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Citations for GE Life Sciences :
651 - 700 of 1323 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Sample was washed twice with HBSS and then centrifuged for 15 minutes at 1800 RPM at 4° C through 25% Percoll (GE HealthCare, Pittsburg, PA). The resulting fatty upper and fluid middle layers were removed from the pellet ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentrated supernatant was loaded on a Tricorn 10/300 Sepharose 4 Fast Flow (S4FF) size exclusion chromatography column (GE Healthcare, Buckinghamshire, UK) connected to the ÄKTA prime system (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Bacterial lysates were cleared by centrifugation at 10,000 g for 20 min at 4°C and applied to a HisTrap HP column using an AKTA Prime protein purification system (GE Healthcare, Life Sciences). Bound protein was eluted with a linear imidazole gradient ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Microbiology 2022Quote: 5 μg of input and 10 μl of output was resolved by SDS-PAGE by 4-20% acrylamide gels and analyzed by in-gel fluorescence using a Typhoon FLA 7000 (GE Healthcare Life Science) using the rhodamine channel to detect TAMRA-labeled proteins.
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were centrifuged for 20 min at 23000 × g at 4°C and filtered using Whatmann Mini-Uniprep filtering vials (GE Healthcare, Buckinghamshire, UK) with a regenerated cellulose membrane (0.2-μm pore size).
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bioRxiv - Biophysics 2020Quote: ... DCS experiments with working buffer solutions containing 4 μM of an SBD-mutant and 5 mM substrate were conducted on a VP-DSC Calorimeter (Micro Cal™, GE Healthcare, Buckinghamshire). The melting temperature Tm was determined by ORIGIN 8 (Origin Lab Corp ...
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bioRxiv - Immunology 2022Quote: ... and the resultant lysate was incubated overnight at 4 °C with 1.5 ml of glutathione-Sepharose 4B bead slurry (GE Healthcare, 17-0756-01). Beads were washed three times in wash buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... bacterial extracts were centrifugated for 20 min at 18,000 x g at 4°C and the supernatant was incubated with 1.5 ml of a GSH-sepharose 4B resin (GE Healthcare 17-0756-01) for 1.5 h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated at 4°C for at least 1 hr with ∼40 µL of appropriate bead slurry (nProtein A Sepharose, GE Healthcare, 5280-01). Following incubation ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was separated by ultracentrifugation at 100 000 x g for 30 min at 4 °C and supernatant was loaded onto a HisTrap HP column (GE Healthcare, Freiburg, Germany) preequilibrated with 50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies (anti-UBAP2L or anti-PLK1) as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Biochemistry 2023Quote: ... The antibodies raised against each AtCYT6 domain were purified from the sera using CNBr-activated Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) with immobilized recombinant AtCYT6-NTD and AtCYT6-CTD according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... on an Akta Pure chromatography system using a Tricorn 10/300 column with Sepharose 4 Fast Flow resin (all GE Healthcare Life Sciences). EVs were then sterilized by 0.45 μm syringe-filtration ...
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bioRxiv - Biochemistry 2024Quote: ... CezanneCAT was eluted by cleavage of the GST tag with 15 units/ml TEV protease (SigmaT4455-1KU) in PBS buffer overnight at 4°C and desalted using a PD-10 column (GE Healthcare, cat. # 17085101) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Synthetic Biology 2024Quote: The OVA protein was first attached to an oligonucleotide using a succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) linker and purified using a NAP column (GE Healthcare Life Sciences). Unconjugated oligonucleotides were removed by 30K Amicon filtration (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... each studied sample—the entire EC and entire MB prepared from each hemi-forebrain—was incubated with 40 µL of Protein G Sepharose 4 Fast Flow resin (GE healthcare, Piscataway, NJ) slurry (volume of settled beads:volume of storage buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was gel filtrated on Sephacryl S-200 HR (GE Healthcare) equilibrated with NH100 buffer ...
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bioRxiv - Molecular Biology 2020Quote: Ni-NTA purified S proteins were further loaded into Superdex200 (GE Healthcare), the fractions were harvested and analyzed by SDS-PAGE ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM DTT) and loaded onto a Mono S column (GE Healthcare). Bound proteins were eluted by linear NaCl gradient with buffer CL and buffer CH (20 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... at 25 °C using a Series S Sensor Chip NTA (GE Healthcare). CSP27 and CSP9 were immobilized on separate channels on the sensor chip surface as per the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... for one hour or Glutathione S-sepharose 4B (GE Healthcare Life Sciences) for 3 hours and the resin washed extensively with buffer A (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... loaded onto a mono-Q or a mono-S column (GE Healthcare), equilibrated in buffer D (buffer C containing 100 mM NaCl) ...
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bioRxiv - Biochemistry 2020Quote: ... and by gel filtration on a Superdex S-20 column (GE Healthcare). Proteins were flash-frozen in 50mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... HCoV-OC43 S was purified using a StrepTrap HP column (GE healthcare). Purified proteins were concentrated ...
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bioRxiv - Systems Biology 2021Quote: ... footprint-containing monosomes were purified via MicroSpin S-400 columns (GE Healthcare) and footprints were purified with miRNeasy Mini kit (217004 Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... were captured on a Streptavidin (SA) Series S Sensor Chip (GE Healthcare). Chip capture was performed in HBS-P+ buffer (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein L sensor chip (GE Healthcare) at a density of 400 RU and experiments were performed in HBS-EP+ buffer at pH 6.0 following the method described above ...
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bioRxiv - Biochemistry 2020Quote: ... reactions were put over a MicroSpin S-400 HR column (GE Healthcare), which was equilibrated three times with 250 ml buffer containing 25 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was gel filtrated on Sephacryl S-300 HR (GE Healthcare) equilibrated with NT350 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 150 s intervals using a MicroCal PEAQ-ITC instrument (GE Healthcare). Buffer was exchanged into 25 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2000 RU ...
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bioRxiv - Immunology 2021Quote: ... The protein was further purified with Sephacryl S-300 HR (GE Healthcare) with PBS ...
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bioRxiv - Genomics 2022Quote: ... ribosome complexes were enriched using MicroSpin S-400 HR Columns (GE Healthcare) and RNA extracted using the Direct-zol RNA Miniprep Kit (Zymo Research) ...
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bioRxiv - Biophysics 2022Quote: ... samples were loaded onto a 1 ml Resource S column (GE Healthcare) equilibrated with 20 mM MES-NaOH pH 6.0 and bound FimCA ...
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bioRxiv - Bioengineering 2022Quote: ... were captured on a Streptavidin (SA) Series S Sensor Chip (GE Healthcare). Chip capture was performed in HBS-P+ buffer (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded onto a size exclusion chromatography column (S-100, GE Healthcare). Protein purity was checked by SDS-PAGE and the identity of the purified proteins was confirmed by MALDI-TOF mass spectrometry.
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bioRxiv - Biochemistry 2022Quote: ... a cation exchange purification step (1 ml Resource S column, GE Healthcare) was introduced before size exclusion chromatography ...
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bioRxiv - Biophysics 2023Quote: ... Anion-exchange chromatography with a HiTrap S column (GE Healthcare Life Sciences) was further used to remove potent DNA/RNA contamination ...
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bioRxiv - Genetics 2024Quote: ... and was injected on a Series S Sensor Chip SA (GE Healthcare) installed in Biacore T200 ...
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bioRxiv - Immunology 2022Quote: ... Streptavidin was coupled to CM5 sensor chips using an amino coupling kit (GE Healthcare Life Sciences) to near saturation ...