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Citations for GE Life Sciences :
9601 - 9650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and quantified with ImageQuantTL software (GE Healthcare Life Science).
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bioRxiv - Biochemistry 2022Quote: ... RSA-sulfo-MBS was desalted on a PD10 column (GE Healthcare) with PBS at pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... and ran through an S75 size exclusion column (GE healthcare) in the NMR buffer (20 mM KPO4 pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... filtered with a 0.2µm filter and loaded into a Q column (GE healthcare) to remove suspected lingering proteases and DnaK ...
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bioRxiv - Bioengineering 2022Quote: ... followed by biotinylation and size-exclusion chromatography with a Superdex S75 column (GE Healthcare). In general ...
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bioRxiv - Bioengineering 2022Quote: ... FPLC-purified biotinylated proteins (ligands) in HBS-P+ Buffer (GE Healthcare) were captured on a Streptavidin (SA ...
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bioRxiv - Bioengineering 2022Quote: ... All proteins were further purified by size-exclusion chromatography using an S75 or a S200 Increase column (GE Healthcare), depending on protein size ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by Illustra GFX PCR DNA and a gel band purification kit (GE Healthcare). DNA sequencing was accomplished using 3130xl Genetic Analyzer (Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... Afterwards the lysates were subjected to SDS-polyacrylamide gel electrophoresis using an 8% polyacrylamide gel and then electroblotted onto a nitrocellulose membrane with a pore size of 0.45 μm (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... before further purification with size exclusion (HiLoad 26/600 Superdex 200 pg., GE Healthcare) into the high salt buffer (100 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Excess calcein was removed with a desalting column (PD-10, GE Healthcare) that had been equilibrated with the dialysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Elutions were then run over a heparin column (HiTrap Heparin HP, GE Healthcare), washed with 47.5 mM NaCl buffer and eluted with a high salt buffer (100 mM Tris Base pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions with CasDinG were pooled and desalted (HiPrep 26/10 Desalting, GE Healthcare) into low salt buffer (100 mM Tris Base pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare) pre-equilibrated with 100 mM HEPES (pH 7.0) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Biochemistry 2022Quote: ... Gradients were then centrifuged at 31,000g for 16 hours in a Beckman SW32Ti rotor and fractionated using a Brandel fractionator apparatus and an AKTA Prime FPLC system (GE Healthcare). The profile was monitored by UV absorbance at A254 and the relevant fractions were collected ...
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bioRxiv - Biochemistry 2022Quote: ... The column was calibrated using the High Mr gel filtration calibration kit (GE Healthcare) and plotted with linear regression via GraphPad Prism v8.0.1 to resolve MtDPP Mr (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... and passed over an S-400 gel filtration column (GE Healthcare) with 50 mM HEPES-KOH (pH 7.9) ...
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bioRxiv - Biochemistry 2022Quote: ... except that a HiTrapTM Q HP column (GE Healthcare #29-0513-25) was used ...
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bioRxiv - Biochemistry 2022Quote: ... and passed through two subsequent gel filtration steps using a: 1) Hiload 16/60 Superdex 75 column (Cytiva #28989333) and 2) and Sephacryl S-300 column (GE Healthcare). Buffers used for this sizing step and subsequent labeling reactions were followed as for full-length proteins ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was loaded onto HisTrapTM FF crude column (GE Healthcare #17-5286-01) and then washed with 40 CV of resuspension buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Further purification was performed in column buffer (25 mM Tris HCl pH 8.0 and 150 mM NaCl) using HiLoad Superdex 200 gel-filtration chromatography (GE Healthcare). The peak fractions were collected ...
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bioRxiv - Biochemistry 2022Quote: ... cleaved protein was further purified by gel filtration using a Sephacryl S-400 column (GE Healthcare) with one of two buffers ...
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bioRxiv - Biochemistry 2022Quote: ... was separated on a denaturing formaldehyde/formamide 1.2 % agarose gel and transferred and UV-crosslinked onto Amersham Hybond™-N membrane (GE Healthcare). RNA was visualized using [32P]-radiolabeled probes targeting mitochondrial RNAs as indicated (see Supplementary Table S1).
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5) using an ÄKTA Purifier system (GE Healthcare). Fractions containing the desired protein were pooled and concentrated to a volume of 5 mL ...
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bioRxiv - Biochemistry 2022Quote: ... Separated protein was then transferred onto nitrocellulose membrane (GE healthcare) or ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was added to a 5 mL Ni-Sepharose fast-flow resin (GE Healthcare Life Sciences) pre-equilibrated with buffer A at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved sample was purified by a 5-mL HiTrap Heparin column (GE Healthcare), and polished using a HiLoad 16/60 Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and polished using a HiLoad 16/60 Superdex 200 column (GE Healthcare) equilibrated in 25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... and analysed using a phosphoimager (Typhoon FLA 9500; GE Healthcare). In vitro kinase assays probed by immunoblot analysis was carried out similarly as described above ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were imaged using a TyphoonTM FLA 9500 biomolecular imager (GE Healthcare) using recommended filter and detector settings based on the fluorophore being used and analysed with ImageJ (Schneider et al. ...
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bioRxiv - Biochemistry 2022Quote: Verification of the monomeric or dimeric state of those constructs was obtained by SEC-MALS experiments with the injection of 5 mg/mL of each protein into a Superdex 200 10/30 Increase column (GE Life Sciences, Velizy-Villacoublay, France) mounted on a HPLC Ultimate 3000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was first purified by Immobilized Metal Affinity Chromatography (IMAC) by loading the clarified lysate onto a 5 mL Nickel column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was loaded onto a Sephacryl-200 gel-filtration column (GE Healthcare) that was pre-equilibrated with the dialysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were transferred onto nitrocellulose membranes (GE Healthcare, Illinois, USA) using a Trans Blot turbo apparatus (BioRad ...
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bioRxiv - Biochemistry 2022Quote: ... The substrate was purified on a Mono Q 5/50 GL column (GE Healthcare) in buffer 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... VRC293 cells were expanded to grow in suspension in serum-free Expi293 media supplemented with 4 mM L-glutamine through a total of 8 passages via 1.0 L and 3.0 L shake flasks and 25 L Wave bioreactor (GE Healthcare) into a Biostat Cultibag STR 200 Plus (Sartorius Stedim Biotech ...
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bioRxiv - Biochemistry 2022Quote: ... SEC-HPLC was carried out using an Alliance e2695 HPLC system (Waters Co., Milford, MA, USA) equipped with a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and a 2998 photodiode array (PDA ...
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bioRxiv - Biochemistry 2022Quote: Size exclusion gel filtration chromatography was conducted with an AKTA FPLC system (GE Healthcare Life Sciences, Pittsburg, PA) using a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... Gels were dried onto Amersham Hybond-XL (GE Healthcare) and imaged using a phosphor screen ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and applied onto HisTrap 5 mL (GE Healthcare, USA) using an imidazole gradient from 25 – 250 mM ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and applied onto GSTrap FF 5 mL (GE Healthcare, USA) using a buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... size-exclusion column (SEC) using HiLoad 26/600 Superdex 200 (GE Healthcare, USA) was performed to separate thrombin and the GST-tag cleaved protein in a SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by size exclusion column (SEC) using HiLoad 26/600 Superdex 200 (GE Healthcare, USA) in SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: ... NC membrane was obtained from GE Healthcare Life Science(Germany) ...
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bioRxiv - Bioengineering 2022Quote: ... all proteins were then further purified by size exclusion chromatography using a Superdex 75 10/300 GL increase column (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Size exclusion chromatography on a Superdex 200HR (10/100 GL) column (GE Healthcare) in HEPES-NaOH buffer (20 mM ...
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bioRxiv - Biochemistry 2022Quote: ... Reaction mixtures were applied to a Superose 6 increase 10/300 column (GE Healthcare) equilibrated in 20 mM TRIS pH 8.0 ...