Labshake search
Citations for GE Life Sciences :
951 - 1000 of 1323 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... Samples of TRIM21 CC 235 (100uL) were resolved on a Superdex S-200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min in 20 mM Tris pH 8 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Then the mixture was centrifuged for 2 mins and the supernatant was desalted by filtration with a Sephacryl S-400 HR column (GE Healthcare), centrifuged at 16 krpm ...
-
bioRxiv - Genomics 2020Quote: ... Ribosomes were treated with 60 U TruSeq Ribo Profile Nuclease to generate ribosome-protected fragments and isolated via size-exclusion with an Illustra MicroSpin S-400 HR column (GE healthcare). Ribosomal RNA was depleted from both ribosome-protected fragments and RNA-seq libraries using the Illumina Ribo-Zero Gold Kit (Human/Mouse/Rat ...
-
bioRxiv - Cell Biology 2020Quote: ... before heating at 95°C for 5 min and samples were separated by SDS-PAGE using a 4-16% acrylamide gradient gel and blotted onto nitrocellulose membranes (Hybond-C Extra, Amersham Bioscience or Optitran BA-S 85, GE Healthcare). The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako ...
-
bioRxiv - Molecular Biology 2020Quote: ... for 45 min on a nutator (25oC) and monosomes were isolated from the void volume of Illustra MicroSpin S-400 columns (GE Healthcare). Small RNAs were extracted using the miRNeasy kit (Qiagen ...
-
bioRxiv - Bioengineering 2020Quote: ... The ligase was inactivated with 2 M NaCl and the sample was injected into a Sephacryl S-1000 size exclusion column (GE Healthcare) to remove excess oligos and DNA ligase.
-
bioRxiv - Bioengineering 2020Quote: ... Fusion proteins were first immobilized at a coupling density of ∽1000 response units (RU) on a series S sensor chip protein A (GE Healthcare) in PBS and 0.02% (w/v ...
-
bioRxiv - Microbiology 2021Quote: ... The liposomes were then purified by gel filtration using a Hi Prep 16/60 Sephacryl S-300 HR column (GE Healthcare) in 150 mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... iMAC samples were thawed on ice and loaded into a purification column packed with 10 mL of Sephacryl S-100 (GE Healthcare) equilibrated with 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2020Quote: ... the concentrated fractions containing ɸEL (eluting at 8–15 mS cm-1) were subjected to size exclusion chromatography (SEC) on Sephacryl S-400 (GE Healthcare) in buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Lysates were treated with RNase I (5U/OD260) and ribosome-protected fragments were isolated via centrifugation through Microspin S-400 HR columns (GE Healthcare) and purified using the RNA Clean and Concentrator kit (Zymo Research) ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare). Approximately 1000 response units of Pf34 bait protein was captured onto the active flow cell ...
-
bioRxiv - Biophysics 2021Quote: ... The three encapsulins used in this study were purified via size exclusion chromatography (SEC) using a HiPrep 26/60 Sephacryl S-500 HR column (GE Healthcare) equilibrated with 50 mM HEPES pH 7.4 ...
-
bioRxiv - Biophysics 2021Quote: ... Fractions containing the protein of interest were pooled together and loaded onto a pre-equilibrated size exclusion column (HiPrep 16/60 Sephacryl S-300, GE Healthcare). The protein was then eluted using HSU buffer and the protein fractions were used immediately or frozen in liquid nitrogen and stored at −80°C.
-
bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
-
bioRxiv - Biochemistry 2022Quote: ... Further polishing of the complex prior SEC and after tag cleavage with PreScission protease was carried out by loading onto a cation-exchange (CE) Resource-S column (GE Healthcare) equilibrated in 10mM HEPES (pH 7.5) ...
-
bioRxiv - Immunology 2022Quote: ... and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P and ferritin) column (GE Healthcare) in 10 mM Tris pH=8 ...
-
bioRxiv - Plant Biology 2022Quote: ... The PCR product was cloned downstream and in frame with the glutathione S-transferase (GST) ORF in pGEX-3X (GE Healthcare). The expression construct was transfected into E ...
-
bioRxiv - Biochemistry 2022Quote: ... The former vector encodes a glutathione S-transferase (GST) fusion protein that can be removed proteolytically with Prescission Protease (GE Healthcare) after affinity purification ...
-
bioRxiv - Bioengineering 2022Quote: ... were immobilized on individual flow cells at 5 μl/min using a streptavidin-coated sensor chip (Series S Sensor chip SA, GE Healthcare). Three-fold serial dilutions of recombinant DLL4 proteins (N-EGF5 ...
-
bioRxiv - Immunology 2020Quote: ... Conditioned medium was clarified by centrifugation and protein was sequentially purified by a HisTrap HP column and a HiPrep 16/60 Sephacryl S-300 HR size exclusion chromatography (SEC) column (both from GE Healthcare). Purified protein was first analyzed by Native-PAGE and Western blot ...
-
bioRxiv - Immunology 2021Quote: The purification of S protein from cell broth was performed using on AKTA Pilot 600R system (GE Healthcare, Little Chalfont, UK). After harvesting ...
-
bioRxiv - Biophysics 2020Quote: ... Cleaved protein was eluted from the beads and further purified on a cation exchange column (Mono S 10/100, GE Healthcare) in MES pH 6.5 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The gel filtration was performed with a HiPrep 16/60 Sephacryl S-100 column on an AKTA chromatography system (GE Healthcare), and the size of the proteins in each eluted fraction were verified by SDS-PAGE.
-
bioRxiv - Synthetic Biology 2021Quote: ... Samples were topped up to 1 ml with Buffer A and loaded onto a HiPrep 16/60 Sephacryl S-500 HR column (GE Healthcare). Buffer A was run at 1 ml/min and the flow-through was collected in 5 ml fractions ...
-
bioRxiv - Cell Biology 2020Quote: Frozen aliquots of SARS-CoV-2 Spike trimer (Wuhan) and D614G mutant S trimer were thawed and purified by size exclusion chromatography (Superose 6 Increase 10/300, GE Healthcare) using 2 mM Tris pH 8.0 ...
-
bioRxiv - Immunology 2020Quote: ... FP3 or FP4 from the Mono S column were pooled and further purified by SEC performed on a 24 mL Superdex 200 increase column (GE Healthcare) at 4 °C with a flow rate of 0.25 mL/min in a buffer containing 20 mM HEPES ...
-
bioRxiv - Cell Biology 2020Quote: ... All capture surfaces were regenerated with one 30-s pulse of 10 mM glycine–HCl (pH 1.5, (GE Healthcare, Marlborough, MA). Kinetic parameters were obtained by globally fitting the data to a 1:1 binding model with mass transport limitation using Scrubber 2.0c Evaluation Software ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with 240 mM imidazole and further purified over a Superdex S-75 gel filtration column (GE Healthcare) equilibrated in 150 mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: For crystallization trials the ATAD2 BRD was further purified by gel-filtration using a HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare). The purified ATAD2 BRD 966-1112 ...
-
bioRxiv - Pathology 2021Quote: ... and 3500 V for 28 kVh (linear) with a Hoefer™ IEF 100 (M/s. GE Healthcare Life Sciences, Amersham, UK) at 20 °C ...
-
bioRxiv - Immunology 2020Quote: ... Purified mAbs were first immobilized at coupling densities of 800-1200 response units (RU) on a series S sensor chip protein A (GE Healthcare) in PBS and 0.02% sodium azide buffer ...
-
bioRxiv - Microbiology 2020Quote: ... the recovered recombinant proteins were further purified by size exclusion chromatography using a Sephacryl S-400 16/60 column (GE Healthcare). Fractions containing the protein were pulled ...
-
bioRxiv - Microbiology 2021Quote: Purified TlpALBD was immobilized on a CM5 series S sensor chip and binding affinities tested using a BIAcoreTM S200 instrument (GE Healthcare) as described (Day et al. ...
-
bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
-
bioRxiv - Bioengineering 2021Quote: ... Sonications (10-ms bursts applied at 2 Hz for 100 s) were applied coincident with an injection of the MB ultrasound contrast agent Optison (GE Healthcare, Little Chalfont ...
-
bioRxiv - Immunology 2023Quote: SPR measurements were performed using a Biacore T200 (GE Health-care) instrument docked with an S CM5 series sensor chip (GE Healthcare), as described (Murugan et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cat#47410-26615 or Cat#47410-07595) or with a FUSION Solo S imaging system (VILBER) using ECL reagents (GE Healthcare). Quantification was performed via densitometry using ImageJ40 ...
-
bioRxiv - Biophysics 2023Quote: VD1 hydrodynamic properties were approximated by SEC using a HiLoad 16/60 Superdex 75 prep grade (S-75) column (GE Healthcare) in 20 mM Tris ...
-
bioRxiv - Molecular Biology 2023Quote: ... Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare). Fractions of interest were pooled ...
-
bioRxiv - Biophysics 2023Quote: ... the remaining sample was centrifuged at 16,000 x g for 10 min to remove any precipitate and loaded onto a HiPrep 16/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) pre-equilibrated in PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...
-
bioRxiv - Immunology 2023Quote: ... protein was purified using cell-free culture supernatant by StrepTactin resin (IBA) and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P) column (GE Healthcare) in 10 mM Tris pH8 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The purified MUS81-EME1 was subjected to cation-exchange chromatography (CEC) with a Mono S 4.6/100 PE column (GE Life Sciences) on ÄKTA explorer FPLC system ...
-
bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... The final solution was then purified to greater than 95 % purity over a Sephadex S-300HR gel filtration column (GE Healthcare) and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... of SOD1 is further purified by size-exclusion chromatography (SEC) using a custom made gel filtration column (dimensions: id 1.2×100cm) packed with SephacrylTM S-200 (GE Healthcare, USA) in order to to isolate the dimeric protein fraction in TBS buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... monomeric actin was separated from oligomeric species by passing it through a HiPrep 26/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) equilibrated in G-buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare). The purified CapZ was flash-frozen in liquid N2 and stored at −80 °C in a buffer containing 20 mM Tris-HCl ...