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Citations for GE Life Sciences :
9551 - 9600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The GST-tagged constructs for the single-molecule studies were purified using glutathione Sepharose (GE Healthcare), and eluted by TEV cleavage.
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bioRxiv - Cancer Biology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation using Ficoll-Paque Premium (#17-5442-02; GE Healthcare). PBMCs were washed 3x with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were further purified using a HiTrap Q HP cation exchange column (GE Healthcare). Further details are available in (Khan et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Sonicated cell lysate was loaded into a 5 ml HisTrap HP column (GE Healthcare) and purified by nickel affinity chromatography ...
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bioRxiv - Biophysics 2022Quote: ... This was followed by size exclusion chromatography (SEC) using 24 ml Superdex 200 Increase 10/300 GL column on an AKTA-FPLC system (GE Healthcare). Analytical gel filtration was performed in 20 mM Tris pH 7.5 and 150 mM NaCl at the flow rate of 0.5 ml/min.
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bioRxiv - Biophysics 2022Quote: ... The concentrated protein was filtered through a 0.2 µm filter and injected on a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... A Resource S (GE Healthcare) cation exchange column was equilibrated with resuspension buffer with 1% n-octyl-β-D-glucoside and the sample was injected and washed with the same buffer ...
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bioRxiv - Biophysics 2022Quote: ... All the data were collected in SEC-SAXS mode with an in-line Superdex 200 Increase 10/300 GL column (GE Healthcare). Both proteins were concentrated to 8 mg/mL and centrifuged at 20,000 x g immediately before injecting the protein onto the column ...
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bioRxiv - Biophysics 2022Quote: ... Isolated dimeric fractions of NQO1 variants were exchanged to HEPES-KOH buffer 50 mM pH 7.4 using PD-10 columns (GE Healthcare). The UV–visible spectra of purified NQO1 proteins were measured in a Cary spectrophotometer (Agilent Technologies ...
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bioRxiv - Biophysics 2022Quote: ... resolved in polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate (SDS-PAGE, 12% acrylamide) gels and transferred to PVDF membranes (GE Healthcare, Chicago, IL, USA) using standard procedures ...
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bioRxiv - Biophysics 2022Quote: ... this affinity chromatography step was carried out in an AKTA pure System (GE Healthcare) with a 5 mL Histrap® Excel column ...
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bioRxiv - Biophysics 2022Quote: Cy3B-NHS ester (PA63101) was purchased from GE Healthcare Life Sciences (Pittsburgh ...
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bioRxiv - Cancer Biology 2022Quote: ... according to manufacturer’s instructions (GE Healthcare). Purified mononuclear cells were suspended in PBS with supplemented with 10 μg/ml mouse gamma globulin (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2022Quote: ... according to manufacturer’s instructions (GE Healthcare). For serial transplantation ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer plus 0.03% digitonin ...
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bioRxiv - Biophysics 2022Quote: ... The sample was loaded onto an immobilized metal-affinity column (1 mL, HIStrap HP column, GE Healthcare), which was washed with 50 mM sodium phosphate (NaPi ...
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bioRxiv - Biophysics 2022Quote: ... 32 and purified according to an established protocol 32 with the exception that the Mono Q® ion-exchange purification step was exchanged with a size exclusion chromatography step using a Superose6 10/300 GL column (GE Healthcare) pre-equilibrated with PBS buffer 33 supplemented with 2 mM DTT ...
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bioRxiv - Biophysics 2022Quote: ... All proteins were further purified on a 5 ml HiTrap Heparin HP column (GE Healthcare), equilibrated in their respective dialysis buffer and eluted by an NaCl gradient containing 1 – 1.5 M NaCl ...
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bioRxiv - Biophysics 2022Quote: ... were additionally purified on a size exclusion chromatography column (Superdex 200 Increase column (GE Healthcare)) equilibrated with buffer E (50 mM KPi ...
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bioRxiv - Biophysics 2022Quote: ... All different DinB variants were purified using a 5 ml Ni2+-Histrap HP column (GE Healthcare) column equilibrated with buffer A and subsequently eluted with buffer B (buffer A supplemented with 1 M imidazole) ...
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bioRxiv - Biophysics 2022Quote: ... excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... NQO1 proteins were purified using immobilized nickel affinity chromatography columns (IMAC, GE Healthcare) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates or mitochondria samples were separated on 10 – 18 % Tris-Tricine gels and transferred onto Amersham™ Protran™ 0.2 µM nitrocellulose membranes (NC, GE Healthcare). For immunodetection ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were incubated for 2 h at room temperature and visualized on X-ray films using enhanced chemiluminescence detection kit (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Radioactive labeled mitochondrial translation products were detected using Phosphor screens and Amersham Typhoon imaging system (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... Samples were blotted onto a Hybond membrane (GE Healthcare) in 20X SSC ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA samples were loaded onto an Amersham Hybond-N+ membrane (RPN119B, GE Healthcare) using a Bio-Dot apparatus (#170-6545 ...
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bioRxiv - Cancer Biology 2022Quote: ... the sample was concentrated and then buffer exchanged using a Nap10 (GE healthcare) column equilibrated in buffer B (20 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: Images of mitotic cells and metaphase spreads were acquired on Deltavision Elite imaging system (GE Healthcare) equipped with a Cool SnapHQ2 charge-couple device (CCD ...
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bioRxiv - Cancer Biology 2022Quote: ... and optical sections were collected at 200-nm intervals and processed using ratio deconvolution in softWoRx (GE Healthcare, version 6.5.2). Images were prepared for publication using FIJI (Version 2.1.0/1.53o) ...
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bioRxiv - Cancer Biology 2022Quote: ... phospho-p38 MAPK (Invitrogen, #36-8500, 1:1000. Chemiluminescence was recorded using the Image Quant Las4000 imaging system (GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... Bound antibodies were detected using chemiluminescence (ECL, GE Healthcare, Cat# GERPN2232) and imaged using the Fusion Fx Imaging System (Vilber ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR product purification was done with streptavidin-sepharose high-performance beads (GE Healthcare Life Sciences, Piscataway, NJ), and co-denaturation of the biotinylated PCR products and sequencing primer (3.6 pmol/reaction) ...
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bioRxiv - Cancer Biology 2022Quote: ... Sepharose CL-4B was purchased from GE Healthcare Bio-Sciences (Mississauga ...
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bioRxiv - Cancer Biology 2022Quote: Fluorescence deconvolved images were taken using the DeltaVision Microscope system (GE Healthcare Life Sciences). EVs were observed using glass bottom chamber slides (80807 ...
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bioRxiv - Biochemistry 2022Quote: ... Chemiluminescence detection was performed using ECL Select Peroxide and Luminol solutions (GE Healthcare) according to the manufacturer’s directions.
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were concentrated and further purified by size exclusion chromatography (SEC) on a Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were incubated with immunoglobulin G (IgG) Sepharose 6 Fast Flow beads (GE Healthcare) on a rotating wheel at 4 °C for 90 min ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by consecutive centrifugation at 5,000 and 18,000 rpm and incubated with IgG Sepharose 6 Fast Flow beads (GE Healthcare; Cat# 17096902) on a rotating wheel for 120 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Pre-ribosomal particles were purified via immunoglobulin G (IgG) Sepharose 6 Fast Flow beads (GE Healthcare) as described above ...
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bioRxiv - Biochemistry 2022Quote: ... The gel was incubated with phosphor imaging plate (GE healthcare), scanned using Typhoon FLA 9500 (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... 45 μl of the protein at 4.43 mg ml−1 (70.4 µM) was injected in SEC column S200i 5/150 (GE Life Sciences, Velizy-Villacoublay, France) with a buffer containing 20mM Hepes pH 7,5 ...
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bioRxiv - Biochemistry 2022Quote: ... Mobicol Classic columns (MoBiTec, GmbH) were assembled and 50 μl of streptavidin Sepharose (GE Healthcare) was transferred to each column ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... scanned using Typhoon FLA 9500 (GE Healthcare) and analysed using ImageJ.
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bioRxiv - Biochemistry 2022Quote: ... The target proteins were purified with HisTrap HP columns (GE Life Sciences, Velizy-Villacoublay, France). Further purification was achieved by size-exclusion chromatography in buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... The PreScission-treated Mpro was applied to connected GSTtrap FF (GE Healthcare) and nickel columns to remove the GST-tagged PreScission protease ...
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bioRxiv - Biochemistry 2022Quote: Samples of the different complexes were prepared at 10 μM and 100 μl were injected onto a 2.4 mL Superdex 200 Increase gel filtration column (GE Healthcare) pre-equilibrated in 100 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The gel was imaged with a Typhoon Imager (GE Healthcare). All experiments were repeated three or more times in independent replicates.
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bioRxiv - Biochemistry 2022Quote: ... and loaded onto a HisTrap HP column (GE Healthcare). The column was washed with eight column volumes of buffer A1 containing 75 mM imidazole and six column volumes of buffer A1 containing 100 mM imidazole ...