 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was then centrifuged at 37,500 g for 30 min and the supernatant incubated with 5 mL GFP nanobody-coupled CNBr-activated Sepharose resin (GE Healthcare) for 2 hrs (Kubala et al. ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl and 10 mM β-ME and loaded onto a 5 ml HiTrap SP anion exchange column (GE Healthcare). The binary complex ScNatC was then eluted using a salt gradient (50–750 mM NaCl) ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
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bioRxiv - Biochemistry 2022Quote: ... of GlOCPX or SynOCP1 samples (5 mg/ml in 50 μl) was performed on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a Prostar 335 detector (Varian ...
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bioRxiv - Biochemistry 2022Quote: ... Individual reactions were transferred to a 96-well plate and analysed using a Superdex 200 Increase 5/150 GL gel filtration column (GE Healthcare) attached to an Agilent 1200 Series Gradient HPLC system ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... immobilized protein was eluted using wash buffer and further loaded onto an ion-exchange column (MonoQ 5/50 GL column, GE Healthcare) to achieve highly pure PBAF complex ...
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bioRxiv - Neuroscience 2022Quote: ... TE = 20 ms) were acquired both before and 5 min after intravenous injection of 100µl gadolinium-DTPA (Omniscan®, GE Healthcare). The matrix size and field of view were 256 x 256 and 3.5 cm x 3.5 cm ...
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bioRxiv - Microbiology 2022Quote: ... The protein was then concentrated to a final volume of 500 μl using Vivaspin 20 with a molecular weight cut off (MWCO) of 5 kDa (GE Healthcare). The sample was loaded on a Superose6 Increase 10/300 GL size exclusion column from GE Healthcare that had been equilibrated with size exclusion buffer (for INpro-28R ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Insoluble material was pelleted at 50,000 xg for 30 min at 4°C and the supernatant loaded directly onto a 5 ml His Trap HP (GE Healthcare) at 2 ml.min-1 and washed with 50 ml Buffer A at 4 m.min-1 at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Reaction mixtures were separated on a 5% non-denaturing polyacrylamide gel in TGE buffer (25 mM Tris, 25 mM Glycine and 5 mM EDTA) and analyzed using a Typhoon trio imager (GE Healthcare). For the short–Long EMSA Assay Coupled to Differential Fluorescent DNA Labeling ...
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bioRxiv - Biophysics 2022Quote: ... The solution was passed through a 0.22 μm membrane filter and loaded onto a 5 ml HiTrap Q HP anion exchange column (GE Healthcare, USA) equilibrated with wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT in the presence of TEV protease and subsequently purified using a 5 mL HiTrap HP Heparin column (GE Healthcare), eluting with a buffer containing 20 mM Tris-HCl pH 8.0 ...