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Citations for GE Life Sciences :
401 - 450 of 948 citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Background binding to blank immobilized flow cells was subtracted and affinity constants were calculated using BIA Evaluation software (GE Healthcare) using the Steady State Affinity model ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing tagged-SbtB7001 were pooled and transferred into binding buffer using a HiPrep 26/10 Desalting column (GE Healthcare). The His6–Ub tags were removed using a ubiquitin carboxyl-terminal hydrolase 2 catalytic core domain (USP2cc):SbtB7001 molar ratio of 1:25 at 4 °C overnight as previously described [14] ...
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bioRxiv - Microbiology 2020Quote: ... and the kinetic parameters were obtained by globally fitting the double-reference subtracted data to a 1:1 binding model with mass transport limitation using Biacore T200 Evaluation software v 3.1 (GE Healthcare). The dissociation rate constant (kd ...
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bioRxiv - Biophysics 2020Quote: The binding affinities of the Fab antibody fragment Fab705 for full-length MgtE were measured using MicroCal iTC200 (GE Healthcare). The full-length MgtE proteins were purified by a similar procedure to that described above but with SEC buffer [buffer B containing 0.05% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... This was followed by performing SEC using GST binding buffer supplemented with 0.5 mM TCEP on a Superdex 200 10/300 column using an ÄKTA purifier (GE Healthcare). The fractions corresponding to the peak were pooled ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
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bioRxiv - Biochemistry 2022Quote: ... fits of the sensorgrams were determined using a 1:1 binding model (Langmuir) using Biacore T200 Evaluation Software 3.1 (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... Flow cell 1 was left empty to use this flow cell as a reference flow-cell for on-line subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface using Biacore T100 Control Software (version 3.2) (GE Healthcare). FPLC-purified non-biotinylated protein was used as analyte ...
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bioRxiv - Immunology 2022Quote: ... and the raw data curves were fitted to a 1:1 binding model using Biacore 8K Evaluation Software (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... and the binding affinities were obtained using a 1:1 (Langmuir) binding fit model with Biacore T200 Evaluation software version 3.2 (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: The binding affinity of b96.11 to apo- and holo- GAD65 was measured using surface plasmon resonance (BIAcore T-100, GE Healthcare). Purified monoclonal b96.11 IgG antibody was captured on a CM5 sensor chip with immobilised Protein G ...
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bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Overexpressed recombinant proteins in supernatants were purified by AKTA start affinity chromatography system HiTrap Mabselect (GE Healthcare) according to the manufacturer’s instructions (S1 Fig).
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bioRxiv - Biochemistry 2019Quote: ... The recombinant Mtb-MenD protein was purified by IMAC with 5 mL HisTrap HP columns (GE Healthcare) using an imidazole gradient of 20–500 mM over 90 mL ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 recombinant proteins were purified over a His-TRAP FF column (GE Healthcare) with 1-mL bed volume ...
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bioRxiv - Microbiology 2021Quote: ... recombinant protein was purified from the filtered culture supernatant using HisTrap Excel columns (GE Healthcare Life Sciences). Each column was stored in 20% ethanol and washed with 5 column volumes (CV ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant 3CLpro were then purified using HIS Gravitrap Ni-NTA affinity columns (GE Healthcare, Chicago, IL), following the standard protocol (Kim et al. ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant WT and mutant IN molecules were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... The GST part was cleaved off from the recombinant protein using PreScission Protease (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 µl of antiserum were coupled to 60 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed three times in CPB and supplemented with 6 µl fresh rPAS ...
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bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare, Mississauga, ON). Endotoxin was removed using the Pierce™ High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant protein MdSUMO2A-HIS and MdDREB2A were purified by HIS Sepharose beads (GE Healthcare, Fairfield, CT, USA), GST-MdCE was purfied by Pierce™ Glutathione Spin Columns (16105 ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system as follows (GE Healthcare). First ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins were purified by nickel affinity chromatography using HisTrap Column (GE Healthcare Life Sciences Pittsburgh, PA). Rat polyclonal antisera against L ...
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bioRxiv - Molecular Biology 2020Quote: ... 250 μl of antiserum were coupled to 50 μl of recombinant protein A-sepharose (rPAS, GE Healthcare). Antibody beads were washed three times in PBS and treated with 5 μl fresh rPAS ...
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysisbioRxiv - Biophysics 2021Quote: ... Recombinant D9 was purified sequentially by a Ni-NTA column followed by a heparin column (GE Healthcare) and a GE Superdex 75 16/60 gel filtration column to separate aggregates and exchange into crystallization buffer (10 mM MES pH 5.6 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant CrRPE1 was injected on a Superose 6 Increase 10/300 GL column (GE Healthcare, Chicago, Illinois) and isocratically eluted in 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant protein GST-LSH10 was purified using Glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant CaM was purified using phenyl-sepharose resin (Phenyl Sepharose® 6 Fast Flow, GE Healthcare Inc.) as described (Bender et al. ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 170 µl of antiserum was coupled to 40 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed extensively in CPB and supplemented with 4 µl fresh rPAS ...
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bioRxiv - Microbiology 2024Quote: Recombinant WT and mutant IN proteins were analyzed using Superdex 200 10/300 GL column (GE Healthcare) with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Molecular Biology 2021Quote: The binding affinity of the discovered antibodies for α-cobratoxin was determined using Surface Plasmon Resonance (SPR) (BIAcore T100, GE Healthcare). All measurements were performed at 25 °C using 10 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... Kinetic analysis was performed on each injection series using a 1:1 (Langmuir) binding model was applied using Biacore T200 Evaluation Software (GE Healthcare). Equilibrium dissociation constants (KD ...
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bioRxiv - Biochemistry 2022Quote: ... including a reference channel for online background subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface (Biacore T100 Control Software; GE Healthcare). All measurements were performed with 2-fold serial dilutions using 60 s association followed by a dissociation time of more than 240 s at 25 °C with a flow rate of at 30 μl min−1 ...
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bioRxiv - Immunology 2021Quote: ... PPACK-thrombin or fibrinogen in acetate buffer pH4.5 was immobilized to a chip surface (1000 or 10000 RU) using NHS/EDC-amine coupling and residual binding sites blocked by 1M ethanolamine (GE Healthcare). FH (0.5-62nM ...
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bioRxiv - Biophysics 2019Quote: ... Kinetic constants were determined by fitting 1:1 Langmuir interaction model to the binding curves in the Biacore T200 Evaluation Software (GE Healthcare). Analysis of all A/P variants were restricted to concentrations of 25nM and lower ...
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bioRxiv - Microbiology 2021Quote: Kinetic measurements of the DH1047 Fab binding to SARS-CoV and RsSHC014 spike proteins were obtained using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Microbiology 2021Quote: ... assay was performed to determine the binding affinities of aCPSF1 to poly-U-tract containing RNA on a BIAcore 8K instrument (GE Healthcare) as described previously (Zhang et al. ...
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bioRxiv - Biophysics 2022Quote: Binding kinetics of ACE2 and SARS-CoV-2 RBDs were determined by surface plasmon resonance using a Biacore S200 (GE Healthcare). All experiments were performed in a running buffer composed of 10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Biochemistry 2022Quote: Antibody binding to SARS-CoV-2 spike was assessed using SPR on a Biacore T-200 (Cytiva, MA, formerly GE Healthcare) with HBS buffer supplemented with 3 mM EDTA and 0.05% surfactant P-20 (HBS-EP+ ...
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bioRxiv - Immunology 2020Quote: ... Ab binding (protein expression) was visualized using Amersham ECL Prime Western blotting detection reagent (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). The following Abs were used in this study ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then diluted 10 times with mono Q binding buffer (50 mM HEPES, pH 8.0) and applied to a MonoQ 5/50 GL column (GE Life Sciences) connected to an AKTA Pure system (GE Life Sciences). ...