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Citations for GE Life Sciences :
1351 - 1400 of 3790 citations for Mono 2 Ethyl 5 Carboxypentyl Phthalate 90% Pure 13C4 99% Dehp Metabolite V ;100Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to 5 or 10 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (20 mM Tris-HCl ...
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bioRxiv - Genetics 2019Quote: The clarified lysate containing mZCWPW1 was then loaded onto a 5 mL His-Trap HP column (GE Healthcare) using NGCTM chromatography system (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was added to a 5 mL Ni-Sepharose fast-flow resin (GE Healthcare Life Sciences) pre-equilibrated with buffer A at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... We then applied the supernatant onto two tandem 5 mL HiTrap Q HP anion exchange columns (GE Healthcare) pre-equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: The PCR product was purified via ion exchange chromatography using a 5 ml HiTrap Q column (GE Healthcare). The column was equilibrated in Buffer A (25 mM HEPES 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Then the solution was injected onto a prewashed SP Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL). The column was washed with 20 CV of Buffer D (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Genetics 2023Quote: ... for 45 min and soluble extract was applied to a 5 ml HisTrap FF column (GE healthcare Inc.) using a GE AKTA Avant 25 FPLC system ...
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bioRxiv - Microbiology 2023Quote: ... proteins were separated by immobilized-metal affinity chromatography (IMAC) on a 5 mL HisTrap HP column (GE Healthcare), previously washed with ddH2O and equilibrated with buffer B (25 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then transferred to a 5 mL tube containing 300 μL CaM-Sepharose resin (GE Healthcare) equilibrated in 50 mM HEPES (pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
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bioRxiv - Biophysics 2023Quote: ... centrifuged and applied to a cation exchange chromatography column (5 mL HiTrap SP HP, GE Healthcare Life Science) equilibrated with 25 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
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bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluents were pooled and directly loaded onto HiTrap Q HP 5 mL anion exchange column (GE Healthcare) pre-equilibrated with buffer D [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... The dialyzed solution was then loaded on a 5 ml HiTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... to a polyvinylidene difluoride membrane and blocked in TBST buffer (TBS with 0.1% (v/v) Tween-20) supplemented with 1% ECL Advance Blocking Agent (GE Healthcare, Waukesha, WI) for 40 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Prior to infection HFFs were grown to confluency in DME supplemented with 10% v/v cosmic calf serum (GE Healthcare, New Zealand) (D10) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was diluted 1:10 v/v with 20 mM Tris 8.0 and further purified with anion exchange chromatography (HiTrapQ column, GE Healthcare Life Sciences) using a NaCl gradient of 50 mM to 500 mM in 20 mM Tris 8.0 ...
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bioRxiv - Immunology 2022Quote: ... GPC-I53-50A was concentrated to at least 2 µg/ml using Vivaspin centrifugal filters (GE Healthcare). To generate complexes ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...
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bioRxiv - Biophysics 2021Quote: ... The purification was done by purifying these two supernatants with 1 M NaCl containing Tris buffer using Hi-Trap Q-FF anion exchange column of ÄKTA Pure (GE healthcare). To maintain the oxidation activity of DsbA 0.3% H2O2 was added to the purified protein for overnight ...
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bioRxiv - Biophysics 2021Quote: ... Then the cells were disrupted in French press and the lysate was extracted and purified using Ni2+-NTA affinity column of [KTA Pure (GE healthcare). We used 20 mM imidazole containing buffer as binding buffer and 250 mM imidazole containing sodium phosphate buffer as elution buffer ...
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bioRxiv - Biophysics 2020Quote: ... the solution was loaded onto a CM-Sepharose column coupled to an Äkta Pure system (GE Healthcare Life Sciences, Brøndby, Denmark). The sample was loaded on a column equilibrated with buffer A (50 mM Tris-HCL ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Cell Biology 2020Quote: ... and KDM5A F9ΔPID were cleaved from MBP with Factor Xa and further purified using HiTrap SP HP column (GE29-0513-24) on Akta Pure FPLC (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl, 20 mM NaH2PO4, 500 mM imidazole) on an ÄKTA Pure chromatography system (GE Healthcare). Fractions containing the protein were pooled ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated TANV16L was subjected to size-exclusion chromatography using a Superdex S200 increase 10/300 column mounted on an ÄKTA Pure system (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted protein was passed through a HiLoad 16/600 Superdex 200 PG size exclusion column using an ÄKTA pure purification system (GE Healthcare). Peak fractions were pooled and concentrated using Amicon Ultra-4 centrifugal filter units (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted protein was passed through a HiLoad 10/300 Superdex 75 GL size exclusion column using an ÄKTA pure purification system (GE Healthcare). Peak fractions were pooled and concentrated using Amicon Ultra-4 centrifugal filter units (Millipore) ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was eluted by using purification buffer including 200 mM imidazole and further purified by gel chromatography on an ÄKTA pure system equipped with a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) running with purification buffer ...
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bioRxiv - Physiology 2019Quote: We purified individual isoHbs by means of anion-exchange fast-protein liquid chromatography (FPLC) using an Äkta Pure system (GE Healthcare). Stripped hemolysate was loaded on the prepacked HiTrap Q-HP column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and Waters 410 differential refractometer (Waters) in line with an ÄKTA pure chromatography system fitted with a Superdex 200 Increase column (both GE Healthcare). Data was analysed using ASTRA 5.3.4 software (Wyatt Technology).
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bioRxiv - Biochemistry 2020Quote: ... then followed by size exclusion with either a Superdex 75 or Superdex 200 column in an AKTA pure FPLC (GE Healthcare). The final protein product was stored in buffer containing 20 mM Tris pH 7.3 ...
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bioRxiv - Biophysics 2021Quote: ... 500 mM imidazole) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex 75 Increase 10/300 GL column, GE Healthcare). Protein concentration is determined using the protein extinction coefficient and corrected for direct absorption of the fluorophores at 280 nm ...
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bioRxiv - Biochemistry 2021Quote: ... Purification of enzyme having N-terminal hexahistidine tag was done by Ni-NTA affinity chromatography using an Akta pure FPLC system (GE Healthcare). Equilibration buffer was 50 mM tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were cut overnight at 4°C with 6X-His tagged ULP Sumo protease followed by gel filtration chromatography using a Superdex 200 gel filtration column attached to an AKTA Pure FPLC chromatography system (GE Healthcare). Peak fractions were collected and concentrated ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... connected to a ÄKTA Pure apparatus equipped with a UV 280 nm flow cell (all from GE Healthcare, Chicago, IL, USA). EV fractions were collected based on UV absorbance ...
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bioRxiv - Microbiology 2020Quote: ... Purified protein obtained using glutathione affinity chromatography was then subjected to size-exclusion chromatography using a Superdex S75 10/300 or 16/600 column mounted on an ÄKTA Pure system (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted protein was then supplemented with 1mM TCEP and concentrated to be further purified by gel filtration on a HiLoad Superdex200 16/600 using an ÄKTA Pure (GE Healthcare). The gel filtration column was pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove soluble aggregates ...
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bioRxiv - Cell Biology 2020Quote: ... The individual TMT labeled samples were pooled and then fractionated using strong cation exchange chromatography on an AKTA Pure 10 (GE Healthcare) equipped with a Luna 5 μm 100 angstrom 150 x 2.1 mm strong cation exchange (SCX ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein eluted with a gradient from 20 to 500 mM imidazole over 10 column volumes using an AKTA Pure (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... the protein samples were then further purified using size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (ÄKTA pure, GE Healthcare). The desired fractions containing dimeric VP40 or monomeric LactC2 were collected ...
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bioRxiv - Immunology 2022Quote: ... The secreted protein was purified from pre-conditioned media supernatant with Ni-NTA resin and purified via size exclusion chromatography with a S200 increase column on an AKTA PURE FPLC (GE Healthcare). To improve protein yields ...
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bioRxiv - Biochemistry 2022Quote: All chromatography purification steps were carried out at 4°C using an ÄKTA pure fast protein liquid chromatography (FPLC) system (GE Healthcare). All buffer solutions were chilled prior to use ...
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bioRxiv - Bioengineering 2022Quote: ... and the supernatant containing the soluble fraction was collected and filtered sterilized before purification in the ÄKTA Pure chromatograph (GE Healthcare).