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Citations for GE Life Sciences :
9401 - 9450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Fractions containing DsbA were pooled and passed over a Superdex 200 26/60 gel filtration column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH ...
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bioRxiv - Biophysics 2022Quote: ... After loading the cell lysate onto a Protein L beads (Capto™ L, GE Healthcare, Cat. no. 17-5478-02) column ...
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bioRxiv - Biophysics 2022Quote: ... FimCA was loaded onto a Source 30S (GE Healthcare) column equilibrated with the same buffer and eluted with a linear gradient from 0-200 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... DTT was removed by desalting using a Sephadex G25 column (GE Healthcare) equilibrated in 3 M GdmCl ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded onto a 6 ml Resource Q column (GE Healthcare) equilibrated in 10 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... followed by concentration in a Vivaspin 20 concentrator [10-kDa MWCO (molecular weight cutoff)] and final gel filtration step on a HiLoad 16/600 Superdex 200 pg gel filtration column (GE Healthcare) equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... loaded onto a Source 30S (GE Healthcare) column equilibrated with the same buffer and eluted with a linear gradient from 0-300 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... samples were loaded at 4 °C onto a Resource S column (GE Healthcare) equilibrated in 20 mM MES-NaOH pH 6.0 (for reactions containing FimCHisI or FimCHisIA) ...
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bioRxiv - Biophysics 2022Quote: ... The refolding mixture was dialyzed at 4 °C against 20 mM MOPS-NaOH pH 6.7 to remove residual GdmCl and applied to a Resource S column (GE Healthcare) to separate FimCHisI from free FimCHis ...
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bioRxiv - Biophysics 2022Quote: ... concentrated by ultrafiltration and passed over a Superdex 75 26/60 column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 8.0 at room temperature) ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was desalted using a PD10 column (GE Healthcare, Cat. no. 17085101) in 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... concentrated by ultrafiltration and passed over a Superdex 75 16/60 column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 8.0 at room temperature) ...
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bioRxiv - Biophysics 2022Quote: ... the ternary complexes were purified by cation exchange chromatography on a Resource S column (GE Healthcare). The FimCHisIA complex was eluted with a linear NaCl gradient in 20 mM MOPS-NaOH pH 6.7 and eluted at ~200 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... proteins were concentrated by ultrafiltration at 4 °C (MWCO: 10 kDa) and passed over a Superdex 75 26/60 gel filtration column (GE Healthcare) equilibrated in 20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Biophysics 2022Quote: ... Calcein-filled liposomes were separated from free calcein using a PD-10 desalting column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... Secondary antibody staining using either sheep α-mouse or donkey α-rabbit (NA931V, NA934V, GE HealthCare, Chicago, IL, USA) was performed for 1 hour on a tube roller at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... at a 1:3 protein:dye molar ratio for 1 h at room temperature and then desalted on a PD-10 desalting column (GE Healthcare), where the free dye and the conjugated Alexa488-αSOs were separated ...
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bioRxiv - Biophysics 2022Quote: ... Assays were performed at 25 °C on a Biacore 2000 instrument (GE Healthcare). Regeneration of the surface to remove bound analyte was carried out using 0.005% SDS ...
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bioRxiv - Biophysics 2022Quote: ... The sensorgram data was evaluated using the Biacore evaluation software (GE Healthcare). The kinetic data was fitted to a 1:1 binding model to give the equilibrium dissociation constant Kd.
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bioRxiv - Biophysics 2022Quote: ... was diluted to a final salt concentration of 200 mM NaCl before loading onto a 1 mL heparin column (GE Healthcare) equilibrated in low-salt buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... TAMRA fluorescence (542/568 nm excitation/emission) was detected using a Typhoon FLA 9500 instrument (GE Healthcare). Gels were then stained with Coomassie blue and destained ...
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bioRxiv - Biochemistry 2022Quote: ... or a Superose 6 prep grade XK 16/600 column (GE Healthcare). Columns were equilibrated in Ndc80 buffer (50 mM Hepes ...
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bioRxiv - Cell Biology 2022Quote: ... The formed BsAbs were preliminary processed by Protein A resin (GE Healthcare) and subsequently purified employing HiTrap Capto MMC ImpRes (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred (100 V, 1 hr) onto PVDF (polyvinylidene difluoride) membranes (GE Healthcare Life Sciences) using 1X Tris/Glycine buffer (in-house 50X solution containing 250 mM Tris and 1920 mM Glycine) ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were detected using enhanced chemiluminescence reagents (Amersham ECL Prime Western Blotting detection) in an Amersham Imager 600 analyzer or visualized by ECL (GE Healthcare, UK). ...
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bioRxiv - Cell Biology 2022Quote: ... was then loaded to a 5 mL HisTrap Ni-NTA column coupled to AKTA Pure protein purification system (GE Healthcare). The His-SUMO-tagged CAP1 protein was eluted from the nickel column with an imidazole gradient using buffer A and buffer B (buffer A + 250 mM imidazole) ...
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bioRxiv - Cell Biology 2022Quote: ... the clarified extract was loaded on a Ni Sepharose high performance column (GE Healthcare Life Sciences, ref 17526802). Resin was washed with Wash buffer (20 mM Tris pH8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection was performed with ECL Select Reagent (GE Healthcare, Cytiva) using UVITec Alliance LD2 (UVITec Cambridge ...
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bioRxiv - Microbiology 2022Quote: ... Radioactive signal was visualized by exposing 4 h a Storage Phosphor Screen BAS-IP MS 2040 (Fujifilm) then imaged with a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... PBMCs were isolated by Ficoll-Paque (GE Healthcare) density gradient centrifugation and cryopreserved in 90% FBS + 10% DMSO (v/v) ...
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bioRxiv - Plant Biology 2022Quote: ... coli using Glutathione Sepharose beads (GE healthcare). The GST-tagged protein-bead complexes were incubated with 2 μg of MBP-tagged proteins in 1 mL of pull-down buffer (50 mM Tris ...
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bioRxiv - Plant Biology 2022Quote: ... Autoradiograph images were obtained by Typhoon Trio imager (GE healthcare).
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bioRxiv - Biochemistry 2022Quote: ... concentrated and purified on a Superdex 200 16/600 column (GE Healthcare Life Sciences) in Buffer C ...
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bioRxiv - Biochemistry 2022Quote: ... supplemented with 10% fetal calf serum (GE Healthcare) in a 37°C incubator at 5% CO2 for 48 hours before the experiment ...
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bioRxiv - Biochemistry 2022Quote: ... were subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column connected to an AKTA PURE 25 FPLC system (GE Healthcare Life Sciences) maintained at 6° C ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Biochemistry 2022Quote: ... The dissolved ammonium sulfate pellet was loaded on a 5 mL DEAE Sepharose and 5 ml Ni-Sepharose cartridge (GE Healthcare Life Sciences) connected in tandem ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were subsequently transferred to Protran 0.2 μm nitrocellulose membrane (GE Healthcare, Chicago, USA). Efficiency of protein transfer was checked by Ponceau staining ...
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bioRxiv - Biochemistry 2022Quote: Recombinant CrRPE1 was injected on a Superose 6 Increase 10/300 GL column (GE Healthcare, Chicago, Illinois) and isocratically eluted in 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The treated sample was further purified by IMAC and was subjected to size-exclusion chromatography (SEC) using HiLoad Superdex 200 pg (GE Healthcare Life Sciences) in 10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease as described previously32 ...
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bioRxiv - Biochemistry 2022Quote: ... The biotinylated Norrin samples were immobilized onto the surface of a CM5 sensor chip (GE Healthcare Life Sciences) on which approximately 8500 resonance units of streptavidin were coupled via primary amines ...
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bioRxiv - Biochemistry 2022Quote: SPR experiments were performed using a Biacore T200 machine (GE Healthcare Life Sciences) at 25°C in 10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... where B is the amount of bound analyte and C is the concentration of analyte in the sample) for the calculation of dissociation constant (Kd) values using Biacore Evaluation software (GE Healthcare Life Sciences). Data points correspond to the average from two independent dilution series.
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bioRxiv - Biochemistry 2022Quote: ... as previously described.[58] Desalting was carried out using a PD-10 column (GE Healthcare Life Sciences, UK). For PCMjMAT the protein was further purified by heating at 80 °C for 20 min followed by 30 min centrifugation at 18213 × g (Eppendorf centrifuge 5427 R) ...
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bioRxiv - Biochemistry 2022Quote: ... The complementary DNA of human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare. The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... Pol η was loaded onto a HisTrap HP column (GE Healthcare), which was pre-equilibrated with a buffer that contained 20 mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... Pol η was cleaned with a Superdex 200 10/300 GL column (GE Healthcare) with a buffer that contained 20 mM Tris (pH 7.5) ...