Labshake search
Citations for GE Life Sciences :
7601 - 7650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... membranes were probed with an ECL anti-rabbit IgG secondary antibody (1:10,000) from GE Healthcare Life Science and imaged using a ChemiDoc imaging system (BioRad) ...
-
bioRxiv - Bioengineering 2022Quote: The binding affinity of the corresponding Fab versions of the top six affinity matured antibodies as well as the parental clone to α-cobratoxin and Dp7 was determined using Surface Plasmon Resonance (SPR) (BIAcore T100, GE Healthcare). Antigen immobilization and affinity measurements were performed as previously described8 ...
-
bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
-
bioRxiv - Immunology 2022Quote: ... The response units were recorded at room temperature using BIAcore 8K Evaluation Software (v3.0.12.15655; GE Healthcare), and the resulting data were fitted to a 1:1 binding model using BIAcore 8K Evaluation Software (v3.0.12.15655 ...
-
bioRxiv - Cancer Biology 2022Quote: ... A 30-μl aliquot of glutathione–Sepharose 4B beads (GE Healthcare) was added and incubated for 3 hours at 4 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... Co-cultures were imaged over time using an INCell 6000 high-content imager (GE Healthcare Life Sciences), with a 488 nm and a 633 nm laser excitation to visualize tumor cells and T cells (stained by CellTracker Deep Red Dye ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated with horseradish peroxide (HRP)-conjugated) anti-rabbit IgG or anti-mouse IgG secondary antibodies (1:10,000, GE Healthcare Life Science) at room temperature and proteins were detected using Pierce ECL Western Blotting Substrate (34095 ...
-
bioRxiv - Biophysics 2022Quote: ... a minor glycosylated product (10% of the total) was removed by incubating with ConA slurry (GE Healthcare) for 4 hours at 4°C on a rotator ...
-
bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
-
bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
-
bioRxiv - Cell Biology 2022Quote: ... Dharmacon DharmaFECT 1 transfection reagent (GE Healthcare, Manchester, UK) was used following the manufacturer’s siRNA transfection protocol with slight modification ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% fetal bovine serum (FBS, GE Healthcare, SH30396.03) and 100U/ml penicillin-streptomycin (Caisson Labs ...
-
bioRxiv - Molecular Biology 2022Quote: ... then loaded onto a 1mL HiTrap heparin HP column (GE Healthcare) pre-equilibrated in low salt buffer and eluted with a linear gradient of 100mM to 1M KCl over 40 column volumes (CVs) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and transferred onto polyvinylidene difluoride membranes (PVDF; GE Healthcare Life Sciences). Membranes were incubated with specific first antibodies against STAT3 at a concentration of 0.069 μg/mL and phospho-STAT3 (pSTAT3 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the gel was washed with the wash buffer and the IF3-bound complexes were eluted by additional incubation of 2 h with the PreScission protease (GE Healthcare) (2 U/μl).
-
bioRxiv - Molecular Biology 2022Quote: ... and immune complexes were captured using Protein G Sepharose (GE Healthcare).
-
bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% Fetal Bovine Serum (GE Healthcare, Logan, UT) and 1% penicillin /streptomycin (Gibco ...
-
bioRxiv - Molecular Biology 2022Quote: ... dilution) using Amersham Imager 600 (GE Healthcare). For antibody-based western blotting ...
-
bioRxiv - Molecular Biology 2022Quote: ... The signal was visualized using Amersham Imager 600 (GE Healthcare).
-
bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2022Quote: ... and run through a size exclusion column (Superose 6 10/300, GE Healthcare). Fractions containing purified monomeric Tau were concentrated as before and buffer exchanged to PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... the supernatant of the lysate was filtered through a 0.22 µm membrane filter and loaded onto a Ni-NTA column (HiTrap, 5 ml, GE Healthcare) using a low imidazole buffer (Buffer C ...
-
bioRxiv - Molecular Biology 2022Quote: ... and run through a size exclusion column (Superdex200 16/600, GE Healthcare) using PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... and visualization with a Typhoon scanner (Trio, GE Healthcare) using the 532 nm laser and a 610 nm band-pass filter.
-
bioRxiv - Molecular Biology 2022Quote: ... the cleared lysate was applied to a 10 ml Strep-Tactin column (Iba) and a gel filtration column (Superdex200 16 600, GE Healthcare). The purified protein was stored in PBS containing 1 mM TCEP at -80°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT and applied to a prewetted nitrocellulose membrane (GE Healthcare, 0.2 µm) under vacuum using a Dot Blot Apparatus (SCIE-PLAS) ...
-
bioRxiv - Molecular Biology 2022Quote: ... run through a cation exchange column (HiTrap SP HP,5 ml, GE Healthcare), and eluted with a high salt buffer (Buffer B ...
-
bioRxiv - Molecular Biology 2022Quote: ... and visualization with a Typhoon scanner (Trio, GE Healthcare) using the 532 nm laser and a 610 nm band-pass filter.
-
bioRxiv - Molecular Biology 2022Quote: ... The RNA in the gel was transferred overnight to a nitrocellulose membrane with a positive charge (GE healthcare Amersham™ Hybond™ -N+) by capillary transfer using an assembly of Whatman® filter paper ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were imaged using a high-resolution microscope DeltaVision™ Ultra (GE Healthcare, Boston, USA).
-
bioRxiv - Molecular Biology 2022Quote: ... The washed membranes were wrapped into Saran plastic wrap and exposed on phosphor imaging screens overnight before being scanned by a Typhoon™ FLA9500 (GE Healthcare Life Sciences). Intensity of radioactive bands were quantified with ImageJ ...
-
bioRxiv - Molecular Biology 2022Quote: ... The gel was exposed with phosphor imaging screens for 5 minutes before being scanned with a Typhoon™ FLA9500 (GE Healthcare Life Sciences). The bands corresponding to the probes were cut out of the gel and conserved at -20°C for future work ...
-
bioRxiv - Molecular Biology 2022Quote: ... late segmented schizonts were enriched by centrifugation on a 60% Percoll (GE Healthcare) cushion ...
-
bioRxiv - Molecular Biology 2022Quote: ... sheep anti-mouse-IgG and anti-rat-IgG (all from GE Healthcare, Chicago, IL, USA). The antibodies were detected with Enhanced Chemiluminescence kit (Thermo Fischer Scientific).
-
bioRxiv - Molecular Biology 2022Quote: ... 5 mM Imidazole) followed by cleavage with PreScission protease (60 U/ml, GE Healthcare Life Sciences). The beads were applied to a column and the elution was collected and completed to 10 mL with P5 Buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... the elute was loaded onto Superdex 200 16/600 column (GE healthcare) equilibrated with 50mM HEPES pH 7.4 ...
-
bioRxiv - Molecular Biology 2022Quote: ... and GST (glutathione S-transferase) Sepharose beads (17075601, GE Life Sciences). Recombinant human HBP (2200-SE-050 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the membrane was submerged in the ECL Plus Western Blotting Detection Reagents (GE Healthcare) for 2 min and then subjected to the imaging system (UVITEC Cambridge ...
-
bioRxiv - Molecular Biology 2022Quote: ... Sera-MagTM SpeedBeads magnetic protein A/G particles (GE Life Sciences), and GST (glutathione S-transferase ...
-
bioRxiv - Molecular Biology 2022Quote: Protein samples were run on 5∼20% precast gradient SDS-PAGE (ATTO) or 4∼20% precast gradient gel (Shanghai WSHT Biotechnology Inc.) and then transferred to Hybond ECL membranes (GE Healthcare) followed by western blot analysis with the indicated antibodies ...
-
bioRxiv - Molecular Biology 2022Quote: ... A final purification step was carried out by size-exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare) column equilibrated with storage buffer (50 mM Tris pH 7.5 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The membrane was scanned using chemiluminescence settings on an Amersham Imager 600 (GE Healthcare).
-
bioRxiv - Molecular Biology 2022Quote: ... The lysate was clarified by centrifugation at 35000 g for 30 minutes at 4° C and the supernatant was passed through a 0.45 µm membrane filter before loading onto a pre-equilibrated Strep Trap HP column (GE Healthcare) equilibrated previously with Strep Trap buffer A (50 mM Tris pH 8.5 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The concentrated product was loaded onto a Superdex 200 increase 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
-
bioRxiv - Molecular Biology 2022Quote: ... MicroCT imaging at 45µ resolution using a GE eXplore Locus platform (GE Healthcare/EVS Corp., London, ON, Canada) allowed 3D reconstructions to be created and adaptive regions of interest 3 mm deep were made in the central non-meniscus covered portion of the tibial plateau subchondral bone plate ...
-
bioRxiv - Molecular Biology 2022Quote: ... The lysate was clarified by centrifugation at 35000 g for 30 minutes at 4° C and the supernatant was passed through a 0.45 µ membrane filter before loading onto a pre-equilibrated HiTrap immobilized metal ion affinity (IMAC) column (HiTrap IMAC FF, GE Healthcare). The column was washed extensively with wash buffer (20 mM Tris pH 7.9 ...
-
bioRxiv - Molecular Biology 2022Quote: ... loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare). After a thorough washing step with the resuspension buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein quality was assessed by SDS-PAGE and fractions containing RNase E were pooled and loaded onto a butyl sepharose column (GE Healthcare) equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
-
bioRxiv - Molecular Biology 2022Quote: ... and the proteins were transferred on 0.45μm nitrocellulose membrane (10600002 Amersham, GE Healthcare). The membrane was blocked with 5% BSA in PBS for one hour at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... The HiLoad 16/600 Superdex 75 column (GE Healthcare) was used to separate the monomeric fractions of Nb35 with running buffer containing 20 mM HEPES ...