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Citations for GE Life Sciences :
251 - 300 of 948 citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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bioRxiv - Microbiology 2020Quote: Binding affinities were measured by isothermal titration calorimetry (ITC) using a MicroCal iTC200 system (GE Healthcare) at 25°C using wild type truncated SPPV14 as well as two mutants SPPV14 Y46A and R84A in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Analyte to ligand binding experiments were done in the Biacore 3000 instrument (GE Healthcare Life Sciences) at 25°C ...
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bioRxiv - Microbiology 2022Quote: ... The binding affinity was determined by and curves were generated by BIAEvaluation software (GE Healthcare, USA).
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bioRxiv - Cell Biology 2022Quote: ... RNA was purified using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) and was stored with RNAse-inhibitor at −80°C.
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bioRxiv - Immunology 2020Quote: ... The sensograms were fit in a 1:1 binding model with BIA Evaluation software (GE Healthcare). For epitope mapping ...
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bioRxiv - Neuroscience 2022Quote: ... The binding of HRP-conjugated secondary antibodies was revealed by ECL Plus (GE Healthcare Life Sciences) and chemiluminescence was measured using a ChemiDoc XRS apparatus (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... Antibody binding was visualized with Amersham ECL Western blotting detection reagent (GE Healthcare, Little Chalfont, UK).
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bioRxiv - Biochemistry 2021Quote: ... Binding of phage or FLAG-tagged UbVs was detected using anti-M13-HRP (GE Healthcare 27942101) or anti-FLAG-HRP antibody (Sigma-Aldrich A8592) ...
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bioRxiv - Cell Biology 2022Quote: Binding experiments and kinetics assays were carried out using a BIACORE 3000 instrument (GE Healthcare, USA). Two different sensor chips were used ...
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bioRxiv - Biochemistry 2023Quote: SEC binding assays were performed using a Superdex 200 increase Hiload 10/300 column (GE healthcare) at a flow rate 0.4 ml/min with absorbances monitored at 280 nm ...
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bioRxiv - Biochemistry 2023Quote: All the binding experiments were performed using the Superdex 75increase 10/300 GL column (GE Healthcare). 500 µl of reaction volume containing 20 µM Phospho-Ub (CBD tag)/phospho-UbN60D (CBD tag)/phospho-NEDD8 (His-SUMO tag ...
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bioRxiv - Cell Biology 2023Quote: ... The antibody binding was visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE healthcare; #RPN2236) and imaged with the ImageQuant LAS400 Imaging unit (GE Healthcare).
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... the recombinant protein was purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant proteins were first purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange using HiPrep 26/10 desalting column (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The recombinant proteins were purified by Ni+ sepharose 6 fast flow (GE Healthcare), glutathione Sepharose 4B (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant tau proteins were immobilized on the CM5 sensor chip (Biacore, GE Healthcare) via amine coupling ...
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bioRxiv - Immunology 2021Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). For IgA1 or IgA2 antibodies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The recombinant protein was purified through a nickel-nitrilotriacetic acid column (GE Healthcare) and subsequently loaded on a gel filtration column Sephacryl S-200 HR (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Purified recombinant proteins were loaded onto Superdex 75 10/300 column (GE Healthcare) in 150mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified by Ni2+-IMAC on HisTrap columns (GE Healthcare). Fractions ...
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bioRxiv - Immunology 2020Quote: ... The recombinant GST fusion proteins were affinity purified on GSH-Sepharose (GE Healthcare) from bacterial cultures incubated with 0.25 mM isopropyl-β-D-thiogalactopyranoside (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant proteins were purified with a fast flow Ni-NTA column (GE Healthcare) and eluted by 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant CoxbHtrA protein was purified using an ÄKTA avant system (GE Healthcare) equipped with a 5 mL StrepTrap HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: Recombinant RAD51 proteins were spotted on nitrocellulose membrane (GE Healthcare, Little Chalfont, UK). Membranes saturation ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). To produce antibody Fabs ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2024Quote: ... recombinant IgLON5 or LG1 protein was spotted onto a nitrocellulose membrane (GE Healthcare), membranes were blocked with 1% BSA in PBS ...
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
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bioRxiv - Molecular Biology 2023Quote: ... WT and C111S constructs were extracted from harvested cells by homogenization and soluble fractions loaded on a 5 ml HisTrap FF Crude column (GE Healthcare Life Sciences) equilibrated in binding buffer [20 mM Tris pH8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Response curves were fit to a 1:1 binding model using Biacore X100 Evaluation Software (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... The resulting data were fitted to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare). Also the ACE2 was immobilized onto CM5 chip and Serial dilutions of purified WT ...
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bioRxiv - Microbiology 2021Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Both kinetic and equilibrium binding data were analysed in Biacore 8K evaluation software version 1.1 (GE Healthcare). Equilibrium binding measurements were taken once equilibrium had been reached using reference-subtracted sensorgrams ...
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bioRxiv - Cell Biology 2021Quote: ... Sites of primary antibody binding were determined using an enhanced chemiluminescence system (GE Healthcare, Pittsburgh, PA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2022Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Developmental Biology 2019Quote: The single cycle kinetics method was used for sFLT1 binding assay by Biacore (GE Healthcare Bio-Science). A CM5 series S sensor chip (GE Healthcare Bio-Science ...
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bioRxiv - Microbiology 2020Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Kinetic constants were determined by the 1:1 Langmuir binding model using Biacore T200 software (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Binding of GAPDH proteins to EV surface was monitored by UV-280 chromatogram (Akta pure, GE Healthcare), western blotting ...
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bioRxiv - Biochemistry 2020Quote: The antibodies binding properties were analyzed at 25 °C on a Biacore ™ 8K instrument (GE Healthcare) using 10mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: Calorimetric binding experiments of Tim23IMS and TIM chaperones were performed using a MicroCal ITC200 instrument (GE Healthcare). Sixteen successive 2.5 µl aliquots of 1.15 mM Tim23IMS were injected into a sample cell containing 55 µM TIM9·10 or TIM8·13 ...
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bioRxiv - Immunology 2021Quote: ... The binding experiments were performed at 25°C on a Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) in HBS-EP+ buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The resulting data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare). His-tagged SARS-CoV RBD-SD1 was immobilized to an NiNTA sensorchip to a level of ∼350 RUs using a Biacore X100 and the same running buffer listed above ...