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Citations for GE Life Sciences :
9251 - 9300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions were combined and further purified by size exclusion chromatography on a Superdex S75 prep grade column (GE Healthcare Life Science) in sodium phosphate–EDTA buffer (10 mM sodium phosphate pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... the cell lysate was incubated with Ni-NTA beads (Ni Sepharose 6 Fast flow, GE Healthcare) for 45 mins ...
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bioRxiv - Microbiology 2022Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Antibodies were analyzed by size exclusion chromatography (GE Healthcare) and monomeric fractions were isolated in case of aggregation levels >5% ...
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bioRxiv - Microbiology 2022Quote: ... IgG1 and IgG2 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... siRNA (Catalog no. L-013607-00-0005) Human DHX9 (1660) siRNA (Catalog no. L-009950-00-0005) were from Ge Healthcare Dharmacon (Colorado ...
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bioRxiv - Microbiology 2022Quote: ... following manufacturer’s instruction (GE Healthcare Dharmacon Inc., Colorado, USA). 12 hours post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... pore size 0.20 µm (GE Healthcare). Membranes were blocked with Odyssey Blocking Buffer (Li-COR ...
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bioRxiv - Cell Biology 2022Quote: Super-resolution imaging of fixed cell samples with 3D-SIM was performed on a DeltaVision OMX V3 system (Applied Precision Imaging/GE Healthcare) equipped with a 100x/1.40 NA PlanApo oil immersion objective (Olympus) ...
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bioRxiv - Cell Biology 2022Quote: Super-resolution imaging of fixed cell samples with 3D-SIM was performed on a DeltaVision OMX V3 system (Applied Precision Imaging/GE Healthcare) equipped with a 100x/1.40 NA PlanApo oil immersion objective (Olympus) ...
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration is adjusted and protein is loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES- KOH pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to a 0.2 μm nitrocellulose membrane (GE Healthcare) by wet transfer in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The untagged Cas9 was concentrated and applied to a Superdex 200 16/600 (GE Healthcare) and eluted with 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Chemiluminescence was detected using ImageQuant LAS 4000 mini digital imaging system (GE Healthcare) and membranes were exposed with 1 min increment for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... and 0.2 µm Amersham Hybond sequencing PVDF membrane (GE Healthcare) were used ...
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bioRxiv - Microbiology 2022Quote: ... Abcam prestained mid-range protein ladder (Ab116027) and 0.45 µM Amersham Hybond PVDF membrane (GE Healthcare) were used ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then centrifuged at 9,000 g for 10 min and supernatant was collected and mixed gently with 200 µl of Ni Sepharose High Performance (GE Healthcare) and incubated for 1 h in same condition as above ...
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bioRxiv - Cell Biology 2022Quote: ... The raw data was then computationally reconstructed using Wiener filter settings 0.002 and channel-specifically measured optical transfer functions (OTFs) using the softWoRX 6.0 software package (Applied Precision) to obtain a super-resolution 3D image stack with a lateral (xy ...
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bioRxiv - Cell Biology 2022Quote: ... The luminescence signal was read on an Amersham Imager680 (GE Healthcare, North Richland Hills, TX, USA). The relative levels of proteins were quantified by scanning densitometry using the ImageJ™ program v1.48 (National Institutes of Health ...
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bioRxiv - Biophysics 2022Quote: ... bacteria were harvested and proteins were purified on a Ni-NTA resin (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... the eluted protein buffer was exchanged with phosphate buffered saline (PBS, Biological Industries, Reference number: 02-023-1A) using a desalting column (Sephadex G25, GE Healthcare). Protein labeling levels were determined by absorption at 450 nm ...
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bioRxiv - Biophysics 2022Quote: ... and loaded onto a HiTrap Heparin HP 1ml column (GE healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2022Quote: ... was filtered using 0.22 μM syringe filter and loaded onto a HiTrap Heparin HP 1ml column (GE healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Genomics 2022Quote: ... Rolling circle amplification was then performed using the Illustra TempliPhi 100 Amplification Kit (GE Healthcare). Precipitated DNA was resuspended directly in 20 µl of the Illustra TempliPhi sample buffer and the amplification reaction was allowed to proceed for 24 hours at 30°C.
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bioRxiv - Cell Biology 2022Quote: ... Pull down experiments were performed with Glutathione-Sepharose beads (GE Healthcare) coated with 0.5 μg of the indicated GST-tagged proteins and 2 nM of eluted 6xHis-kazrin C incubated in 1 ml of binding buffer containing PBS or 2 nM of the dynactin complex in 0.5 ml of DBB (25 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... Detection of proteins was performed using the ECL kit (GE Healthcare).
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bioRxiv - Physiology 2022Quote: We used a 3.0 Tesla MR scanner (SIGNA Premier, GE Healthcare, Milwaukee, USA) to obtain the MR scans ...
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An apical protein, Pcr2, is required for persistent movement by the human parasite Toxoplasma gondiibioRxiv - Cell Biology 2022Quote: ... placed onto the heated stage in an OMX-Flex imaging system (Applied Precision Inc.) and imaged with a heated 60X silicone oil immersion lens (NA 1.3 ...
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An apical protein, Pcr2, is required for persistent movement by the human parasite Toxoplasma gondiibioRxiv - Cell Biology 2022Quote: ... image stacks were acquired at 37°C using a DeltaVision imaging station (Applied Precision) with a COOLSNAPHQ2 camera (Photometrics®) ...
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An apical protein, Pcr2, is required for persistent movement by the human parasite Toxoplasma gondiibioRxiv - Cell Biology 2022Quote: ... Deconvolution of the image stacks was carried out using Softworx (Applied Precision). Images were contrast adjusted to optimize their display.
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bioRxiv - Plant Biology 2022Quote: ... The result was visualized by chemiluminescence using the ECL Prime Western blotting reagent (GE Healthcare) and a Fusion Solo S6 (Vilber-Lourmat ...
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bioRxiv - Biophysics 2022Quote: The N-terminal 6x His tags were removed from the histones by thrombin protease (GE Healthcare) treatments using 1 unit per 1 mg of protein for 3-5h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... and followed by SP sepharose column chromatography (GE Healthcare). Purity of purified HMG doman of Sox6 protein was analyzed by using 18% SDS-PAGE and stained with coommassie blue.
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bioRxiv - Molecular Biology 2022Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hours at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl and 1 mM DTT (survivin SEC buffer) and gel filtration using a Superdex 75 100/300GL column (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... The probes (PCR amplified from gDNA or binary plasmid using the oligos in Supplemental Table S3) were internally labelled using [α-P32] dCTP (BRIT India) using Rediprime labelling kit (GE healthcare) and hybridisation and washes were performed as described ...
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bioRxiv - Molecular Biology 2022Quote: ... then transferred to Hybond-NX membrane (GE Healthcare) in 0,5× TBE buffer for 1h at 350 mA of current and EDC-cross-linked for 45 min at 60°C with a solution containing 33 mg/ml of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Genomics 2022Quote: ... ribosome complexes were enriched using MicroSpin S-400 HR Columns (GE Healthcare) and RNA extracted using the Direct-zol RNA Miniprep Kit (Zymo Research) ...
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bioRxiv - Biophysics 2022Quote: ... with salt gradient of (250-1000mM NaCl for PARP1 and 50mM-1000mM NaCl for all other constructs) and finally through gel filtration (16/600 200pg or 16/600 75pg, GE Healthcare, depending on the size of the protein) column pre-equilibrated with buffer 150mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... after which the reaction mixture was passed through the salt exchange column (HiPrep 16/10 Desalting, GE Healthcare) with FPLC UV filter set at 254nm and then concentrated using 10kDa concentrator ...
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bioRxiv - Biophysics 2022Quote: ... followed by ion exchange Heparin column (HiTrap Heparin HP, GE Healthcare) with salt gradient of (250-1000mM NaCl for PARP1 and 50mM-1000mM NaCl for all other constructs ...
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bioRxiv - Biophysics 2022Quote: ... supernatant was passed through Ni-NTA affinity column (5ml, HisTrap column, GE Healthcare), followed by ion exchange Heparin column (HiTrap Heparin HP ...
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bioRxiv - Biophysics 2022Quote: ... Obtained PAR was diluted with 20mM Tris-HCl buffer pH 7.5 and was loaded into Resource Q anion exchange 1ml column (GE Healthcare) for fractionation with a linear gradient of 0 - 1M NaCl at 0.25ml/min flow rate ...
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bioRxiv - Cell Biology 2022Quote: ... followed by transfer by electroblotting to nitrocellulose membrane (GE Healthcare, Chicago, IL, USA). TSPP1 was detected using a rabbit crude antiserum (dilution 1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied on a 1.0 ml GSTrap 4B column (GE healthcare), the column was washed with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting total RNA was desalted by passing through MicroSpin G-50 column (GE Healthcare, Cat. #27533001) and the tRNA was deacylated through β-elimination in 50 mM Tris–HCl (pH 9.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... The blots were exposed to phosphor imaging screen and scanned using Typhoon scanner (GE healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... exposed to phosphor imaging screen and scanned using Typhoon scanner (GE healthcare). Post-scanning ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was electroblotted onto Hybond N+ membrane (GE healthcare) and UV crosslinked ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding 50 mM sodium succinate (pH 5.0) followed by buffer replacement by using Column PD 10 desalting column (GE Healthcare). Antibody SN-38 drug conjugates were prepared using purified antibody [30] ...