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Citations for GE Life Sciences :
551 - 600 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... followed by protein transfer onto an Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The membrane was blocked in 5% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were subsequently transferred to Protran 0.2 μm nitrocellulose membrane (GE Healthcare, Chicago, USA). Efficiency of protein transfer was checked by Ponceau staining ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred onto nitrocellulose membrane (Amersham Protran 0.2 μm nitrocellulose, GE Healthcare Lifescience) using a semi-dry transfer unit (TE77 ...
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bioRxiv - Developmental Biology 2022Quote: ... Separated proteins were electrophoretically transferred to polyvinylidene difluoride membranes (10600023, GE Healthcare, Milwaukee, WI) for 1 h at 100 V on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto nitrocellulose membrane (Amersham Protran 0.2 μm nitrocellulose, GE Healthcare Lifescience) using a semi-dry transfer unit (TE77 ...
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bioRxiv - Immunology 2023Quote: ... Proteins were transferred onto an AmershamTM ProtranTM 0.45 µm nitrocellulose membrane (GE Healthcare, UK) covered in 4+4 Whatman papers (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were subsequently electrotransferred onto a polyvinylidene difluoride membrane (PVDF, GE Healthcare, Poland) in a chilled transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred onto a nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA) by wet blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then transferred onto a nitrocellulose blotting membrane (10600003, GE Healthcare Life Science).
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bioRxiv - Microbiology 2023Quote: ... GP63 and α-Tubulin was performed by transferring proteins to nitrocellulose membranes (GE healthcare). The membranes were blocked ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane (Amersham Protran 0.45 NC, GE HealthCare) and stained with Ponceau red ...
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bioRxiv - Biochemistry 2023Quote: The protein gel was then transferred onto a membrane (GE Healthcare Life Science UK) and the membrane blocked in 5% semi skimmed milk powder in PBS ...
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bioRxiv - Immunology 2024Quote: ... The proteins were transferred to Amersham Protran nitrocellulose blotting membrane (GE Healthcare Life Sciences) and further incubated with anti IRF7 (polyclonal antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto Amersham™ Hybond® P PVDF membranes (GE Healthcare, #10600023) and detected with the following antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed using BCA and proteins was separated on SDS-PAGE followed by transfer to PVDF membrane (GE Healthcare). Next ...
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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bioRxiv - Plant Biology 2019Quote: ... Purification tags were removed by incubation with 3C protease (10 μg/mg fusion protein) followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/60 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... All constructs used for protein expression and purification in this study were GST fusions expressed from pGEX-4T1 (GE Life Sciences). The native proteins without signal peptides were expressed in BL21(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... The GST and GST fusion protein extracts were mixed with glutathione Sepharose 4B beads (71024800-GE; GE Healthcare, Chicago, IL, USA) overnight at 4 °C and the mixtures were then washed three times with ice-cold PBS ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μg of each fusion protein were radioiodinated with 125I and Chloramine T hydrate and purified on a PD10 column (GE Healthcare), as described previously [38] ...
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bioRxiv - Plant Biology 2020Quote: ... Purification tags were then removed by incubation with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: Purified trypsinized post-fusion hMPV B2 F protein was subjected to size exclusion chromatography (S200, 16/300, GE Healthcare Life Sciences) in 50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The former vector encodes a glutathione S-transferase (GST) fusion protein that can be removed proteolytically with Prescission Protease (GE Healthcare) after affinity purification ...
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bioRxiv - Microbiology 2021Quote: GST alone and GST-tagged WWOX WW1 and WW2 domains fusion protein were expressed in BL-21 cells and subsequently purified and conjugated to glutathione (GSH) beads (GE HEALTHCARE). HEK293T cells were transfected with eVP40-WT ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts of GST or GST-fusion proteins were incubated for 0.5-1h with glutathione-coated sepharose beads (GE Healthcare, Freiburg, Germany) equilibrated in PBS (Boi1-Bni1 ...
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bioRxiv - Genomics 2021Quote: ... we used the Ascaris proteins cleaved from GST-fusions bound to Glutathione Sepharose 4B by treatment with PreScission protease (GE Healthcare). AsWAGO-2 peptides (YASRRMFELTKQSRDDYRA and RNGGQETSSSSSGEAGHLDI ...
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bioRxiv - Plant Biology 2022Quote: ... Purification tags were then removed by incubating with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... The PfPHB1-and PfPHB2-GST fusion proteins were purified via affinity chromatography from bacterial extracts using glutathione-sepharose (GE Healthcare, USA) using the soluble fractions of the lysed bacterial cultures ...
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Cell Biology 2023Quote: GST fusion protein was similarly purified using affinity chromatography by incubating bacterial cell lysate with glutathione-Sepharose 4B beads (#17075601; GE Healthcare) according to the manufacturer’s instructions and eluted from beads with elution buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Escherichia coli BL21 (GE Healthcare) was transformed with each GST-fusion protein construct and the expression of GST-fusion protein was induced by 0.1 mM isopropyl-β-D-thiogalactoside (IPTG ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His (1:5000, mouse; GE Healthcare) and anti-GST (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... using a His SpinTrap column (GE Healthcare). Resultant elution fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Microbiology 2023Quote: ... Anti-His antibody was from GE Healthcare; anti-PhoP antibody was elicited in rabbit (AlphaOmegaSciences) ...
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bioRxiv - Biophysics 2023Quote: ... followed by Hi-Trap Heparin (GE Healthcare) and Mono Q anion exchange chromatography columns (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... His GraviTrapTM gravity flow columns (GE Healthcare) were used for protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... His Mag Sepharose Ni beads (GE Healthcare) were used for the protein-protein interaction assay ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysed cells were centrifuged 20,000 xg for 30 minutes at 4C and soluble lysate was filtered prior to IMAC purification using 2×5mL His-Trap columns (GE Healthcare) affixed to an Akta Pure ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... (amino acids 2-241 fused to 6 x his) were expressed in BL21 cells and purified on glutathione-linked sepharose beads (GE healthcare) or Ni-NTA-Agarose (GE healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein fraction obtained was further subjected to Hi-Load Superdex 200 pg 16/600 size-exclusion chromatography column (GE Healthcare/Cytiva) equilibrated with buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Biochemistry 2020Quote: The supernatant fraction containing the rhGFAT2 protein (with or without tag) was subjected to purification using a Ni+2NTA affinity column (HisTrap HP 5 mL, GE Healthcare, USA). The column was equilibrated with 10 column volumes (CV ...
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bioRxiv - Plant Biology 2019Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Plant Biology 2021Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 mM EDTA) and electroblotted onto a nylon (N+) membrane (GE Healthcare RPN203B) at 20 V for 90 min at 4 °C in 0.5x TBE buffer ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated for 2–3 minutes with ECL detection reagent (Amersham GE Healthcare), and were then developed using a ChemiDoc MP Imaging System (Bio-Rad) ...