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Citations for GE Life Sciences :
101 - 150 of 948 citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The soluble fraction of the lysate was loaded on a nickel column (HisTrap Hp, GE healthcare). The column was extensively washed with wash buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant containing soluble proteins were collected and allowed to flow through GST4B resin (GE Healthcare). After washing with three column volumes of sonification buffer ...
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bioRxiv - Microbiology 2019Quote: ... The soluble His-tagged protein was purified using a HisTrap column (GE Healthcare Life Sciences, USA). Purity of the eluted protein was checked by SDS-PAGE gel staining with Coomassie Blue ...
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bioRxiv - Microbiology 2022Quote: ... The soluble fraction was loaded onto a Ni Sepharose™ 6 Fast Flow agarose (GE Healthcare) column for protein purification ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was purified by affinity chromatography using an ÄKTA Explorer FPLC system (GE Healthcare) loaded with a Nickel Sepharose FF 8 mL resin column ...
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bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble extracts were loaded onto a Q-Sepharose Fast-Flow column (65 mL, GE Healthcare) previously equilibrated with 20 mM Tris- HCl buffer pH 7.5 containing 18 % glycerol (buffer A) ...
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bioRxiv - Biochemistry 2024Quote: ... the soluble fraction was incubated in batch for 2 h with glutathione Sepharose 4B (GE Healthcare), previously equilibrated in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble cell lysates were applied on a Ni-sepharose excel (GE Healthcare, #17-3712-01), packed in a column of 4 ml bed volume with Sl1 buffer buffer (20mM Tris ...
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bioRxiv - Biophysics 2019Quote: ... PreScission protease was removed by binding to GST-Sepharose (GE Healthcare) for 2 h and buffer exchange with centrifugal filter devices (10,000 MWCO ...
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bioRxiv - Microbiology 2020Quote: Binding affinities were measured using a MicroCal iTC200 system (GE Healthcare) at 25°C using wild type TANV16L as well as two mutants TANV16L K52A and R90A in 25 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2023Quote: Direct binding experiments were performed on a Biacore 4000 (GE Healthcare) in 10 mM HEPES (pH 7.4) ...
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bioRxiv - Immunology 2023Quote: ... supernatants containing expressed trimers were placed in an agarose-bound Gallanthus Nivalis Lectin (GNL) or CNBr-activated Sepharose 4B bead (GE Healthcare) bound PGT145 bnAb antibody affinity columns for purification ...
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bioRxiv - Microbiology 2023Quote: ... Gp140 proteins were purified from clarified cell culture supernatants 4 days post-transfection using Galanthus nivalis lectin (GNL) affinity chromatography followed by a Q-sepharose (GE Healthcare) polishing step to remove host cell protein contaminants ...
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bioRxiv - Cell Biology 2019Quote: ... The tiny fraction of soluble fusion was purified using glutathione sepharose following the manufacturer’s instructions (GE Healthcare). Polyclonal antibodies were generated using injections of 0.5 – 1mg protein (R and R Research Co.) ...
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bioRxiv - Microbiology 2019Quote: ... The protein was purified from the soluble fraction using HisTrap HP and Superdex 75 columns (GE Healthcare) in 50 mM HEPES (pH 7.4) ...
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bioRxiv - Immunology 2022Quote: ... The soluble fraction was then allowed to bind to Ni-NTA resin (GE Healthcare Life Sciences, Amersham) at 25 °C and eluted in a gradient of imidazole (100 mM ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant containing soluble proteins was collected and allowed to flow through Ni-NTA resin (GE Healthcare). After washing with three column volumes of sonification buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble supernatants were purified by nickel-affinity chromatography (HiTrap Chelating HP column, GE Healthcare Life Science) using an NGC Quest 10 Plus Chromatography System (BIO-RAD) ...
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bioRxiv - Genetics 2020Quote: ... The soluble fraction was subjected to an affinity purification using a MBP FF crude column (GE Healthcare) for the KNL-2 fusion construct ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was applied to a 5 mL HisTrap HP Ni2+-NTA IMAC column (GE Healthcare) pre-equilibrated with lysis buffer and washed with 50 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was purified via immobilized metal affinity chromatography (IMAC) on a HisTrapTMFF column (GE Healthcare) and eluted with a linear gradient to 40 mM HEPES pH 7.1 ...
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bioRxiv - Immunology 2020Quote: ... The soluble fraction was purified from the cell lysate using Glutathione Sepharose 4B beads (GS4B, GE Healthcare). The proteins were then digested with PreScission protease (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Soluble supernatants were purified by affinity chromatography (StepTag or HiTrap Chelating HP columns, GE Healthcare Life Science) using an NGC Quest 10 Plus Chromatography System (BIO-RAD ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified from soluble fractions of the bacterial cell lysates using Glutathione Sepharose beads (GE Healthcare). Proteins were dialyzed in 1x TBS overnight at 4°C overnight and protein quantified ...
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bioRxiv - Cell Biology 2019Quote: ... was purified by Maltose Binding Protein Trap (MBPTrap)-affinity column (GE Healthcare) and eluted in 20mM Tris ...
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bioRxiv - Cell Biology 2021Quote: SPR binding experiments were collected on a Biacore T200 Instrument (GE Healthcare). Anti-His6 monoclonal antibody (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... Heats of binding were measured using a MicroCal iTC200 calorimeter (GE Healthcare) with a stirring rate of 1000 rpm at 27 °C ...
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bioRxiv - Molecular Biology 2019Quote: All binding measurements were conducted using a BIAcore T100 instrument (GE Healthcare). Biotinylated RNF43 ...
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bioRxiv - Genetics 2021Quote: We performed SPR binding analysis using a Biacore T200 instrument (GE Healthcare) at room temperature in HBS-EP(+ ...
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bioRxiv - Immunology 2021Quote: SPR binding studies were performed using a Biacore T200 (GE life sciences). All studies were performed at 25°C in running buffer consisting of 10mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody binding was detected by enhanced chemiluminescence (GE Healthcare; Buckinghamshire, United Kingdom). Protein quantification was performed using a GS-900 Calibrated Densitometer (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... binding curves were analyzed using BIAevaluation software (GE Healthcare, Marlborough, MA, USA) following standard procedures ...
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bioRxiv - Pathology 2024Quote: ... Lysates were purified through binding to Fast flow Nickel sepharose (GE Healthcare) and washed with buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were then purified via binding to glutathione Sepharose (GE Healthcare) and washed ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Cell Biology 2021Quote: ... The soluble fraction was then injected onto a Superdex 200 Increase 10/300 GL (GE Healthcare Bio-Sciences) size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble fraction was subjected to an affinity purification using a chelating HiTrap FF crude column (GE Healthcare) charged with Ni2+ ions on an AKTA-HPLC purifier (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was added to a 5 mL Ni-Sepharose fast-flow resin (GE Healthcare Life Sciences) pre-equilibrated with buffer A at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Soluble aliquots were pooled and loaded onto a Superdex 200 GL 10/300 column (GE Healthcare, Chicago, USA) in a protein buffer (20 mM TRIS-HCl□pH 7.4 and 150□mM NaCl).
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bioRxiv - Cell Biology 2023Quote: ... The VacA-containing soluble fraction was loaded onto an anion exchange column (DEAE Sephacel, GE healthcare, Chicago, IL) pre-equilibrated with wash buffer ...
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bioRxiv - Genetics 2023Quote: ... for 45 min and soluble extract was applied to a 5 ml HisTrap FF column (GE healthcare Inc.) using a GE AKTA Avant 25 FPLC system ...
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction was loaded onto a 1-mL anion exchange column (HiTrap 1 mL HP, GE Healthcare) and purified on an Akta purifier system ...
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bioRxiv - Plant Biology 2023Quote: ... The soluble fraction was loaded on a Ni2+ affinity column (HisTrapTM Chelating HP Column, GE Healthcare, Uppsala, Sweden) using a peristaltic pump with a flow rate of 1 mL min-1 ...
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bioRxiv - Biophysics 2024Quote: ... The soluble fraction of gp24 was incubated with glutathione sorbent slurry (Glutathione Sepharose 4B; GE Healthcare Life Sciences), washed with phosphate buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The soluble homogenate cleared by centrifugation was loaded onto Talon resin (Clonetech) followed by glutathione Sepharose (GE Healthcare) to obtain the correct stoichiometry ratio of the Retromer complex ...
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bioRxiv - Immunology 2019Quote: Binding to FcγRs was carried out using a Biacore T200 biosensor (GE Healthcare). Recombinantly expressed FcγRS (R&D systems or Sino Biologicals ...
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bioRxiv - Biochemistry 2020Quote: Measurements of heparin binding were performed in a Biacore T200 instrument (GE Healthcare) using a heparin-coated chip (Xantec) ...
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bioRxiv - Biochemistry 2020Quote: ... which allows affinity purification by binding to a StrepTactin Sepharose resin (GE Healthcare). Cells were grown in LB medium at 37°C with agitation to OD600nm = 0.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (GE Healthcare).