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Citations for GE Life Sciences :
1 - 50 of 948 citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing soluble recombinant protein were pulled-down with Glutathione (GSH) Sepharose 4FastFlow (GE Healthcare, 17-5132) for 3h at 4°C with gently rocking ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: Filtered soluble recombinant wild-type RBPMS-A and mutants were purified on an AKTA protein purification system (GE Healthcare;). Recombinant protein was first purified on HisTrap HP column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Disulfide bond-linked soluble TCRs were produced as previously described.11 The binding between the soluble TCR and pMHC was determined using surface plasmon resonance (SPR) on a Biacore T200 (GE Healthcare) as previously described.11
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... drained matrix (Lentil Lectin Sepharose 4B, GE Healthcare) in a 1:1 sample-to-matrix ratio ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... assay was performed to quantify the binding avidity of the recombinant mos-tri-RBD to the receptor hACE2 using BIAcore 8K (GE Healthcare) with NTA chips ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
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bioRxiv - Biochemistry 2019Quote: ... 75 μL of soluble thrombin (GE Life Sciences) was added to the dialyzed protein for an overnight incubation at 4°C ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Biophysics 2022Quote: ... Lectins were labeled with Cy5 mono-reactive dye (NHS ester) from GE Healthcare. Briefly ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Molecular Biology 2021Quote: ... The soluble portion was incubated with glutathione sepharose (GE healthcare) beads for 3-4 hours at 4°C and eluted with glutathione ...
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bioRxiv - Microbiology 2020Quote: ... Soluble proteins were purified using HisTrapTM Excel (GE Healthcare, Poland) column in buffer A with a linear gradient of 0-100% of 1 M imidazole in buffer A in 20 column volumes ...
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bioRxiv - Biochemistry 2019Quote: ... soluble proteins were applied to a HisTrap-FF column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Cancer Biology 2020Quote: Soluble fractions were loaded onto an ÄKTA purification system (GE Healthcare) using an equilibrated HisTrap or GSTrap (both GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The binding curves were analysed using a kinetic binding model supplied with Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... the soluble lysate was loaded onto 1 mL Nickel beads (GE healthcare). The column was washed with lysis buffer containing 15 mM imidazole and his-m6A-Tracer was eluted by 250 mM imidazole in lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The soluble fraction was loaded on 1 mL Nickel beads (GE Healthcare). Beads were washed with lysis buffer containing 20 mM imidazole before protein was eluted by 250 mM imidazol in lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... soluble 6His-tagged proteins were purified using a NiNTA resine (GE Healthcare) and imidazole was removed by dialysis into a buffer containing 50 mM TrisHCl ...
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bioRxiv - Developmental Biology 2022Quote: ... from which soluble forms were purified on glutathione-Sepharose beads (GE Healthcare). Recombinant C-terminally HA tagged Su(H ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fraction was bound to the GST Sepharose 4B (GE Healthcare) for 90 to 120 min at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Soluble proteins were purified using GSH-Sepharose 4B (GE Healthcare Life Sciences) and size exclusion chromatography (High Load 16/60 Superdex 200 ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was purified using an ÄKTAxpress chromatography system (GE Healthcare). The sample was purified firstly using a 1 mL HisTrap crude column (GE Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble fraction was loaded onto a nickel column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Pathology 2020Quote: ... and binding detected using streptavidin-HRP (GE Healthcare) diluted 1:4000 in 50μL EB ...
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bioRxiv - Bioengineering 2024Quote: ... Binding curves were fitted using BIAevaluation (GE Healthcare) 1:1 binding kinetics to calculate KD.
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bioRxiv - Biochemistry 2021Quote: ... the soluble fraction was applied to a HisTrap FF NiNTA column (GE Healthcare) and washed first with high-salt buffer B (2 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble extract cleared of cellular debris was incubated with IgG sephorose (GE Healthcare) for 2h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2021Quote: ... The soluble protein was purified by absorption to Ni2+-NTA resin (GE Healthcare), washed with Ni-buffer A (20 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble fraction was loaded onto a nickel column (GE Healthcare, USA) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... The soluble fraction was loaded onto a HisTrap HP 5ml column (GE Healthcare), pre-equilibrated with buffer B and then washed with 6 volumes of buffer B supplemented with 30 mM imidazole ...
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bioRxiv - Biophysics 2019Quote: ... The soluble material was purified and loaded on Ni2+-sepharose resin (GE Healthcare) in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... soluble proteins were loaded onto a Ni-column (HisTrap FF Crude, GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... and the soluble lysate was purified over a glutathione affinity column (GE Healthcare). Bound complex was eluted via overnight on-column TEV cleavage to remove the GST-and His-tags ...
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bioRxiv - Biochemistry 2021Quote: ... GST-β2HA was purified from the soluble fraction using glutathione resin (GE Healthcare). The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was loaded onto a 5 ml HisTrap column (GE Healthcare), which was preequilibrated with the Lysis buffer plus 20 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... the soluble lysate was loaded onto a HiTrap XL SP column (GE Healthcare) for cation exchange chromatography and eluted with a 0-0.5 M NaCl gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was subsequently incubated with 2 ml Ni-NTA (GE Healthcare) per 1,000 ODs for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Background binding to reference flow cells was subtracted and antibody binding levels were calculated using Biacore T200 evaluation software (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... The binding affinity was determined by global fitting to a Langmuir 1:1 binding model within the Biacore Evaluation software (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was incubated with Nickel Sepharose 6 Fast Flow beads (GE Healthcare) for 30 minutes at 4 °C in batch ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was incubated with Ni Sepharose 6 Fast Flow beads (GE Healthcare) for 30min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was incubated with Ni Sepharose 6 Fast Flow beads (GE Healthcare) for 30 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... the soluble fraction was incubated with Ni Sepharose 6 Fast Flow beads (GE Healthcare) for 30min on ice ...