Labshake search
Citations for GE Life Sciences :
701 - 750 of 1323 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... Streptavidin was coupled to CM5 sensor chips using an amino coupling kit (GE Healthcare Life Sciences) to near saturation ...
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bioRxiv - Immunology 2021Quote: ... Streptavidin was coupled to CM5 sensor chips using an amino coupling kit (GE Healthcare Life Sciences) to near saturation ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin was coupled to CM5 sensor chips using an amino coupling kit (GE Healthcare Life Sciences) to near saturation ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blots were visualized and quantified with enhanced chemiluminescence and imaging on a Fuji LAS 4 000 image station (GE Healthcare, Chicago, IL, US)4.
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad, Hercules, CA) for electrophoresis and transferred to a PVDF membrane (GE Healthcare Amersham, Chicago, IL). For cryba4 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Genomics 2021Quote: ... #S0282).[1] Reverse transcription was performed in 1.5 mL microfuge tubes under end-over-end rotation using a modified Reverse Transcription mix (1x Maxima H-RT buffer, 4% Ficoll PM-400 (GE Healthcare, #17-0300-05), 3 mM MgCl2 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Bioengineering 2020Quote: ... depending on machine availability and the type of imaging performed: LOGIQ Book XP with 8L-RS 4-12 MHz linear array probe (GE Healthcare, Milwaukee, WI, USA), Aixplorer with L14-5 probe (Supersonic Imagine ...
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bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Immunology 2024Quote: ... to 2 ml and subsequently loaded on a Sepharose 4 Fast Flow resin column (10 mm x 300 mm; GE Healthcare, Little Chalfont, UK), connected to the ÄKTA pure system (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... at a 1 to 20 ratio in Buffer A overnight at 4°C followed by a subtractive His trap purification using a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK) (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... were amine coupled on to a Series S CM5 sensor chip (GE Healthcare) using 2 adjacent flow cells (i.e ...
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bioRxiv - Microbiology 2020Quote: His-tagged 2019-nCoV S was immobilized to an NiNTA sensorchip (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Cell Biology 2020Quote: ... bound to a cation-exchange column (Mono S 10/100 GL, GE Healthcare) with 50mM MES ...
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bioRxiv - Immunology 2020Quote: ... with either a research grade Series S Sensor Chip Protein G (GE healthcare) or Series S Sensor Chip CM5 (GE healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... Eluted COPs were loaded onto a 1 mL Resource S column (GE Healthcare) equilibrated with COP Buffer A (50 mM sodium acetate pH 5.0 ...
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bioRxiv - Biophysics 2021Quote: ... All experiments were performed using an SA Series S Sensor Chip (GE Healthcare), which has four flow cells each containing streptavidin pre-immobilised to a carboxymethylated dextran matrix ...
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bioRxiv - Biochemistry 2020Quote: ... The Fab fragment was purified using ion exchange chromatography (Mono S, GE Healthcare; using a gradient of 10-500 mM NaCl in 20 mM sodium acetate ...
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bioRxiv - Biophysics 2020Quote: ... this dialysate was loaded onto a Resource-S cation exchange column (GE Healthcare) equilibrated in 50 mM NaCH3CO2 pH 5 and eluted with a 0-50% gradient of elution buffer (50 mM NaCH3CO2 pH 5 ...
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bioRxiv - Microbiology 2020Quote: ... the S glycoprotein was captured by StrepTactin Sepharose High Performance resin (GE Healthcare) and eluted with 10 mM D-desthiobiotin in the binding buffer (20) ...
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bioRxiv - Biochemistry 2021Quote: ... After Ub2 was purified by cation-exchange on a Mono S (GE Healthcare) column ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was further purified on a Mono S cation exchange column (GE Healthcare) and stored in CHIP TPR storage buffer (10 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... The flow-through fraction was applied to a Mono S column (GE Healthcare). Proteins were fractionated with a 0 to 500 mM linear gradient of NaCl in buffer D (6 M urea ...
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bioRxiv - Neuroscience 2022Quote: ... and 1% Penicillin/Streptomycin (P/S) (GE Healthcare Life Sciences; Logan, UT, USA). Cells were incubated at 37°C/5% CO2 and passaged using TrypLE Express (GIBCO ...
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bioRxiv - Plant Biology 2024Quote: ... The eluate was applied to a Sephacryl S-100 HR column (GE Healthcare), equilibrated and eluted with the HEPES buffer ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2,000 response units (RU) ...
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bioRxiv - Plant Biology 2022Quote: ... the culture filtrate was loaded onto HiTrap SP FF column(s) (GE Healthcare) equilibrated in buffer A (50 mM MES ...
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bioRxiv - Neuroscience 2023Quote: ... the lysate was purified using glutathione S-transferase beads (Ge healthcare, #17-5132). The proteins were eluted and concentrated with Amicon Centrifugal Filter Units 10K (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... Secreted protein was captured by two round of batch absorption (BA) with 4 mL/L of pre-equilibrated Ni Sepharose beads (GE Healthcare, Cat #17-5318-01). The bound beads were transferred to gravity flow column and sequentially washed with 30 CVs Wash Buffer (50 mM HEPES 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... pombe lysate were mixed and incubated at 4 °C for 30 min before the addition of glutathione-sepharose beads (GE Healthcare, Cat#17-0756-01). After 2-hour incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Eluate was then cleaved with TEV protease overnight at 4 °C and subsequently purified further by size exclusion chromatography (Superdex 200 26/600 from GE Healthcare; cat # GE28-9893-36) in TBS ...
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bioRxiv - Pathology 2023Quote: ... packed with Ni2+ nitrilotriacetic acid (NTA)-sepharose (GE Healthcare) were used ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) and peptides focused for 20 kVh ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) diluted 1:50 in 5% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... backside blotted for 5-10 s with Whatman filter paper grade 1 (GE Healthcare) and vitrified in liquid ethane using a home-made manual plunger.
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bioRxiv - Molecular Biology 2021Quote: ... The Flow-Through was loaded in a 1 ml RESOURCE S column (GE Healthcare), and eluted through a 25 ml gradient (100 mM-1 M NaCl) ...
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bioRxiv - Bioengineering 2021Quote: ... Further purification is performed through cation exchange (Mono S, GE Healthcare Life Sciences, USA), and the molecular weight of LwaCas13a protein (Supplementary Figure 6 ...
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bioRxiv - Bioengineering 2022Quote: ... and then by size exclusion chromatography (SEC) (Superdex S-200preparatory-grade column (GE Healthcare)) ...