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Citations for GE Life Sciences :
7501 - 7550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His- Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... For quantitative crosslinking experiments the eluate was further purified on a Superdex 200 HiLoad 16/60 column (GE Healthcare) in gelfiltration buffer (30 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... fractions containing pure protein sub-complexes were subjected to size-exclusion chromatography (Superose 6 increase 10/300, GE Healthcare) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... a minor glycosylated product (10% of the total) was removed by incubating with ConA slurry (GE Healthcare) for 4 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked peptides were enriched by size exclusion chromatography on a Superdex Peptide PC 3.2/30 column (GE Healthcare) using water/acetonitrile/TFA (77.4/22.5/0.1 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was loaded onto a 5 ml HisTrap FF column (GE Healthcare Life Sciences) washed and equilibrated in buffer A (50 mM Hepes pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... column with an ÄKTA™ Pure (GE Healthcare). The column outflow was fed into a DAWN® HELEOS™ II MALS detector (Wyatt Technology) ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was applied to an immobilized metal ion affinity chromatography column (HisTrap FF, 5 mL resin; GE Healthcare, Little Chalfont, UK). After washing with 25 mL of the 1 x T-buffer containing 25 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The affinity-purified protein was then subjected to StrepTrap HP column (GE Healthcare) to remove silkworm-derived proteins and eluted with buffer containing 2.5 mM desthiobiotin (DTB ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were loaded at 0.5 ml/min onto a Superdex™ 200 Increase 10/300 GL (GE Healthcare) column with an ÄKTA™ Pure (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Retromer was further purified by gel filtration on a Superdex S200 16/60 analytical column (GE Healthcare) into 10 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... The resultant library was injected onto a Superose 6 10/300 column (GE Healthcare) equilibrated in TSG buffer on an AKTA FPLC ...
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bioRxiv - Biochemistry 2022Quote: ... The precipitated pellet was resuspended in Buffer A and the solution subjected to Ni(II) immobilized affinity chromatography using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) with a gradient from 100% buffer A to 100% buffer B (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... and remaining urea removed with a PD10 desalting column (GE Healthcare). The eluted protein was concentrated and applied to a Superose 6 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The chromatography was performed on an ÄKTA Purifier system (GE Healthcare). proMbxA peaks resulting from the SEC were pooled separately and stored with 20% glycerol at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... Histidine was removed with a PD10 desalting column (GE Healthcare) and the protein eluted with IMAC buffer.
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bioRxiv - Biochemistry 2022Quote: ... The eluant was concentrated and passed through a Superose 6 Increase 10/300 gel filtration column (GE Healthcare). The fraction corresponding to intact V-ATPase was kept and flash frozen ...
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bioRxiv - Biochemistry 2022Quote: ... an Amersham Cy3 mono-reactive NHS ester dye pack (GE healthcare) was resuspended with labeling buffer and mixed with enough purified APE1 to yield a 100 μL solution of 10 mg/mL APE1 ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescently labeling of Pol β followed the same procedure using an Amersham Cy5 mono-reactive NHS ester dye pack (GE healthcare). Labeling efficiency was determined by calculating the molar ratio of dye-to-protein within the solution by measuring absorbance at 280 nm and 530 nm for APE1-Cy3 or 649 nm for Pol β-Cy5 ...
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bioRxiv - Biochemistry 2022Quote: ... to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare). A linear 0-75 mM histidine gradient with elution buffer was used to elute proMbxA ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was concentrated and applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in the same buffer.
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bioRxiv - Biochemistry 2022Quote: ... and remaining urea removed with PD10 desalting column (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The separated species were concentrated to 1.3 - 2.5 mg/ml and 200 µl of each were applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with SEC buffer and connected to a miniDAWN TREOS II triple-angle light scattering detector (Wyatt Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... Histidine was removed with a PD10 desalting column (GE Healthcare) at room temperature and the protein eluted with cold IMAC buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Refolded MbxA was loaded on a 5 ml Ni2+ loaded HiTrap IMAC HP column (GE Healthcare) and eluted with a linear 0-75 mM histidine gradient with elution buffer (50 mM Tris pH 7.8 ...
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bioRxiv - Immunology 2022Quote: ... and transferred onto a nitrocellulose membrane (GE Healthcare, 0.45 μm pore size of the membrane). Proteins were transferred to the membrane using 450 mA for 1.5 h ...
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bioRxiv - Systems Biology 2022Quote: ... Then the supernatant was collected and filtered by 0.2 μm Whatman Puradisc Polyethersulfone Syringe Filter (GE Healthcare), stored at -20 °C ...
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bioRxiv - Biophysics 2022Quote: ... they were incubated with Cy3 maleimide (GE Healthcare) or A488 C5 maleimide (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... using the illustra RNAspin Mini RNA Isolation Kit (GE Healthcare) following the “Protocol for total RNA purification from up to 109 bacterial cells,” which includes a 15-minute room-temperature incubation with RNase-free DNase I ...
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bioRxiv - Cell Biology 2022Quote: ... and developed using ECL Prime kit (GE Healthcare) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Bound antibodies were detected using peroxidase-labeled anti-rabbit IgG (GE Healthcare), or anti-rat IgG (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... Competition was studied using pH1HA10-Foldon immobilized on an NHS-EDC activated CM5 chip (GE HealthCare, Uppsala, Sweden) through amine coupling until 1500 RUs were achieved and subsequently probing with two analytes without an intermittent dissociation step ...
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bioRxiv - Immunology 2022Quote: ... proteins were separated on a Superose 6 Increase 10/300 column (GE Healthcare) in 1x PBS (pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... The soluble fraction was then allowed to bind to Ni-NTA resin (GE Healthcare Life Sciences, Amersham) at 25 °C and eluted in a gradient of imidazole (100 mM ...
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bioRxiv - Immunology 2022Quote: ... Leukocytes were collected at the interface of a 40%/80% Percoll gradient (GE Healthcare). Lymph nodes were mechanically disrupted for lymphocyte isolation ...
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bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... GCN4-M87 was subjected to gel filtration chromatography using a Superdex 200 (GE Healthcare) column equilibrated with HS buffer ...
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bioRxiv - Pathology 2022Quote: ... followed by electrophoresis and Western blotting on to PVDF membranes (Hybond P, GE Healthcare Bio Sciences, Pittsburgh, Pennsylvania). Primary antibodies against proteins of interest were compared to GAPDH as a housekeeping gene (see Supplemental Table 3) ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded onto a 5-mL glutathione column (GE Healthcare), followed by washing with 20 column volumes of buffer G plus 300 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were diluted with 2 volumes of buffer H and loaded onto a 1-mL SP HP column (GE Healthcare) and washed with buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... Total CD4+ T cells were collected from buffy coats by first performing a Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation to isolate peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Neuroscience 2022Quote: ... the retina was flat-mounted onto an Anodisc (#13, 0.2 µm pore size, GE Healthcare, Germany) with the ganglion cell layer (GCL ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by quantification using the ImageQuant software (GE Healthcare). The level of 47S pre-rRNA per lane was normalized to 28S rRNA.
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bioRxiv - Molecular Biology 2022Quote: ... and radioactive signal was acquired using a Typhoon 9500 scanner (GE Healthcare), followed by quantification using the ImageQuant software (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was loaded onto a Superdex s200 10/300 size exclusion column (GE Healthcare) and eluted in 100mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP, 5 mL; GE Healthcare) that had been previously equilibrated with Ni-NTA Buffer (NB ...
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bioRxiv - Biochemistry 2022Quote: All chromatography purification steps were carried out at 4°C using an ÄKTA pure fast protein liquid chromatography (FPLC) system (GE Healthcare). All buffer solutions were chilled prior to use ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated with HRP conjugated secondary antibody (IgG; mouse or rabbit) (GE Healthcare UK) at 1:5000 dilution in blocking buffer and incubated at RT with shaking for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were loaded on Hi-trap Q HP (GE Healthcare, cat.17115401) anion exchange column ...