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Citations for GE Life Sciences :
1551 - 1600 of 3790 citations for Mono 2 Ethyl 5 Carboxypentyl Phthalate 90% Pure 13C4 99% Dehp Metabolite V ;100Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... nickel affinity chromatography was performed where the lysate was loaded onto a 5 mL HisTrap™ Excel column (GE Healthcare), followed by a wash with 20 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: Single cysteine mutants of ColE9 or the ColE9 DNase domain were desalted on a 5 ml HiTrap column (GE Healthcare) equilibrated with binding buffer (20 mM potassium phosphate pH 7 ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble cell lysate obtained after centrifugation was loaded onto a 5 ml HisTrap FF HP nickel column (GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Biochemistry 2020Quote: ... The co-expressed complex of ctCyclin H and ctMAT1short was loaded on a 5 ml StrepTrap HP column (GE Healthcare) equilibrated in lysis buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was centrifuged at 38,000 ×g and the supernatant was loaded onto a 5 mL HisTrap Ni-NTA column (GE Healthcare). The column was washed with HisTrap Buffer and protein eluted with a linear gradient of imidazole from 25 to 300 mM in HisTrap Buffer over 10 column volumes.
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bioRxiv - Systems Biology 2021Quote: ... The supernatants obtained after this step were loaded in 5-mL His-trap columns (GE Healthcare Systems, Chicago, IL, USA) pre-equilibrated with buffer A supplemented with 100 mM NaCl and 20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was filtrated through a O.22μm pore filter and loaded on a 5 ml pre-packed Hitrap Q FF column (GE Healthcare) equilibrated with buffer A (50mM bis-tris-HCI pH 6.0 ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was subjected to immobilized-metal affinity chromatography (IMAC) using a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB1 containing 20 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation for 30 min at 47,000 xg and the supernatant was loaded onto a 5 ml GSTrap column (GE Healthcare). The resin was washed by the lysis buffer and bound protein was eluted with elution buffer of 100 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Human IgGs were isolated from heat-inactivated plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) as described (24).
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bioRxiv - Cell Biology 2021Quote: ... The lysate was cleared by centrifugation and it was applied to a 5 ml GSTrap FF column (GE Healthcare, 17513101) to purify GST-proteins ...
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bioRxiv - Cell Biology 2021Quote: ... His-Bro1ΔAPRR purified from BL21-DE3 using Ni2+-affinity chromatography (5 ml HiTrap Chelating FF column; GE Healthcare, United Kingdom). GST-Bro1V(370-709 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation for 1 hour at 38,700 x g and passed over a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 15 column volumes of 20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM TCEP) and the sample was loaded onto a 5 mL HiTrap Heparin HP affinity column (GE healthcare) pre-equilibrated with 5% buffer B (50 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was consecutively filtered through 0.45 µm and 0.22 µm PES syringe filters prior to loading onto a 5 ml HisTrap nickel affinity column (GE Healthcare) preequilibrated with Buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
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bioRxiv - Biochemistry 2022Quote: ... The eluate of HIC-EBA was subjected to affinity chromatography using Strep-Tactin® column (5 ml cartridge, GE Healthcare Life Science ...
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bioRxiv - Biochemistry 2022Quote: ... to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare). A linear 0-75 mM histidine gradient with elution buffer was used to elute proMbxA ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant of the lysate was filtered through a 0.22 µm membrane filter and loaded onto a Ni-NTA column (HiTrap, 5 ml, GE Healthcare) using a low imidazole buffer (Buffer C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered using a 0.2 μl filter and flown over a 5 ml NiNTA IMAC column (GE Healthcare) equilibrated with buffer A (50 mM KH2PO4 pH 7 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered using a 0.2 μl filter and flown over a 5 ml StrepTactin-Sepharose column (GE Healthcare) equilibrated with buffer A (50 mM MES-NaOH ...
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bioRxiv - Microbiology 2022Quote: ... and the supernatant was filtered using a 0.2 μl filter and flown over a 5 ml NiNTA Immobilised Metal Affinity Chromatography (IMAC) column (GE Healthcare) equilibrated with buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged and the supernatant was filtered through a 0.45 μm filter and subjected to a His-tag affinity chromatography using a 5 mL HisTrapFF (GE Healthcare) and the ÄKTAprime plus system (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was supplemented with 50 mM imidazole and loaded onto a 5 mL His-Trap FF column (GE Healthcare), washed with 6 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mL imidazol gradient) or by size exclusion chromatography (SEC) using a Superdex® 75 10/300 GL (GE Healthcare) with a flow rate of 0.5 mL/min and dialysis buffer as running buffer ...
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was filtered using a 0.2 μM filter and loaded onto a 5 ml HisTrap HP Ni-affinity column (GE Healthcare). Protein was eluted in a gradient of 10 to 300 mM imidazole over five column volumes ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 mL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing MBP-AIM2 were pooled and purified by ion-exchange chromatography using 5 mL HiTrap-SP column (GE Healthcare) equilibrated in 20 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... The lysed cells were centrifuged and only the supernatant with the soluble proteins was then further filtered with 0.45 µm filters and loaded onto two connecting 5 mL HisTrap IMAC FF columns (GE Healthcare) which were pre-equilibrated with 50mM phosphate buffer with 500 mM NaCl and 20 mM imidazole at pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... to remove cell debris the supernatant was filtered through a 0.45-μm pore size filter and then loaded onto a 5-ml Ni-HiTrap column (GE Healthcare) that had been pre-equilibrated in the same lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was cleared by centrifugation at 100,000 x g for 45 min and loaded onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed extensively with NiA buffer ...
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bioRxiv - Biochemistry 2019Quote: ... the filtrate was loaded on an anion exchange HiTrap Q HP 5 ml column (GE healthcare life sciences, Boston, Massachusetts), at a flow rate of 1 ml/min ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation for 45 min at 48.000 xg and loaded onto a HisTrap FF 5-ml column (GE Healthcare). Eluted proteins were incubated with TEV protease at 4 °C overnight and both the His6-tag ...
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bioRxiv - Plant Biology 2020Quote: ... 20 mM imidazole pH 7.5 was added and the solubilized membranes were loaded on a pre-equilibrated 5 ml Ni-NTA column (GE Healthcare) at 3 ml/minute ...
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bioRxiv - Microbiology 2021Quote: ... The eluate was then diluted 3x with 20 mM Tris-HCl pH7.5 and passed over a 5 mL Q-sepharose column (GE Healthcare). The Flow through containing the MIF protein was then again loaded on a 5 mL CM sepharose column ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Biochemistry 2020Quote: ... Sample was filtered through a 0.45 μm nitrocellulose filter and loaded onto a 5 ml HisWash1-equilibrated HisTrap HP column (GE Healthcare). 2 ml fractions were collected through a linear gradient over 100ml up to 100% HisElution [50 mM Tris ...
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bioRxiv - Biophysics 2019Quote: ... The cleaved protein was then purified by a 5 ml HisTrap HP column on an AKTA Avant system (GE Healthcare). The flow through was combined and diluted with buffer C ...
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... Precipitated DNA was pelleted by centrifugation and soluble lysate was loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 7 column volumes of loading buffer before being eluted with a gradient of imidazole (20–500 mM ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was filtered through a 0.22-μm filter and loaded onto a 5 mL His Trap HP column (GE Healthcare) pre-equilibrated with buffer A (10 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was filtered and the model degrons were loaded on a HiTrap SP HP 5 ml column (GE Healthcare) in 50 mM ammonium acetate pH 4.5 ...
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bioRxiv - Biochemistry 2021Quote: ... then the soluble fraction was filtered through 0.22 μm PVDF membrane before loading onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 20 column volumes of 50 mM Tris-HCl pH 7.0 ...