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Citations for GE Life Sciences :
901 - 950 of 1323 citations for S 3 Amino 4 4 cyanophenyl butanoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The protein was then run over a size exclusion column (GE Healthcare HiPrep™ 26/60 Sephacryl S-300 HR) in sizing buffer (50mM Tris-HCl pH=8.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the pH adjusted to 5.0 with 1 M HCl and loaded on a Mono S column HR10/30 (GE Healthcare), equilibrated with 25 mM acetate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Fp231 was further purified by gel filtration (HiPrep™ 16/60 Sephacryl™ S-200 HR; GE Healthcare, Uppsala, Sweden) in 50 mM sodium citrate-phosphate pH 6.0 ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was further purified over a gel filtration column (HiPrep 16/60 Sephacryl S-100 High Resolution, GE Healthcare) equilibrated with AUC buffer (50 mM NaH2PO4 pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The flow-through from this step was concentrated in an Amicon Ultra Centrifugal Filters (10,000 MWCO, Milipore) and applied to a HiPrep 16/60 Sephacryl S-200 gel filtration column (GE Healthcare) in buffer containing 50 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2021Quote: ... The grids were blotted for 7 s at force 10 using single-layer Whatman filter papers (1442-005, GE Healthcare) before plunging into liquid ethane using an FEI MarkIV Vitrobot at 8 °C and 100% humidity ...
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bioRxiv - Microbiology 2020Quote: ... Digestion was stopped with SuperaseIN and monosomes purified by size exclusion chromatography on MicroSpin S-400 HR columns (GE Healthcare) as described (Shamimuzzaman ...
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bioRxiv - Immunology 2022Quote: ... The sensorgrams of the three rounds were aligned from 120 to 240 s in Biacore 8K Evaluation software (GE Healthcare). The blocking efficacy was determined by a comparison of the response units with and without prior antibody injection.
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bioRxiv - Biochemistry 2020Quote: ... concentrated and was followed by size exclusion chromatography (SEC) with a Superdex column (S-200, 16/60, GE life sciences) pre-equilibrated with buffer C (Tris-Cl 50 mM ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was stopped by 20-fold dilution in 50 mM sodium acetate (pH 4.5) and chains of different lengths were separated by cation exchange using a Resource S column (GE Healthcare). Elution of different chain lengths was achieved with a gradient from 0 to 600 mM NaCl.
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bioRxiv - Biochemistry 2020Quote: ... The measurements were done at flowrate of 0.5 ml/min over an S-75 Superdex 10/300 column (GE Healthcare) in 20 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... the protein precipitate was resuspended in 50 mM MOPS buffer (pH 7.0) and then applied to a Sephacryl S-100 column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Free dye was removed by size exclusion chromatography using a HiPrep 16/60 Sephacryl S 100 HR column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Biophysics 2020Quote: ... S-Trimers were purified to homogeneity by consecutive chromatographic steps including Protein A affinity column using MabSelect PrismA (GE Healthcare) which was preloaded with Endo180-Fc at (3 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... All cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque Inc.) supplemented with 10% v/v fetal bovine serum (FBS; Hyclone, GE Healthcare) and penicillin/streptomycin (Nacalai Tesque Inc. ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each protein was then purified on a HiPrep™ 16/60 Sephacryl S-500 HR gel filtration column (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... The respective reactions were stopped by 20-fold dilution in 50 mM sodium acetate (pH 4.5) and chains of different lengths were separated by cation exchange using a Resource S column (GE Healthcare). Elution of different chain lengths was achieved with a gradient from 0 to 600 mM NaCl.
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bioRxiv - Biochemistry 2023Quote: ... Cleaved protein was diluted further to 0.2M NaCl concentration and applied to cation exchange 10/10 Mono S column (GE Healthcare). Small terminase was eluted in two peaks with 0.2- 1M NaCl gradient ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved SipAC was then passed through a HiPrep 26/60 Sephacryl S-200 HR size-exclusion column (GE Healthcare) using actin polymerization buffer [F-buffer ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Optitran BA-S 85, pore size 0.45 µm, GE Healthcare) using a semi-dry blotting chamber at a constant current of 140 mA/gel for 60 minutes ...
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bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
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bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
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bioRxiv - Biophysics 2022Quote: ... The refolding mixture was dialyzed at 4 °C against 20 mM MOPS-NaOH pH 6.7 to remove residual GdmCl and applied to a Resource S column (GE Healthcare) to separate FimCHisI from free FimCHis ...
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bioRxiv - Microbiology 2022Quote: ... 2006 35 was covalently immobilized on a CM7 Series S sensor chip using an amine coupling reagent kit (GE Healthcare) at the range level of 3,000 to 5,000 response units (RU) ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Molecular Biology 2024Quote: ... Aliquots of the eluate were passed through a 0.22 µm filter and injected into a HiPrep 26/60 Sephacryl S-200 column (GE Healthcare) in buffer G1 (25 mM Tris/HCl pH 8 at RT ...
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bioRxiv - Microbiology 2024Quote: 0.5 ml of 10.6 mg/ml BoGH13BSus or 8.2 mg/ml SusA was applied to a HiPrep 16/60 Sephacryl S-200 HR (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluate was passed through a 0.22 μm filter and applied to Sephacryl S-200 26/60 column (GE Healthcare) in SEC buffer (20 mM HEPES/KOH ...
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bioRxiv - Biochemistry 2024Quote: ... The respective reactions were stopped by 20-fold dilution in 50⍰mM sodium acetate (pH 4.5) and chains of different lengths were separated by cation exchange using a Resource S column (GE Healthcare). Elution of different chain lengths was achieved with a gradient from 0 to 600⍰mM NaCl.
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion protein was purified with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The EtHAS B protein ...
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bioRxiv - Biochemistry 2020Quote: ... the protein supernatant was loaded onto a nickel-nitrilotriacetic acid (Ni-NTA) column (GE Healthcare) containing 0.1 M Ni2+ and equilibrated with the same loading buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then incubated with [3H]acetic acid (1 μCi/well; Amersham, GE Healthcare) in serum free DMEM F-12 for 4 hrs ...
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bioRxiv - Immunology 2021Quote: ... The bound protein was eluted with 100 mM acetic acid on ÄKTA pure (GE healthcare). To remove IgG1 Fc ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was purified on a Ni2+-nitrilotriacetic acid (Ni-NTA) sepharose resin (GE Healthcare), eluting with a linear gradient of imidazole (100 – 400 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each protein was purified on a 5ml nickel nitrilotriacetic acid (Ni2+-NTA) resin (GE Healthcare). The column was washed stepwise with 20 ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Biophysics 2021Quote: ... coli cells were transformed with pGEX-4T-3 (GE Healthcare) containing the coding sequences of fascin ...
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bioRxiv - Cell Biology 2020Quote: ... we used a DeltaVision|OMX microscope version 3 (GE Healthcare) with an objective lens 100X UPlanSApo NA1.40 Oil (Olympus ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~7 mg/mL ...
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bioRxiv - Developmental Biology 2020Quote: GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).
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bioRxiv - Immunology 2020Quote: ... and protein A-Sepharose (PAS, 3 mg/sample, GE Healthcare), after a preclearing step on PAS (1 h ...
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bioRxiv - Immunology 2024Quote: ... + 3 ml of isotonic Percoll (ISP; 90% Percoll (GE Healthcare) + 10% 1 M HBSS (Gibco)) ...
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bioRxiv - Biochemistry 2024Quote: ... each containing 3 mL of Heparin Sepharose resin (GE Healthcare), followed by incubation on a rocking shaker for 2.5 h at 6℃ ...
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bioRxiv - Molecular Biology 2023Quote: ... twenty-one-residue-long peptides based on MITF residues 314 to 333 were synthesized on amino-functionalized cellulose membranes (Whatman Chromatography paper Grade 1CHR, GE Healthcare Life Sciences #3001-878) and the presence of SPOTed peptides was confirmed by ultraviolet light (UV ...
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bioRxiv - Biophysics 2021Quote: ... the sample was concentrated to 2 mL and loaded onto a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Plin4 4T>S and csw 12mer were then further purified in a single step by cation exchange chromatography on a 20 ml Hiprep S HP column (GE Healthcare), as described for Plin4 AHs (Copic et al. ...
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bioRxiv - Molecular Biology 2020Quote: Purified Affimer proteins were incubated with KRAS lysates overnight at 4°C and the complexes purified by Ni-NTA affinity chromatography and size exclusion chromatography using HiPrep 16/60 Sephacryl S-100 column (GE Healthcare). The complexes were concentrated to 24 mg/ml (KRAS:K3 ...