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Citations for GE Life Sciences :
1301 - 1350 of 3790 citations for Mono 2 Ethyl 5 Carboxypentyl Phthalate 90% Pure 13C4 99% Dehp Metabolite V ;100Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The complex was applied onto superdex200 increase 10/300 GL column via fast protein liquid chromatography (AKTA Pure, GE Healthcare) with buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved complexes were eluted from Streptactin beads and further fractionated by a HiTrap heparin column (AKTA Pure, GE Healthcare) in buffer B (25 mM Tris·HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was stopped on ice and loaded into the equilibrated column connected to ÄKTA pure purification system (GE Healthcare) as described above ...
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bioRxiv - Microbiology 2022Quote: ... The protein was loaded onto a SourceQ 15Q 4.6/100 PE anion-exchange column using an Äkta pure 25L FPLC instrument (GE Healthcare). For all conjugates ...
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bioRxiv - Cell Biology 2024Quote: ... Eluates containing protein were concentrated to <500µL and size-exclusion was performed at 4°C using an ÄKTA Pure Protein Purification System (GE healthcare) and Superose® 6 Increase 100/300 GL column (Cytiva) ...
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bioRxiv - Biophysics 2023Quote: ... All proteins were further purified using size-exclusion chromatography (ÄKTA pure system, Superdex 75 Increase 10/300 GL, GE Healthcare). The purified protein was split into aliquots and stored at -80 °C prior to the measurements.
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and by passing through a 0.45 µm filter before loading it into a Ni-NTA column connected to an AKTA Pure instrument (GE Healthcare). After the protein binding to Ni-NTA beads ...
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bioRxiv - Biochemistry 2023Quote: ... in SEC buffer (20 mM Sodium-phosphate buffer pH 6.5, 50 mM NaCl) using an ÄKTA pure chromatography system (GE Healthcare). Eluted fractions were concentrated using Amicon® Ultra to 1 mM final concentration.
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bioRxiv - Plant Biology 2024Quote: ... and loaded to a gel filtration column Superdex 200 Increase 10/300 GL operated with AKTA Pure 25L (GE Healthcare). Gel filtration was performed in the Size Exclusion Chromatography (SEC ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol and 1 mM DTT prior to ion exchange chromatography using a Fast Flow SP column (GE Healthcare). Elution from the SPFF column was performed with a 100 mM to 1M gradient over 20 column volumes ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was then exchanged into lysis buffer supplemented with 0.15% w/v OGNG / 0.015% w/v CHS by passing over a pre-equilibrated Sephadex PD-10 desalting column (GE Life Sciences). TEV protease was added to the desalted protein at a weight ratio of 1:10 for overnight tag cleavage ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were separated on 12.5% (v/v) acrylamide gels of 25 cm x 21 cm x 1 mm using an Ettan DALTsix electrophoresis unit (GE Healthcare). The SDS-PAGE was first run at a current of 2 W/gel for 1 h and then at a constant current of 15 W/gel for 5 h ...
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Immunology 2024Quote: ... HBS-P+ buffer (10 mM HEPES pH 7.4, 0.15 M NaCl, 0.05% v/v P20 surfactant, (GE Healthcare, Marlborough, MA, USA) was used as the running buffer ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were then purified by cation exchange using a 5-mL HiTrap SP HP column (GE Healthcare) and the collected fractions analyzed by SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... The cleaved dCas9 protein was further purified with a 5 ml SP Sepharose HiTrap column (GE Life Sciences) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants containing the protein extracts were then applied onto a HisTrap FF 5 ml column (GE Healthcare) loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and Sov was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
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bioRxiv - Genomics 2019Quote: ... The diluted supernatants were applied to a 5 mL HiTrap heparin column (HiTrap™ Heparin HP, GE Healthcare) coupled to an ÄKTA purifier system (ÄKTA explorer ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification was done using an AKTA Purifier FPLC system on a HisTrap FF 5 ml column (GE Healthcare). The column was washed and equilibrated with 10 volumes of LB prior to sample loading ...
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bioRxiv - Biophysics 2022Quote: ... Supernatants were filtered by 0.45 μm and loaded onto a 5 mL HiTrap MabSelect SuRe column (GE Healthcare) equilibrated with COP Wash buffer (20 mM Hepes pH 7.4 and 500 mM NaCl) ...
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bioRxiv - Biochemistry 2021Quote: ... 30 min) and the supernatant was loaded onto a 5 mL HisTrap HP Ni Sepharose column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on a Ni-IMAC-HisTrap™ FF 5 mL column (GE Healthcare, Chicago, IL) at 3.5 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the complex at reasonable purity were loaded onto a 5 ml HiTrap Heparin column (GE Healthcare), and the bound protein was eluted with a gradient from buffer A to buffer B (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... and then loaded on to a HiTrap™ SP Sepharose HP column (5 ml/min, GE Life Sciences) followed in tandem by a HiTrap™ Q Sepharose HP column (5 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... supplemented with 20 mM imidazole and loaded onto a 5 mL HisTrap HP Ni2+ affinity column (GE Healthcare). After washing with several column volumes (CV ...
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bioRxiv - Biophysics 2021Quote: ... and the final product was obtained by running the solution through an 5 mL Hi-Trap (GE Healthcare) desalting column (Figure S1).
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His- Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2022Quote: Solubilized porins were loaded onto an anionic-exchange column (Hitrap HQ, 5 mL, GE Healthcare Life Science, France) and subjected to detergent exchange with 0.1 M MES buffer pH 6.5 complemented with 0.1% lauryldimethylamine-oxide (LDAO ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2022Quote: ... The top fractions after IMAC were pooled and desalted using a 5 ml HiTrap Desalting column (GE Healthcare) equilibrated with 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was added to 5 mL of equilibrated Glutathione Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were washed in a column 3 times with 50 mL of Buffer C (10 mM phosphate buffer pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... the α-Syn solution was subjected to anion exchange column (AEC) (HiTrap Q Hp 5 ml, GE healthcare) followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then applied onto a Hi Trap affinity (5 ml) (Ni2+) column (GE Healthcare Life Sciences) The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered and loaded onto a 5 mL HisTrap HP column (GE Life Sciences, now Cytiva), followed by washing [0.05 M Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved DEPTOR protein was then collected and applied to a 5 mL HiTrap Heparin HP column (GE Healthcare) equilibrated with HEP-A buffer (50 mM HEPES pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) and then loaded on a HiTrap Heparin column (CV, column volume 5 ml; GE Healthcare), equilibrated with buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... His6–Ub-tagged SbtB7001 was purified from cell lysate using a 5 ml HisTrap FF column (GE Healthcare): the pre-equilibrated and lysate-loaded column was washed with binding buffer (20 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysate was clarified by centrifugation and loaded onto a HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic pump ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins from the cleared cell lysate were captured in a HisTrap HP 5 ml column (GE Healthcare) in His buffers (20 mM MOPS ...
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bioRxiv - Microbiology 2019Quote: Purified capsids (100 µg) were loaded onto a 5-ml HiTrap™ Heparin-Sepharose HP Column (GE Healthcare), which then washed with Ca2+ and Mg2+-free PBS to remove unbound molecules and eluted with gradually linear increasing concentrations of NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the crude lysates were applied to 5 ml HiTrap Capto Q column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer A (20 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and LC8 was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
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bioRxiv - Pathology 2021Quote: ... diluted five-fold with extraction buffer and applied to a 5 ml HisTrap column (GE Healthcare, Chicago, IL) equilibrated with extraction buffer ...
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bioRxiv - Biophysics 2020Quote: ... The EcA2 used for these experiments were previously desalted in a 5-mL HiTrap Desalting (GE Healthcare, USA) and eluted in 10 mM sodium phosphate at pH 7.0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...