Labshake search
Citations for GE Life Sciences :
1151 - 1200 of 3790 citations for Mono 2 Ethyl 5 Carboxypentyl Phthalate 90% Pure 13C4 99% Dehp Metabolite V ;100Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... The supernatant of the cell lysate was loaded onto a HisTrap column (5 mL-GE Healthcare) and the bound protein was eluted with an imidazole gradient (0-1 M) ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatants were initially applied to a HisTrap FF crude 5 mL (GE Healthcare UK Ltd.), which were pre-equilibrated with the HisTrap binding buffer (20 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare) equilibrated in 200 mM potassium phosphate ...
-
bioRxiv - Biophysics 2023Quote: ... The filtered supernatant was loaded onto a HisTrap FF 5 mL (GE Healthcare, Marlborough, MA, USA) preequilibrated with 50 mM Tris-HCl (pH 8.0 ...
-
bioRxiv - Microbiology 2023Quote: ... then poured onto loaded onto a pre-equilibrated HiTrap DEAE FF 5 ml column (GE Healthcare) into Buffer B (20 mM Tris pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... Eluted fractions were pooled and loaded onto a 5 ml anionic exchanger (Q-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied at 5 ml/min over 20 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... the clarified lysate was added to a 5 ml HisTrap High Performance column (GE Healthcare/Cytiva), washed with 5 column volumes of lysis buffer supplanted with 6 M urea ...
-
bioRxiv - Cell Biology 2022Quote: ... Eluted fractions were pooled and loaded onto a 5 ml anionic exchanger (Q- column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
-
bioRxiv - Biochemistry 2023Quote: ... The cell extract was applied onto a HisTrap™ FF crude column (5 ml; GE Healthcare) pre-equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Microbiology 2023Quote: ... then supernatant was loaded onto a 5 mL Ni2+ affinity column (HisTrap HP, GE Life Sciences) pre-equilibrated with resuspension buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... Supernatant was then circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) that was inoculated with 100mM CoCl2 for ten minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
-
bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on a 5 ml HiTrap Q HP column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...
-
bioRxiv - Genomics 2019Quote: ... The flow-through was then loaded onto a 2 mL HiTrap SP column (GE Healthcare) in 17% IEX-B buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... loaded in a gravity column containing 2 mL of pre-equilibrated Ni-sepharose (GE Healthcare), and incubated under slow rotation for 30 min at 8 °C ...
-
bioRxiv - Immunology 2022Quote: ... was synthesized by ABclonal Technology and got the 2 mg/mL purified antigen through Ni-IDA (GE Healthcare, USA). The mouse polyclonal antibodies against two parts of PGRP-S2 (NM_001163716.1) ...
-
bioRxiv - Cell Biology 2023Quote: ... Forty 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... 40 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
-
bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 mL of Glutathione-Sepharose resin (GE Healthcare) and incubated for 1 hour at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... acetonitrile (ACN) and 0.1% (v/v) trifluoro acetic acid (TFA) using a Superdex Peptide PC 3.2/30 column (GE Healthcare). After evaporating to dryness and dissolving in 4% (v/v ...
-
bioRxiv - Bioengineering 2020Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
-
bioRxiv - Microbiology 2020Quote: ... All assays were performed with a running buffer of PBS pH 7.4 supplemented with 0.005% v/v Surfactant P20 (GE Healthcare) at 25 °C ...
-
bioRxiv - Bioengineering 2020Quote: ... at room temperature with HBS-EP+ running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). IgGs were immobilized on CM5 chips (GE Healthcare # 29104988 ...
-
bioRxiv - Microbiology 2022Quote: ... the purified paLptDE or sfLptDE protein samples were buffer exchanged into 150 mM ammonium acetate (pH 8.0) containing 0.5% C8E4 (v/v) on a Superdex 200 gel filtration column (GE Healthcare). LptDE samples were subsequently introduced via gold-coated nanospray capillaries prepared in house and MS spectra were recorded on a Synapt G2-Si instrument (Waters ...
-
bioRxiv - Bioengineering 2023Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
-
bioRxiv - Bioengineering 2024Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrapTM HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
-
bioRxiv - Cell Biology 2020Quote: ... Size exclusion chromatography was performed in storage buffer at room temperature on an ÄKTA Pure 25L1 system (GE Healthcare). Elution of proteins was monitored at 280 nm ...
-
bioRxiv - Microbiology 2021Quote: ... and further purified using size exclusion chromatography (SEC) on an AKTA pure 25L protein purification system (GE Healthcare, USA) in buffer C (20 mM HEPES pH 7.5 ...
-
bioRxiv - Plant Biology 2021Quote: Multi-angle light scattering coupled size-exclusion chromatography (SEC-MALS) was performed using an Äkta PURE system (GE Healthcare) with a Superdex 200 Increase 10/300 column attached to a MALS detector 3609 (Postnova Analytics ...
-
bioRxiv - Physiology 2021Quote: ... Fractions containing >95% pure RyR1 were pooled and further purified with a HiTrap Heparin HP Agarose column (GE Healthcare), after a five-fold dilution and filtration step ...
-
bioRxiv - Plant Biology 2021Quote: ... soluble proteins were purified by affinity chromatography using a HisTrap Ni-NTA column (ÄKTA pure FPLC system, GE Healthcare) equilibrated in 50 mM Tris-HCl ...
-
bioRxiv - Biophysics 2021Quote: ... using an inline size-exclusion chromatography set-up (AKTA pure with Superdex 200 10/300 GL column; GE Healthcare). Two-dimensional scattering data were radially averaged ...
-
bioRxiv - Biochemistry 2022Quote: ... fractions containing pure protein sub-complexes were subjected to size-exclusion chromatography (Superose 6 increase 10/300, GE Healthcare) in 25 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2019Quote: RAD51 purification was carried out by chromatography at 4°C using the AKTA Pure Protein Purification System (GE Healthcare). For WT RAD51 ...
-
bioRxiv - Biochemistry 2019Quote: ... pH 7.0 using a Centri Pure Zetadex-25 gel filtration column (Generon) and labelled with Cy3B maleimide (GE Healthcare) at room temperature for 2 hours using a 5 times molar excess of Cy3B ...
-
bioRxiv - Synthetic Biology 2020Quote: ... All purifications were carried out on an Äkta™ start or Äkta™ pure chromatography system (GE Healthcare, USA). For the purification of free mSOG the unbound fraction obtained during the IMAC purification of Enc-mSOG was used ...
-
bioRxiv - Immunology 2021Quote: ... The beads were then packed in a column coupled to an AKTA™ Pure system (GE Healthcare, Life Sciences). HypR was eluted using a linear gradient with elution buffer ...
-
bioRxiv - Microbiology 2019Quote: ... ClpP containing fractions were pooled and further purified by size exclusion chromatography (ÄKTA pure 25 M1 FPLC; GE Healthcare) using a Superdex 75 column (GE Healthcare ...
-
bioRxiv - Microbiology 2021Quote: ... size-exclusion (SEC) column connected to an ÄTKA™ pure fast protein liquid chromatography (FPLC) system (GE Healthcare Inc.). The column was equilibrated with SEC buffer (50 mM Tris ...
-
bioRxiv - Immunology 2020Quote: ... The trimer fraction was purified by size exclusion chromatography on a Superdex 200 10/300 GL column in TBS + 0.1% DDM + 0.003% sodium deoxycholate using an ÄKTA Pure 25L HPLC instrument (GE Healthcare) at a flow rate of 0.75 ml/min.
-
bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
-
Design and Evaluation of Nanoscale Materials with Programmed Responsivity towards Epigenetic EnzymesbioRxiv - Bioengineering 2024Quote: ... and pure HDAC8 enzyme was obtained using the HisTrap column on AKTA purifier UPC 10 (GE Healthcare Life Sciences). SDS-PAGE agarose gel and catalytic activity analysis confirmed the presence of the HDAC8 enzyme ...
-
bioRxiv - Biophysics 2023Quote: ... Buffer conductivity was measured on the built-in conductivity monitor of the chromatographic system AKTA Pure 150M (GE Healthcare). The sample was kept at 4 °C no more than 7 d ...
-
bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superose 6 Increase 10/300 GL column (Sigma-Aldrich ...
-
bioRxiv - Evolutionary Biology 2024Quote: We performed size-exclusion chromatography of His-tag purified coGFP variants on an Äkta Pure chromatography system (GE Healthcare), with a Superdex 75 10/300 Increase column (GE Healthcare ...
-
bioRxiv - Bioengineering 2024Quote: ... and fractionated by size-exclusion through three tandem superdex 200 increase columns on an Akta Pure FPLC (GE Healthcare). The siRNA content of each fraction was measured with a plate reader (Biotek Synergy H1 ...
-
bioRxiv - Neuroscience 2023Quote: ... The collected cell suspension was mixed with isotonic Percoll (GE Healthcare, 90% Percoll 10% 10x PBS, pH 7.2) at a final concentration of 22.5% and centrifuged at 800 g for 20 min without break at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrap™ HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...