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Citations for GE Life Sciences :
7451 - 7500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... tagging of the protein was visualised by running an SDS PAGE followed by scanning in a Typhoon scanner (GE Healthcare, Sweden).
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bioRxiv - Biochemistry 2022Quote: ... The tagless protein was further purified by anion exchange chromatography (Mono Q 10/100 GL, GE Healthcare) in 25 mM potassium phosphate (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: Using an ÄKTApurifier UPC 10 FPLC system (GE Healthcare), lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection ...
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bioRxiv - Biochemistry 2022Quote: ... lysates were injected (200 µL per injection) onto a Superose® 6 10/300GL column (GE Healthcare, UK) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrate was loaded onto a size-exclusion chromatography (SEC) column (Superose 6 Increase 10/30 GL, GE Healthcare), equilibrated in a buffer composed of 20 mM Tris-Cl (pH 8.5) ...
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bioRxiv - Biochemistry 2022Quote: ... the Glutathione Sepharose 4B resin (GE Healthcare) was incubated with 5% BSA for 5 minutes and washed 3 times with 500 μL GST pull-down buffer containing 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing complex I were concentrated using Amicon Ultra 4 columns with 100,000 molecular weight cutoff and loaded onto a Superose 6 Increase 3.2/300 size-exclusion column (GE Healthcare) connected to an Äkta Ettan system ...
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bioRxiv - Biochemistry 2022Quote: ... connected to an Äkta Purifier system (GE Healthcare). The column was equilibrated with IEX buffer 50 (30 mM Hepes/NaOH pH 7.8 ...
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bioRxiv - Biochemistry 2022Quote: ... using a PD-10 column (GE Healthcare). Mad1 and GST-Mps1 were mixed at a 5:1 ratio ...
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bioRxiv - Biochemistry 2022Quote: ... and then again on a Superdex 200 5/150 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... p52 was concentrated and loaded onto the gel filtration column (HiLoad 16/600 Superdex 200 pg, GE Healthcare) pre-equilibrated with 10 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... Flow-through fraction was applied to SP HP column (GE Healthcare). The column was washed with 40 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... and two rounds of cation-exchange Resource S (GE Healthcare) and Superdex 75 (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved Cdc20N was then passed over a HisTrap column (GE Healthcare) and two rounds of cation-exchange Resource S (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... 100 μl purified protein at approximately 3.0 mg/ml was auto-injected onto Superdex 75 or a Superdex 200 Increase 10/300 GL column (GE healthcare) and run at 0.5 ml/min ...
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bioRxiv - Biochemistry 2022Quote: ... first on a Superdex 200 15/300 column (GE Healthcare), and then again on a Superdex 200 5/150 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... gel filtration was usually done with a Superdex 75 10 300 GL column (GE Healthcare), to further increase the purity of the product and to buffer-exchange to 200 mM ammonium acetate (pH 7°C).
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bioRxiv - Biochemistry 2022Quote: ... proteins were transferred to nitrocellulose membrane (GE Healthcare, Chicago, US) and detection was performed using Cy-5 labelled anti-VHH antibody (Cy™5 AffiniPure Goat Anti-Alpaca IgG ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were purified using Glutathione Sepharose 4B (GE Healthcare) and Sephacryl S100 columns (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and 40mM iodoacetamide solution (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... The Ulp1 treated protein sample was loaded directly onto a prepacked GSTrap column (GE Healthcare). Protease 3C was added to the eluted GST-AAGAB:AP2α binary complex to cleave the GST tag ...
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bioRxiv - Biochemistry 2022Quote: ... and Source-Q (GE Healthcare) in tandem.
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bioRxiv - Biochemistry 2022Quote: ... A Superdex 200 Increase 10/300 (GE Healthcare) column was used to separate empty detergent micelles from the protein-detergent complexes ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was purified with the Ni-NTA Superflow affinity column (GE Healthcare) and Sephacryl S100 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The K48C mutant of ubiquitin was desalted (HiPrep 26/10 Desalting column, GE Healthcare), and mixed with BFA at a molar ratio of 1:10 in 20 mM pH 7.4 HEPES buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was purified with Glutathione Sepharose 4B column (GE Healthcare) and Sephacryl S100 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was purified with Ni-NTA Superflow affinity column (GE Healthcare) and Sephacryl S100 columns (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and pUbl were purified on Source-Q column (GE Healthcare), buffer-exchanged ...
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bioRxiv - Biochemistry 2022Quote: ... and Sephacryl S100 columns (GE Healthcare) in tandem.
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bioRxiv - Biochemistry 2022Quote: ... ∼100% D2O) was slowly passed through Superdex 200 Increase 10/300 column (GE Healthcare) to exchange protonated DM to partially deuterated d-DDM (tail 89 % and head 57% deuterated ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was diluted with lysis buffer before application to a 5ml HiTrap STREP column (GE Healthcare), washed with lysis buffer and eluted using buffer supplemented with 2 mM Desthiobiotin ...
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bioRxiv - Biochemistry 2022Quote: ... and Sephacryl S100 columns (GE Healthcare) in tandem.
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bioRxiv - Biochemistry 2022Quote: ... and applied to a Superdex200 10/300 size exclusion column (GE Healthcare) to purify the protein to homogeneity in 10mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and Sephacryl S100 columns (GE Healthcare) in tandem ...
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bioRxiv - Biochemistry 2022Quote: ... and Sephacryl S100 column (GE Healthcare) in tandem ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was passed through the HisPur resin to remove eGFP-His8 tag and TEV protease and further purified using Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) in SEC buffer containing 0.06% UDM /0.2% DM ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were pooled onto GST affinity resin (GE Healthcare), incubated for 2 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... SEC was carried using a Superdex 200 Increase 10/300 column (GE Healthcare), a 0.1 ml/min flow rate ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was applied to a 5ml HiTrap TALON crude column (GE Healthcare) washed first with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The eluate of HIC-EBA was subjected to affinity chromatography using Strep-Tactin® column (5 ml cartridge, GE Healthcare Life Science ...
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bioRxiv - Biochemistry 2022Quote: ... Reduction and alkylation steps were performed using 10 mM dithiothreitol (DTT -GE Healthcare) and 40mM iodoacetamide solution (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... and extracts were submitted to SDS-PAGE and blotted onto nitrocellulose membranes (GE Healthcare Life Sciences) with the loading of protein being confirmed by internal nuclei and cytoplasm fraction control blotting of Lamin A ...
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bioRxiv - Biochemistry 2022Quote: ... and gel filtered through a Superose 6 10/300 GL column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved GST was separated by anion exchange chromatography (HiTrap Q HP 5 mL (GE Healthcare)) ...
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bioRxiv - Biochemistry 2022Quote: ... coli was essentially performed as described 15 with the modification that 25 mM HEPES buffer was used as buffer component in all purification steps and the final gel filtration was performed on a Superdex 200 HiLoad 16/600 column (GE Healthcare) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein expression and purification was performed as described for the MTW1c and the Ni-NTA eluate was desalted using a PD10 column (GE Healthcare) in desalting buffer (50 mM HEPES pH 7.5 ...